Research article

BMC Dermatology

, 10:5

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

The role of spectrophotometry in the diagnosis of melanoma

  • Paolo A AsciertoAffiliated withNational Cancer Institute Email author 
  • , Marco PallaAffiliated withNational Cancer Institute
  • , Fabrizio AyalaAffiliated withNational Cancer Institute
  • , Ileana De MicheleAffiliated withNational Cancer Institute
  • , Corrado CaracòAffiliated withNational Cancer Institute
  • , Antonio DaponteAffiliated withNational Cancer Institute
  • , Ester SimeoneAffiliated withNational Cancer Institute
  • , Stefano MoriAffiliated withNational Cancer Institute
  • , Maurizio Del GiudiceAffiliated withNational Cancer Institute
    • , Rocco A SatrianoAffiliated withUnit of Dermatology, Second University of Naples
    • , Antonio VozzaAffiliated withUnit of Dermatology, Second University of Naples
    • , Giuseppe PalmieriAffiliated withInstitute of Biomolecular Chemistry-CNR
    • , Nicola MozzilloAffiliated withNational Cancer Institute



Spectrophotometry (SPT) could represent a promising technique for the diagnosis of cutaneous melanoma (CM) at earlier stages of the disease. Starting from our experience, we further assessed the role of SPT in CM early detection.


During a health campaign for malignant melanoma at National Cancer Institute of Naples, we identified a subset of 54 lesions to be addressed to surgical excision and histological examination. Before surgery, all patients were investigated by clinical and epiluminescence microscopy (ELM) screenings; selected lesions underwent spectrophotometer analysis. For SPT, we used a video spectrophotometer imaging system (Spectroshade® MHT S.p.A., Verona, Italy).


Among the 54 patients harbouring cutaneous pigmented lesions, we performed comparison between results from the SPT screening and the histological diagnoses as well as evaluation of both sensitivity and specificity in detecting CM using either SPT or conventional approaches. For all pigmented lesions, agreement between histology and SPT classification was 57.4%. The sensitivity and specificity of SPT in detecting melanoma were 66.6% and 76.2%, respectively.


Although SPT is still considered as a valuable diagnostic tool for CM, its low accuracy, sensitivity, and specificity represent the main hamper for the introduction of such a methodology in clinical practice. Dermoscopy remains the best diagnostic tool for the preoperative diagnosis of pigmented skin lesions.