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Application of Indirect Enzymatic Method for Determinations of 2-/3-MCPD-Es and Gly-Es in Foods Containing fats and Oils

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Journal of the American Oil Chemists' Society

Abstract

Because of the potential health risks, fatty acid esters of 3-chloro-1,2-propanediol (3-MCPD-Es), 2-chloro-1,3-propanediol (2-MCPD-Es) and glycidol (Gly-Es) in foods are drawing the attention of public health authorities. To assess applicability of the rapid indirect method developed earlier by using a Candida rugosa lipase for the analyses of refined fats and oils was applied to the analyses of various foods. Mayonnaise, vegetable oil margarine and fat spread could be analyzed with the hydrolysis condition of 30 min at room temperature. Analyses of 3-MCPD-Es in margarines and fat spreads containing milk fat could be analyzed by increasing the hydrolysis temperature to 40 °C. The results in a mayonnaise, four fat spreads and five margarines analyzed by the enzymatic method were 0.10–0.98 mg/kg for 3-MCPD, 0.05–0.41 mg/kg for 2-MCPD and 0.15–0.59 mg/kg for Gly, and correlated well with the results obtained by AOCS Cd 29a with Cd 30–15 with slopes of 0.99–1.13, and R 2s of 0.87–0.99. Further, by adding a simple fat extraction step using a solvent mix at 60 °C, foods high in protein and carbohydrate, such as infant formulas, could also be successfully analyzed with >90 % recovery in 1 day. Because the enzymatic method requires only 30 min for hydrolysis, the method is considered suitable for routine analyses of 2-/3-MCPD-Es and Gly-Es in foods.

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  1. House Foods Group Inc., 1-4 Takanodai, Yotsukaido, Chiba, 284-0033, Japan

    Kinuko Miyazaki & Kazuo Koyama

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  1. Kinuko Miyazaki
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  2. Kazuo Koyama
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Correspondence to Kinuko Miyazaki.

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Miyazaki, K., Koyama, K. Application of Indirect Enzymatic Method for Determinations of 2-/3-MCPD-Es and Gly-Es in Foods Containing fats and Oils. J Am Oil Chem Soc 93, 885–893 (2016). https://doi.org/10.1007/s11746-016-2833-6

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  • DOI: https://doi.org/10.1007/s11746-016-2833-6

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