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Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.)

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Abstract

An effective pistachio (Pistacia vera L.) micropropagation system was developed involving rapid axillary bud proliferation and ex vitro rooting. The highest shoot proliferation frequency was obtained from nodal explants cultured on Murashige and Skoog (MS) basal salts containing Gamborg (B5) vitamins and supplemented with 4 mg l−1 6-benzyladenine (BA). The addition of 2 mg l−1 meta-topolin (mT) generated an optimal number of shoots with suitable morphological features, while kinetin (KIN) was found to be unsuitable for pistachio shoot proliferation. Microcuttings were rooted ex vitro after being dipped in rooting powder. The peak ex vitro rooting response was achieved after shoot explants were treated with Rhizopon® 2% indole-3-butyric acid (IBA). Rooted plantlets were transplanted in plastic pots containing a peat–perlite–vermiculite (1:1:1) mixture and then transferred to the greenhouse. After 2 months, 81.5% survival of rooted microshoots was achieved.

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Fig. 1

Abbreviations

BA:

6-Benzyladenine

B5:

Medium described by Gamborg et al. (1968)

IBA:

Indole-3-butyric acid

KIN:

Kinetin

mT:

meta-Topolin

MS:

Murashige and Skoog medium (1962)

NAA:

Naphthaleneacetic acid

PGR:

Plant growth regulator

SE:

Standard error

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Acknowledgments

This research benefitted from the support of the Ministry of Higher Education and Scientific Research (Algeria) and a grant from the Centre National des Oeuvres Universitaires et Sociales (CNOUS-France) for B. Benmahioul.

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Authors and Affiliations

  1. Département des Sciences Agronomiques et Forestières, Faculté des Sciences de la Nature et de la Vie & Sciences de la Terre et de l’Univers (SNV-STU), Université «Abou Bekr Bel KAID», BP 119, Tlemcen, 13000, Algeria

    Benamar Benmahioul

  2. Département de Biotechnologies, Faculté des Sciences, Université des Sciences et de la Technologie «Mohammed BOUDIAF», BP 1505, El Mnaouar, Oran, Algeria

    Benamar Benmahioul & Meriem Kaid-Harche

  3. AGROCAMPUS-OUEST, Centre d’Angers, Institut National d’Horticulture et de Paysage, 2 rue Le Nôtre, 49045, Angers Cedex 01, France

    Benamar Benmahioul, Noëlle Dorion & Florence Daguin

Authors
  1. Benamar Benmahioul
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  2. Noëlle Dorion
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  3. Meriem Kaid-Harche
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  4. Florence Daguin
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Corresponding authors

Correspondence to Benamar Benmahioul or Florence Daguin.

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Benmahioul, B., Dorion, N., Kaid-Harche, M. et al. Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.). Plant Cell Tiss Organ Cult 108, 353–358 (2012). https://doi.org/10.1007/s11240-011-0040-6

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  • DOI: https://doi.org/10.1007/s11240-011-0040-6

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