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Cell'Cell Interactions

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Sean P. Colgan

    1. Center for Experimental Therapeutics and Reperfusion Injury, Brigham
      Women’s Hospital, Havard Medical School, Boston, USA

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Part of the book series: Methods in Molecular Biology (MIMB, volume 341)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements

    • Michael Aschner, Vanessa A. Fitsanakis, Ana Paula Marreilha dos Santos, Luisa Olivi, Joseph P. Bressler
    Pages 1-15

  3. Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences

    • Geraldine Canny, Alexander Swidsinski, Beth A. McCormick
    Pages 17-35

  4. The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells

    • Triantafyllos Chavakis, Valeria Orlova
    Pages 37-50

  5. Cell-Cell Interactions on Solid Matrices

    • Nancy A. Louis, Dionne Daniels, Sean P. Colgan
    Pages 51-59

  6. Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein α-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function

    • Ernesto Sabath, Bradley M. Denker
    Pages 61-72

  7. Nucleotide Metabolism and Cell-Cell Interactions

    • Holger K. Eltzschig, Thomas Weissmüller, Alice Mager, Tobias Eckle
    Pages 73-87

  8. Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization

    • Genevieve B. Wortzman, Allison J. Gardner, Janice P. Evans
    Pages 89-101

  9. Collagen Gel Contraction Assay

    • Peter Ngo, Punitha Ramalingam, Jonathan A. Phillips, Glenn T. Furuta
    Pages 103-109

  10. Methods to Assess Tissue Permeability

    • Juan C. Ibla, Joseph Khoury
    Pages 111-117

  11. Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy

    • Kimberly K. Jefferson, Nuno Cerca
    Pages 119-126

  12. A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum

    • Heidi E. De Luca, Wayne I. Lencer
    Pages 127-139

  13. Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems

    • Yaw-Chyn Lim, Francis W. Luscinskas
    Pages 141-154

  14. Expression Cloning of Signaling Proteins Regulated by Cell Adhesion

    • Michelle L. Matter, Joe W. Ramos
    Pages 155-165

  15. Assays for the Calcium Sensitivity of Desmosomes

    • Anita J. Merritt, Anthea Scothern, Tanusree Bhattacharyya
    Pages 167-183

  16. Tight Junctions and Cell–Cell Interactions

    • Markus Utech, Matthias Brüwer, Asma Nusrat
    Pages 185-195

  17. Simultaneous Immunofluorescence Detection of Cadherin and β-Catenin in Mouse Tissue Sections

    • Nobuko Obara
    Pages 197-204

  18. In Vitro Neutrophil Transepithelial Migration

    • Winston Y. Lee, Alex C. Chin, Susan Voss, Charles A. Parkos
    Pages 205-215

  19. The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions

    • Robert Sackstein, Robert Fuhlbrigge
    Pages 217-226

  20. Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators

    • Nan Chiang, Charles N. Serhan
    Pages 227-250
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Keywords

About this book

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • Center for Experimental Therapeutics and Reperfusion Injury, Brigham

    Sean P. Colgan

  • Women’s Hospital, Havard Medical School, Boston, USA

    Sean P. Colgan

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