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  1. No Access


    Purification Method for Recombinant hG-CSF by Affinity Chromatography

    The human granulocytic colony-stimulating factor (hG-CSF) acts mainly by promoting the maturation of granulocytes and stimulating their phagocytic and chemotactic activity. It has been used in the treatment of...

    Bruna Samham Archangelo, Elisa Maria de Sousa Russo in Recombinant Glycoprotein Production (2018)

  2. No Access


    Modification of Selenoprotein mRNAs by Cap Tri-methylation

    Several selenoprotein mRNAs undergo 5′ cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m3 2,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunop...

    Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang in Selenoproteins (2018)

  3. No Access


    Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies

    Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formula...

    Flávia Sousa, Pedro Fonte, Andreia Cruz in Recombinant Glycoprotein Production (2018)

  4. No Access


    Computer-Aided Drug Design Approaches to Study Key Therapeutic Targets in Alzheimer’s Disease

    Alzheimer’s Disease (AD) is one of the most common and complex age-related neurodegenerative disorders in elderly people. Currently there is no cure for AD, and available therapeutic alternatives only improve ...

    Agostinho Lemos, Rita Melo, Irina S. Moreira in Computational Modeling of Drugs Against Al… (2018)

  5. No Access


    Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato Using Molecular Biological Methods

    Bacterial species identification is required in different disciplines and—depending on the purpose—levels of specificity or resolution of typing may vary. Nowadays, molecular methods are the mainstay for bacte...

    Gabriele Margos, Isabell Notter, Volker Fingerle in Borrelia burgdorferi (2018)

  6. No Access


    Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

    Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations ...

    Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad in Cancer Stem Cells (2018)

  7. No Access


    Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

    This article will outline surveillance approaches for viral hemorrhagic fevers. Specific methods for surveillance of clinical samples will be emphasized. Separate articles will describe methods for surveillanc...

    Carolina Montoya-Ruiz, Juan David Rodas Ph.D. in Hemorrhagic Fever Viruses (2018)

  8. No Access


    Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma

    Bladder cancer diagnosis and surveillance is mainly based on cystoscopy and urine cytology. However, both methods have significant limitations; urine cytology has a low sensitivity for low-grade tumors, while ...

    Tibor Szarvas Ph.D., Péter Nyirády M.D., Ph.D., D.Sc. in Urothelial Carcinoma (2018)

  9. No Access


    Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer

    The PI3K/AKT/mTOR signaling pathway shows frequent molecular and increased activity in cancer. Given its role in the regulation of cell growth, survival and metastasis, molecules within this pathway are prom...

    Anuja Sathe, Roman Nawroth in Urothelial Carcinoma (2018)

  10. No Access


    Defining the Pathways of Urogenital Schistosomiasis-Associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models

    Urogenital schistosomiasis (infection with Schistosoma haematobium) is a major cause of bladder carcinogenesis. However, the exact mechanisms of the sequelae leading up to the development of bladder cancer are po...

    Evaristus C. Mbanefo, Michael H. Hsieh M.D., Ph.D. in Urothelial Carcinoma (2018)

  11. No Access


    Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum

    Selenium is an essential element incorporated to different proteins with important biological functions in connection to antioxidant activity, cancer-protective properties, neurodegenerative pathologies, and p...

    Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera in Selenoproteins (2018)

  12. No Access


    Assays to Assess Arenaviral Glycoprotein Function

    Arenaviruses, such as Lassa virus (LASV) and Pichindé virus (PICV), are enveloped viruses with a bi-segmented ambisense RNA genome. The large (L) genomic segment encodes the Z matrix protein and the L RNA-depe...

    Junjie Shao, Xiaoying Liu, Yuying Liang, Hinh Ly in Hemorrhagic Fever Viruses (2018)

  13. No Access


    Genome-Wide Mutagenesis in Borrelia burgdorferi

    Signature-tagged mutagenesis (STM) is a functional genomics approach to identify bacterial virulence determinants and virulence factors by simultaneously screening multiple mutants in a single host animal, and...

    Tao Lin, Lihui Gao in Borrelia burgdorferi (2018)

  14. No Access


    Two Photon Intravital Microscopy of Lyme Borrelia in Mice

    Two-photon intravital microscopy is a powerful tool that allows visualization of cells in intact tissues in a live animal in real time. In recent years, this advanced technology has been applied to understand ...

    Alexia A. Belperron Ph.D., Jialing Mao, Linda K. Bockenstedt in Borrelia burgdorferi (2018)

  15. No Access


    Atomic Force Microscopy: An Introduction

    Imaging of nano-sized particles and sample features is crucial in a variety of research fields. For instance in biological sciences, where it is paramount to investigate structures at the single particle level...

    Melissa C. Piontek, Wouter H. Roos in Single Molecule Analysis (2018)

  16. No Access


    Dynamic Architecture of Eukaryotic DNA Replication Forks In Vivo, Visualized by Electron Microscopy

    The DNA replication process can be heavily perturbed by several different conditions of genotoxic stress, particularly relevant for cancer onset and therapy. The combination of psoralen crosslinking and electr...

    Ralph Zellweger, Massimo Lopes in Genome Instability (2018)

  17. No Access


    Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing

    Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequ...

    Clinton D. Orebaugh, Scott A. Lujan, Adam B. Burkholder in Genome Instability (2018)

  18. No Access


    Detection of DNA Methylation by Whole-Genome Bisulfite Sequencing

    DNA methylation plays an important role in the regulation of the expression of transposons and genes. Various methods have been developed to assay DNA methylation levels. Bisulfite sequencing is considered to ...

    Qing Li, Peter J. Hermanson, Nathan M. Springer in Maize (2018)

  19. No Access


    Purification and Autoactivation Method for Recombinant Coagulation Factor VII

    Recombinant coagulation factor VII is a very important and complex protein employed for treatment of hemophiliac patients (hemophilia A/B) who develop inhibitors antibodies to conventional treatments (FVIII an...

    Vladimir Granovski, Marcela C. C. Freitas in Recombinant Glycoprotein Production (2018)

  20. No Access


    Quantification of Coagulation Factor VIII by Selective Reaction Monitoring

    Coagulation factor VIII (FVIII) is an important glycoprotein involved in the extrinsic coagulation cascade. Mutations in FVIII gene results in hemophilia A, a recessive coagulation disorder that is clinically ...

    Edson Galdino do Nascimento Filho in Recombinant Glycoprotein Production (2018)

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