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  1. No Access

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    Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes

    BDNF gene has a complex structure and is regulated by multiple time- and stimulus-dependent promoters and distal regulatory elements. To better understand the interplay between these regulatory units, it is be...

    Kaur Jaanson, Angela Pärn, Tõnis Timmusk (2018)

  2. No Access

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    Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research

    Cancer-associated fibroblasts (CAFs) represent a crucial component of cancer microenvironment. CAFs significantly influence biological properties of various types of cancer in terms of local aggressiveness, re...

    Barbora Dvořánková, Lukáš Lacina, Karel Smetana Jr. (2018)

  3. No Access

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    Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

    Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental...

    L. M. L. Laurens, J. L. Olstad, D. W. Templeton (2018)

  4. No Access

    Protocol

    Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells

    The cancer stem cell model states that a subset of tumor cells, called “cancer stem cells,” can initiate and propagate tumor growth through self-renewal, high proliferative capacity, and their ability to recre...

    Huw Morgan, Carlotta Olivero, Girish K. Patel (2018)

  5. No Access

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    Glutaraldehyde Cross-linking Modification of Decellularized Rat Kidney Scaffolds

    The decellularized (DC) scaffolds retain three-dimensional structures for the stimulation of cell growth, with components of the extracellular matrix (ECM) relatively conserved between species. The strategy ba...

    Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, Jin Mei (2018)

  6. No Access

    Protocol

    Optimization for Peptide Sample Preparation for Urine Peptidomics

    Analysis of native or endogenous peptides in biofluids can provide valuable insight into disease mechanisms. Furthermore, the detected peptides may also have utility as potential biomarkers for noninvasive mon...

    Tara K. Sigdel, Carrie D. Nicora, Wei-Jun Qian, Minnie M. Sarwal (2018)

  7. No Access

    Protocol

    A Microfluidic Culture Platform for Neurotrophin Signaling Studies

    Brain-derived neurotrophic factor (BDNF) has a crucial role in neuronal development, maintenance, and function. Some of the effects induced by the neurotrophins occur at neuronal sub-domains, which are located...

    Rui O. Costa, Tânia Perestrelo, Diogo Tomé, Ramiro D. Almeida (2018)

  8. No Access

    Protocol

    Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice

    In this article, we provide a method to isolate primary epidermal melanocytes from reporter mice, which also allow targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26 ...

    Lina Dagnino, Melissa Crawford (2018)

  9. No Access

    Protocol

    Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, and ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia

    Immunofluorescence is an invaluable technique widely used in cell biology. This technique allows visualization of the subcellular distribution of different target proteins or organelles, by specific recognitio...

    Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin, Yongquan Hu, Tian Meng, Baojin Wu (2018)

  10. No Access

    Protocol

    Determining Inorganic and Organic Nitrogen

    Nitrogen (N) is one of the key nutrients for algal growth and is an integral part of many cellular components, for example in proteins. Being able to determine the inorganic and organic pools of N is consequen...

    Jaana Koistinen, Mervi Sjöblom, Kristian Spilling (2018)

  11. No Access

    Protocol

    Isolation and Cultivation of Epidermal (Stem) Cells

    Recent studies have shown that epidermal stem cells derived from the epidermis of are able to form hair follicles in the presence of hair follicle-inducing cells. Here we describe the method that we have used ...

    Xusheng Wang, Shiyang Dong, Yaojiong Wu (2018)

  12. No Access

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    Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells

    Epithelia are under constant threat from environmental carcinogens and none more so than squamous epithelia, which form the outermost linings of our bodies. Hence malignancies of squamous epithelia are collect...

    Carlotta Olivero, Huw Morgan, Girish K. Patel (2018)

  13. No Access

    Protocol

    Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis

    The interfollicular epidermis regenerates from a heterogeneous population of basal cells undergoing either self-renewal or terminal differentiation, thereby balancing cell loss in tissue turnover or in wound r...

    Stefano Sol, Dario Antonini, Caterina Missero (2018)

  14. No Access

    Protocol

    Determining Dissolved and Biogenic Silica

    Most algae do not use silicon in any form with one notable exception, diatoms. Silicon is a major constituent of diatoms. Diatoms are characterized by high growth rates and are often one of the key groups in f...

    Jaana Koistinen, Mervi Sjöblom, Kristian Spilling (2018)

  15. No Access

    Protocol

    Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay

    In recent years, hundreds of candidate protein biomarkers have been identified using discovery-based proteomics. Despite the large number of candidate biomarkers, few proteins advance to clinical validation. H...

    Emily M. Mackay, Oliver F. Bathe (2018)

  16. No Access

    Protocol

    Use of U-STELA for Accurate Measurement of Extremely Short Telomeres

    Telomeres are repetitive genetic materials that protect the chromosomes by capping the ends of chromosomes. Each time a cell divides, telomeres get shorter. Telomere length is mainly maintained by telomerase. ...

    Nedime Serakinci, Huseyin Cagsin, Merdiye Mavis (2018)

  17. No Access

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    Design and Analysis of Offshore Macroalgae Biorefineries

    Displacing fossil fuels and their derivatives with renewables, and increasing sustainable food production are among the major challenges facing the world in the coming decades. A possible, sustainable directio...

    Alexander Golberg, Alexander Liberzon, Edward Vitkin, Zohar Yakhini (2018)

  18. No Access

    Protocol

    Synergistic Active Targeting to B16F10 Tumors by αvβ3/CD44-Targeted Nanoparticles Loaded with Docetaxel

    Generally, many drugs including nanomedicine can permeate into a superficial layer of tumors through an enhanced permeability and retention (EPR) effect. However, blood-tumor barrier negatively impacts permeat...

    Sanjun Shi (2018)

  19. No Access

    Protocol

    NIR Imaging-Guided Photothermal Therapy by cRGD-Conjugated Solid Lipid Nanoparticles Encapsulating IR-780

    It is extremely demanded to enhance the accumulation of near-infrared theranostic agents in the tumor region, which is favorable to the effective phototherapy. Compared with indocyanine green (a clinically app...

    Ye Kuang, Kunchi Zhang, Min Liu, Renjun Pei (2018)

  20. No Access

    Protocol

    Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer

    Stem cells have the potential to differentiate into specialized cell types under specific conditions in vivo or in vitro, which are used to cure many diseases related to aging. Somatic cell nuclear transfer (S...

    Dan-Ya Wu, Xia Zhang, Yi-Liang Miao (2018)

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