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  1. No Access

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    Complimentary Methods for Assessing Hypothalamic Actions of Manganese During Pubertal Development

    Utilizing both in vitro and in vivo physiological approaches can be beneficial when trying to obtain critical insights regarding both endogenous and exogenous factors that may control or alter hypothalamic mec...

    William L. Dees, Jill K. Hiney, Vinod K. Srivastava (2018)

  2. No Access

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    Historeceptomics: Integrating a Drug’s Multiple Targets (Polypharmacology) with Their Expression Pattern in Human Tissues

    Historeceptomics is a new, integrative informatics approach to describing the mechanism of action of drugs in a holistic, in vivo context. The approach is based on leveraging emerging big data sources in genom...

    Timothy Cardozo (2018)

  3. No Access

    Protocol

    Multitarget Drug Design for Neurodegenerative Diseases

    The quest for new pharmacological treatments of neurodegenerative diseases (NDs) still remains a priority for researchers and caregivers. Being inherently multifactorial, NDs benefited of the paradigm shift fr...

    Marco Catto, Daniela Trisciuzzi, Domenico Alberga, Giuseppe Felice Mangiatordi (2018)

  4. No Access

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    Advanced Chemometric Modeling Approaches for the Design of Multitarget Drugs Against Neurodegenerative Diseases

    Neurodegenerative diseases (ND), a major worldwide health problem, present a multifactorial nature. This implies that a multitargeted therapy approach can be considered more effective in such cases when compar...

    Amit Kumar Halder, Ana S. Moura, M. Natália D. S. Cordeiro (2018)

  5. No Access

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    Design of Novel Dual-Target Hits Against Malaria and Tuberculosis Using Computational Docking

    Drugs which are purposefully designed to hit more than one target (multi-target drugs) promise a better safety profile and low resistance probability. Multi-target therapy also offers a cost-effective model fo...

    Manoj Kumar, Anuj Sharma (2018)

  6. No Access

    Protocol

    Interactions Between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells

    The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk c...

    Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, Colin Jamora (2018)

  7. No Access

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    Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells

    An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent sta...

    Edwige Roy, Kiarash Khosrotehrani (2018)

  8. No Access

    Protocol

    Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)

    Age-related macular degeneration (AMD) is the foremost cause of blindness in people over the age of 60 worldwide. Clinically, this disease starts with distortion in central vision eventually leading to legal b...

    Harshini Surendran, Reena J. Rathod, Rajarshi Pal (2018)

  9. No Access

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    Cheminformatics Approaches to Study Drug Polypharmacology

    Herein is presented a tutorial overview on selected chemoinformatics methods useful for assembling, curating/preparing a chemical database, and assessing its diversity and chemical space. Methods for evaluatin...

    J. Jesús Naveja, Fernanda I. Saldívar-González, Norberto Sánchez-Cruz (2018)

  10. No Access

    Protocol

    Development of a Web-Server for Identification of Common Lead Molecules for Multiple Protein Targets

    Due to increasing unresponsiveness of drugs to single targets in the form of resistance or presence of alternate mechanisms in case of complex diseases and disorders, etc., the focus is shifting towards polyph...

    Abhilash Jayaraj, Ruchika Bhat, Amita Pathak, Manpreet Singh, B. Jayaram (2018)

  11. No Access

    Protocol

    Strategies for Multi-Target Directed Ligands: Application in Alzheimer’s Disease (AD) Therapeutics

    Design of multi-target directed ligand (MTDL) is believed to be a novel and improved approach for diseases that elucidate a multifactorial nature. Alzheimer’s disease (AD) is related to increased levels of the...

    Sucharita Das, Soumalee Basu (2018)

  12. No Access

    Protocol

    Assays to Monitor Aggrephagy in Drosophila Brain

    Accumulation of ubiquitinated protein aggregates is a hallmark of most aging-related neurodegenerative disorders. Autophagy has been found to be involved in the selective clearance of these protein aggregates,...

    Anne-Claire Jacomin, Ioannis P. Nezis (2018)

  13. No Access

    Protocol

    Monitoring and Measuring Mammalian Autophagy

    Macroautophagy (autophagy) is a conserved lysosomal-based intracellular degradation pathway. Here, we present different methods used for monitoring autophagy at cellular level. The methods involve Atg8/LC3 det...

    Sujit K. Bhutia, Prakash P. Praharaj, Chandra S. Bhol, Debasna P. Panigrahi (2018)

  14. No Access

    Protocol

    Assessing Muscle Stem Cell Clonal Complexity During Aging

    Changes in muscle stem cell (MuSC) function during aging have been assessed using various in vivo and ex vivo systems. However, changes in clonal complexity within the aged MuSC pool are relatively understudie...

    Matthew T. Tierney, Michael J. Stec, Alessandra Sacco (2018)

  15. No Access

    Protocol

    Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

    Electrophoretic mobility shift assays and Western blots are simple, efficient, and rapid methods to study DNA–protein interactions and protein expression, respectively. Primary cultures and subcultures of epit...

    Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, Sylvain L. Guérin (2018)

  16. No Access

    Protocol

    Dopaminergic Neuron-Specific Autophagy-Deficient Mice

    None of the current genetic Parkinson’s disease (PD) models in mouse recapitulates all features of PD. Additionally, only a few of these models develop mild dopamine (DA) neurodegeneration. And the most parsim...

    Shigeto Sato, Nobutaka Hattori (2018)

  17. No Access

    Protocol

    Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells

    Neural crest cells (NCCs) are multipotent cells that emerge from the edges of the neural folds and extensively migrate throughout developing embryos. Dorsolaterally migrating NCCs colonize skin, differentiate ...

    Tsutomu Motohashi, Takahiro Kunisada (2018)

  18. No Access

    Protocol

    Skin Wound Healing: Refractory Wounds and Novel Solutions

    This overview of the current state of skin wound healing includes in vitro and in vivo approaches along with some recent clinical trials. From an introduction to wound healing, to tissue engineering as applied...

    Gabriel M. Virador, Lola de Marcos, Victoria M. Virador (2018)

  19. No Access

    Protocol

    High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy

    Lentiviral vectors are becoming preferred vectors of choice for clinical gene therapy trials due to their safety, efficacy, and the long-term gene expression they provide. Although the efficacy of lentiviral v...

    Hazal Banu Olgun, Hale M. Tasyurek, Ahter Dilsad Sanlioglu, Salih Sanlioglu (2018)

  20. No Access

    Protocol

    Isolating Immune Cells from Mouse Embryonic Skin

    Skin is the primary barrier against the external environment and develops a robust immune network for its surveillance. The origin of the resident immune cells of the skin has become a focus of interest over p...

    Ambika S. Kurbet, Srikala Raghavan (2018)

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