46,553 Result(s)

within Protocol

Relevance Newest First Oldest First
previous Page of 2,328
and
  1. No Access

    Protocol

    Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines

    Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), dia...

    Gary M. Reynolds, Barbara Visentin, Roger Sabbadini (2017)

  2. No Access

    Protocol

    Decellularization Methods for Scaffold Fabrication

    Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mecha...

    Sweta K. Gupta, Narayan C. Mishra (2017)

  3. No Access

    Protocol

    An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity

    Sphingosine kinases (SK) are the sole enzymes responsible for the production of sphingosine 1-phosphate (S1P). S1P is a signaling molecule with a plethora of targets, acting as both a second messenger intracel...

    Melissa R. Pitman, Lorena T. Davies, Stuart M. Pitson (2017)

  4. No Access

    Protocol

    Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation

    Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embr...

    Guanghu Wang, Stefka D. Spassieva, Erhard Bieberich (2017)

  5. No Access

    Protocol

    Alveolar Rhabdomyosarcoma Decellularization

    In cancer research, it is an urgent need in the obtainment of a simple and reproducible model that mimics in all the complexity the pathological microenvironment. Specifically, the will to improve the overall ...

    Michela Pozzobon, Mattia Saggioro, Stefania D’Agostino, Gianni Bisogno, Maurizio Muraca (2017)

  6. No Access

    Protocol

    Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation

    In tissue engineering, decellularized scaffolds have been proved to have remarkable capacity to promote regeneration in various organs such as kidney, heart, lung, and liver. Marrying the field of cryobiology ...

    Enes Taylan, Kutluk Oktay (2017)

  7. No Access

    Protocol

    Effects of Chemicals on Mammary Gland Development

    The mammary gland is exceptionally a complex tissue. It is a sexually dimorphic organ in function, size, response to hormone signaling, and cellular structure. Unlike most organs, the mammary gland has several...

    Adam J. Filgo, Ali S. Faqi (2017)

  8. No Access

    Protocol

    Short Overview

    Mitochondrial autophagy (mitophagy) is a mitochondrial quality control mechanism that selectively removes damaged mitochondria via autophagic degradation. Autophagic adaptor/receptor proteins contribute to the...

    Norihiko Furuya (2017)

  9. No Access

    Protocol

    Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells

    Mast cells produce a potently bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) constitutively and upon activation. The ligation of S1P to its type 2 receptor on mast cells triggers a novel downs...

    Alena P. Chumanevich, Piper A. Wedman, Carole A. Oskeritzian (2017)

  10. No Access

    Protocol

    Targeting Glioma Cancer Cells with Fluorescent Nanodiamonds via Integrin Receptors

    Glioblastomas, aggressive and highly vascularized brain tumors, overexpress αvβ3 integrins, which are widely exploited for cancer diagnostics and therapy. Proteins and peptides containing the RGD sequence bind αv

    Jitka Neburkova, Miroslav Hajek, Ivan Rehor, Jiri Schimer, Frantisek Sedlak, Jan Stursa (2017)

  11. No Access

    Protocol

    Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component Within Hydrogels as In Vitro Models and Cell Delivery Systems

    Decellularized tissues represent promising biomaterials, which harness the innate capacity of the tissue-specific extracellular matrix (ECM) to direct cell functions including stem cell proliferation and linea...

    Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, Lauren E. Flynn (2017)

  12. No Access

    Protocol

    3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

    Millions of patients worldwide require bone grafts for treatment of large, critically sized bone defects from conditions such as trauma, cancer, and congenital defects. Tissue engineered (TE) bone grafts have ...

    Alexandra N. Rindone, Ethan Nyberg, Warren L. Grayson (2017)

  13. No Access

    Protocol

    Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

    Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich ...

    Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer (2017)

  14. No Access

    Protocol

    Monitoring of Iron Depletion-Induced Mitophagy in Pathogenic Yeast

    Mitophagy, which is the degradation of mitochondria via selective autophagic machinery, is thought to be involved in regulating the mass and function of mitochondria. Methods for detection of mitophagy have be...

    Koichi Tanabe, Minoru Nagi (2017)

  15. No Access

    Protocol

    Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells

    Mitophagy is a cellular process of autophagy-based mitochondrial degradation that eliminates dysfunctional mitochondria and ensures cellular homeostasis. In pancreatic islet β-cells, mitochondria play a pivota...

    Atsushi Hoshino, Satoaki Matoba (2017)

  16. No Access

    Protocol

    S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses

    Angiogenesis is the process of new blood vessel growth from pre-existing structures. During sprout initiation, endothelial cells (ECs) are activated by pro-angiogenic factors to degrade the basement membrane, ...

    Camille L. Duran, Roland Kaunas, Kayla J. Bayless (2017)

  17. No Access

    Protocol

    Measuring miRNA Mediated Translational Regulation with Live Cell Imaging

    Numerous microRNAs are detected in synaptic areas such as axons and dendrites. As accurate regulation of local protein synthesis can be crucial for the function of neurons, miRNAs can play very important roles...

    Min Jeong Kye (2017)

  18. No Access

    Protocol

    A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes

    Sphingosine-1-phosphate (S1P) is an intercellular signaling molecule that is present in the plasma and plays an important role in recruiting lymphocytes from the thymus and secondary lymphoid organs. Erythrocy...

    Naoki Kobayashi, Tsuyoshi Nishi (2017)

  19. No Access

    Protocol

    Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

    Decellularization of whole porcine hearts followed by recellularization with fully differentiated cells made from patient-specific human induced pluripotent stem cells (iPSCs) may provide the ultimate solution...

    Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, Alonzo D. Cook (2017)

  20. No Access

    Protocol

    Preparation of Scaffolds from Decellularized Testicular Matrix

    Biological scaffolds composed of extracellular matrix (ECM) are typically derived by processes that involve decellularization of tissues or organs. Here we describe a simple and robust methodology for the prep...

    Yoni Baert, Ellen Goossens (2017)

previous Page of 2,328