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  1. No Access

    Protocol

    Methods for Monitoring Autophagy in Silkworm Organs

    In holometabolous insects, various larval organs are remodeled by autophagy during metamorphosis. Although moths and butterflies are among the first animal models in which this self-eating process was describe...

    Aurora Montali, Morena Casartelli, Daniele Bruno, Annalisa Grimaldi (2018)

  2. No Access

    Protocol

    Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury

    Traumatic brain injury (TBI) displays cognitive and motor symptoms following the initial injury which can be exacerbated by secondary cell death. Aging contributes significantly to the morbidity of TBI, with h...

    Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko (2018)

  3. No Access

    Protocol

    Monitoring Microalgal Neutral Lipid Accumulation with Nile Red

    The widely applied Nile red (NR) method allows near real-time monitoring of microalgal neutral lipid accumulation. When added to a culture sample, optimally, the fluorescent dye NR penetrates the microalgal ce...

    Katariina Natunen (2018)

  4. No Access

    Protocol

    Immunoprecipitation of RNA:DNA Hybrids from Budding Yeast

    During transcription, the nascent transcript behind an elongating RNA polymerase (RNAP) can invade the DNA duplex and hybridize with the complementary DNA template strand, generating a three-stranded “R-loop” str...

    Aziz El Hage, David Tollervey in DNA Topoisomerases (2018)

  5. No Access

    Protocol

    Site-Specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction

    Here, we describe a two-step protocol for selective protein labeling based on enzyme-mediated peptide labeling utilizing lipoic acid ligase (LplA) and bioorthogonal chemistry. The method can be applied to puri...

    Mathis Baalmann, Marcel Best, Richard Wombacher in Noncanonical Amino Acids (2018)

  6. Protocol

    Erratum to: Functional Analysis of Membrane Proteins Produced by Cell-Free Translation

    Srujan Kumar Dondapati, Doreen A. Wüstenhagen, Stefan Kubick in Protein Engineering (2018)

  7. No Access

    Protocol

    Purification Method for Recombinant hG-CSF by Affinity Chromatography

    The human granulocytic colony-stimulating factor (hG-CSF) acts mainly by promoting the maturation of granulocytes and stimulating their phagocytic and chemotactic activity. It has been used in the treatment of...

    Bruna Samham Archangelo, Elisa Maria de Sousa Russo in Recombinant Glycoprotein Production (2018)

  8. No Access

    Protocol

    Transmembrane Signal Transduction in Bacterial Chemosensing

    Like all living organisms, bacteria must communicate with the world around them. As they typically live as single cells, the communication with their environment must occur at the cell membrane, both in moving...

    Michael D. Manson in Bacterial Chemosensing (2018)

  9. No Access

    Protocol

    Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides

    Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including αA,B-crystallin polypeptides, is characterized by a conserved C...

    André-Patrick Arrigo in Chaperones (2018)

  10. No Access

    Protocol

    Modification of Selenoprotein mRNAs by Cap Tri-methylation

    Several selenoprotein mRNAs undergo 5′ cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m3 2,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunop...

    Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang in Selenoproteins (2018)

  11. No Access

    Protocol

    Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies

    Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formula...

    Flávia Sousa, Pedro Fonte, Andreia Cruz in Recombinant Glycoprotein Production (2018)

  12. No Access

    Protocol

    Quantitation of ER Structure and Function

    The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin ...

    Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara in The Plant Endoplasmic Reticulum (2018)

  13. No Access

    Protocol

    Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level

    Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we...

    Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, Stefan Hohmann in Glucose Transport (2018)

  14. No Access

    Protocol

    Isolation and Characterization of Endogenous RNPs from Brain Tissues

    Identification of physiological target RNAs and protein interactors bound to RNA-binding proteins is a key prerequisite to understand the underlying mechanisms of posttranscriptional expression control and RNA...

    Rico Schieweck, Foong yee Ang, Renate Fritzsche, Michael A. Kiebler in RNA Detection (2018)

  15. No Access

    Protocol

    A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics

    Traditional drug susceptibility methods can take several days or weeks of incubation between drug exposure and confirmation of sensitivity or resistance. In addition, these methods do not capture information a...

    Charlotte L. Hendon-Dunn, Stephen R. Thomas in Antibiotic Resistance Protocols (2018)

  16. No Access

    Protocol

    Optocapacitance Allows for Photostimulation of Neurons without Requiring Genetic Modification

    Optocapacitance is a novel technique that combines much of the power of optogenetics without requiring any transfection or genome modification in the organism or tissue of interest. It functions via the same p...

    Joao L. Carvalho-de-Souza, Jeremy S. Treger in Use of Nanoparticles in Neuroscience (2018)

  17. No Access

    Protocol

    Differential Proteomics Based on 2D-Difference In-Gel Electrophoresis and Tandem Mass Spectrometry for the Elucidation of Biological Processes in Antibiotic-Producer Bacterial Strains

    Proteomics based on 2D-Difference In Gel Electrophoresis (2D-DIGE) coupled with mass spectrometry (MS) procedures can be considered a “gold standard” to determine quantitatively and comparatively protein abund...

    Giuseppe Gallo, Andrea Scaloni in Metabolic Network Reconstruction and Modeling (2018)

  18. No Access

    Protocol

    Optogenetics in Stem Cell Research: Focus on the Central Nervous System

    Stem cell-based therapies of CNS disorders represent a promising approach in translational and regenerative medicine. Stem cell-based tissue replacement and regeneration would, for the first time, offer a caus...

    Johannes Boltze, Albrecht Stroh in Optogenetics: A Roadmap (2018)

  19. No Access

    Protocol

    A Method for Testing Random Spatial Models on Nuclear Object Distributions

    The cell nucleus is a structurally complex and dynamic organelle ensuring key biological functions. Complex relationships between nuclear structure and functions require a better understanding of the three-dim...

    Javier Arpòn, Valérie Gaudin, Philippe Andrey in Plant Chromatin Dynamics (2018)

  20. No Access

    Protocol

    Recreating Intestinal Peristalsis in the Petri Dish

    Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic ref...

    Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez in Neurotrophic Factors (2018)

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