Abstract
In this chapter an introduction to the heavy-atom data bank is provided and strategies are reviewed that can be adopted in the preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering. Topics covered include: heavy-atom derivatives and their complexes; amino acids as ligands; the protein chemistry of heavy-atom reagents; metal-ion replacement in metalloproteins; analogues of amino acids; and the use of the heavy-atom data bank to select derivatives. This chapter is also available as HTML from the International Tables Online site hosted by the IUCr.
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© 2006 International Union of Crystallography
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Carvin, D., Islam, S.A., Sternberg, M.J.E., Blundell, T.L. (2006). The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering. In: Rossmann, M.G., Arnold, E. (eds) International Tables for Crystallography Volume F: Crystallography of biological macromolecules. International Tables for Crystallography, vol F. Springer, Dordrecht. https://doi.org/10.1107/97809553602060000679
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DOI: https://doi.org/10.1107/97809553602060000679
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6857-1
Online ISBN: 978-1-4020-5416-7
eBook Packages: Springer Book Archive