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Single-Cell Impedance Flow Cytometry

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Handbook of Single Cell Technologies

Abstract

This chapter reviews recent technical developments in the field of single-cell electrical property characterization. Firstly, three well-established characterization approaches including patch clamping, electrorotation, and dielectrophoresis are discussed and compared. Then, key developments of impedance flow cytometry, which are capable of high-throughput quantifying single-cell electrical properties, are presented. In addition, key cellular electrical properties quantified by these techniques are summarized and discussed. In the end, future research opportunities with technical innovations are indicated.

Hongyan Liang, Huiwen Tan, and Deyong Chen are Co-First Authors

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Acknowledgment

The authors would like to acknowledge financial supports from the National Natural Science Foundation of China (Grant No. 61431019, 61671430), Chinese Academy of Sciences Key Project Targeting Cutting-Edge Scientific Problems (QYZDB-SSW-JSC011), Instrument Development Program, Youth Innovation Promotion Association and Interdisciplinary Innovation Team of Chinese Academy of Sciences, and Instrument Development Program of Beijing Municipal Science and Technology Commission (Z181100009518001).

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Authors and Affiliations

  1. State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, People’s Republic of China

    Hongyan Liang, Huiwen Tan, Deyong Chen, Junbo Wang & Jian Chen

  2. University of Chinese Academy of Sciences, Beijing, People’s Republic of China

    Hongyan Liang, Huiwen Tan, Deyong Chen, Junbo Wang & Jian Chen

  3. Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, Taiwan

    Min-Hsien Wu

Authors
  1. Hongyan Liang
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  2. Huiwen Tan
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  3. Deyong Chen
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  4. Junbo Wang
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  5. Jian Chen
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  6. Min-Hsien Wu
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Corresponding authors

Correspondence to Junbo Wang , Jian Chen or Min-Hsien Wu .

Editor information

Editors and Affiliations

  1. Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India

    Tuhin Subhra Santra

  2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan

    Fan-Gang Tseng

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Liang, H., Tan, H., Chen, D., Wang, J., Chen, J., Wu, MH. (2019). Single-Cell Impedance Flow Cytometry. In: Santra, T., Tseng, FG. (eds) Handbook of Single Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-4857-9_7-1

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  • DOI: https://doi.org/10.1007/978-981-10-4857-9_7-1

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  • Print ISBN: 978-981-10-4857-9

  • Online ISBN: 978-981-10-4857-9

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