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Screening of Antigen-Specific Antibody-Secreting Cells

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Handbook of Single Cell Technologies

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Abstract

High-throughput screening of antigen-specific antibody-secreting cells (ASC) is an essential step in the discovery of monoclonal antibodies (mAb) which are the key candidates for therapeutic agents. To produce specific mAbs, various procedures have been developed including phage display library construction, production of stable hybridoma, or Epstein-Barr virus-immortalized human ASCs. However, the selection of antigen-specific ASCs remains as the bottleneck of these already labor-intensive processes. The screening technology must be (1) highly specific to the target antigen, (2) sensitive for the detection of low-volume secretions from a single live cell, (3) nondestructive to allow further processing of the cells, and (4) high throughput. Conventionally, B cell ELISpot is widely used to identify ASCs due to its high sensitivity and selectivity. However, multiplexing and cell recovery are difficult to achieve. Recently, advances in microengraving and nanowell-based technologies enabled highly multiplexed single-cell secretion detections. Droplet platforms have also emerged as a promising tool to screen single ASCs in a high-throughput manner. In this chapter, these technologies will be discussed in terms of their merits and drawbacks, as well as their suitability in screening antigen-specific ASCs.

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Authors and Affiliations

  1. Singapore-MIT Alliance for Research and Technology, Singapore, Singapore

    Myat Noe Hsu

  2. Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore

    Zirui Matthew Tay

  3. Department of Biomedical Engineering, National University of Singapore, Singapore, Singapore

    Weikang Nicholas Lin & Shih-Chung Wei

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  1. Myat Noe Hsu
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  2. Zirui Matthew Tay
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  4. Shih-Chung Wei
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Correspondence to Myat Noe Hsu .

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Editors and Affiliations

  1. Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India

    Tuhin Subhra Santra

  2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan

    Fan-Gang Tseng

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Hsu, M.N., Tay, Z.M., Lin, W.N., Wei, SC. (2020). Screening of Antigen-Specific Antibody-Secreting Cells. In: Santra, T., Tseng, FG. (eds) Handbook of Single Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-4857-9_27-1

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