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Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture

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Handbook of Single Cell Technologies

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Abstract

The clonal culture of single cells is widely used for studying cell dynamics and heterogeneity in vitro and producing monoclonal cell populations. Using traditional culture systems such as well plates to perform single cell culture experiments is of low efficiency because the Poisson distribution limits the available highest single cell event probability. Here we describe high-efficiency single cell isolation and long-term culture technique, which is based on using a microfluidic chip with a dual-well microchannel design. The utility of this technique is demonstrated with the isolation and culture of cancer and stem cells.

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Author notes

  1. The authors have equal contribution to the chapter.

Authors and Affiliations

  1. Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, Taiwan

    Chuan-Feng Yeh, Hao-Chen Chang & Chia-Hsien Hsu

  2. Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan

    Chuan-Feng Yeh & Chia-Hsien Hsu

  3. Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung, Taiwan

    Hao-Chen Chang & Chia-Hsien Hsu

Authors
  1. Chuan-Feng Yeh
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  2. Hao-Chen Chang
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  3. Chia-Hsien Hsu
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Corresponding author

Correspondence to Chia-Hsien Hsu .

Editor information

Editors and Affiliations

  1. Department of Engineering Design, Indian Institute of Technology Madras, Chennai, India

    Tuhin Subhra Santra

  2. Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan

    Fan-Gang Tseng

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Yeh, CF., Chang, HC., Hsu, CH. (2020). Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture. In: Santra, T., Tseng, FG. (eds) Handbook of Single Cell Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-10-4857-9_26-1

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  • DOI: https://doi.org/10.1007/978-981-10-4857-9_26-1

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-10-4857-9

  • Online ISBN: 978-981-10-4857-9

  • eBook Packages: Springer Reference EngineeringReference Module Computer Science and Engineering

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