Definition
The lipid extraction method by Bligh and Dyer is one of the standard procedures to isolate total lipid fractions from biological matrices based on a solvent system consisting of chloroform/methanol/water 2:2:1.8 (v/v/v).
Background
The extraction method by E.G. Bligh and W.J. Dyer was introduced in 1959 in search of an efficient and mild procedure to study lipid decomposition in frozen fish (Bligh and Dyer 1959). The basic principle for the extraction is based on Folch’s method (Folch-Pi et al. 1957) using less amounts of chloroform and methanol for the primary extraction step. A biological sample (e.g., tissue, blood, plasma) is homogenized in a monophasic chloroform/methanol/water mixture. Afterward a phase separation is induced by addition of chloroform and water. The same physicochemical principles for the distribution of chemical components between two nonmiscible solvents apply concerning Nernst’s partition law and diffusion as discussed in Folch’s method (Liquid...
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Abbreviations
- ESI-MS/MS:
-
Electrospray ionization tandem mass spectrometry
- GC:
-
Gas chromatography
- GC-MS:
-
Gas chromatography mass spectrometry
- HCl:
-
Hydrochloric acid
- HPLC:
-
High-performance liquid chromatography
- LC-MS:
-
Liquid chromatography mass spectrometry
References
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Folch-Pi J, Lees M, Stanley GHS. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957;226:497–509.
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Sündermann, A., Eggers, L.F., Schwudke, D. (2016). Liquid Extraction: Bligh and Dyer. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_88-1
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DOI: https://doi.org/10.1007/978-94-007-7864-1_88-1
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