Skip to main content

Electrospray Ionization Mass Spectrometry of Phospholipids

  • Living reference work entry
  • First Online:
Book cover Encyclopedia of Lipidomics

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Institutional subscriptions

References

  • Bandu R, Mok HJ, Kim KP. Phospholipids as cancer biomarkers: mass spectrometry-based analysis. Mass Spectrom Rev. 2018;37:107–38.

    Article  CAS  PubMed  Google Scholar 

  • Bielawska K, Dziakowska I, Roszkowska-Jakimiec W. Chromatographic determination of fatty acids in biological material. Toxicol Mech Methods. 2010;20:526–37.

    Article  CAS  PubMed  Google Scholar 

  • Brügger B. Lipidomics: analysis of the lipid composition of cells and subcellular organelles by electrospray ionization mass spectrometry. Annu Rev Biochem. 2014;83:79–98.

    Article  PubMed  CAS  Google Scholar 

  • Bruins AP. Mechanistic aspects of electrospray ionization. J Chromatogr A. 1998;794:345–57.

    Article  CAS  Google Scholar 

  • Byrdwell WC. Atmospheric pressure chemical ionization mass spectrometry for analysis of lipids. Lipids. 2001;36:327–46.

    Article  CAS  PubMed  Google Scholar 

  • Domingues MR, Reis A, Domingues P. Mass spectrometry analysis of oxidized phospholipids. Chem Phys Lipids. 2008;156:1–12.

    Article  CAS  PubMed  Google Scholar 

  • Eibisch M, Fuchs B, Schiller J, Süß R, Teuber K. Analysis of phospholipid mixtures from biological tissues by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS): a laboratory experiment. J Chem Educat. 2011;88:503–7.

    Article  CAS  Google Scholar 

  • Ellis SR, Brown SH, In Het Panhuis M, Blanksby SJ, Mitchell TW. Surface analysis of lipids by mass spectrometry: more than just imaging. Prog Lipid Res. 2013;52:329–53.

    Article  CAS  PubMed  Google Scholar 

  • Engel KM, Schiller J. A comparison of PC oxidation products as detected by MALDI-TOF and ESI-IT mass spectrometry. Chem Phys Lipids. 2017;203:33–45.

    Article  CAS  PubMed  Google Scholar 

  • Engel KM, Griesinger H, Schulz M, Schiller J. Normal-phase versus reversed-phase thin-layer chromatography (TLC) to monitor oxidized phosphatidylcholines by TLC/mass spectrometry. Rapid Commun Mass Spectrom. 2019;33:60–5.

    Article  CAS  PubMed  Google Scholar 

  • Fuchs B, Schiller J. Mass spectrometry of biological molecules. In: Encyclopedia of analytical chemistry. John Wiley & Sons Ltd., Chichester; 2016.

    Google Scholar 

  • Fuchs B, Süss R, Schiller J. An update of MALDI-TOF mass spectrometry in lipid research. Prog Lipid Res. 2010;49:450–75.

    Article  CAS  PubMed  Google Scholar 

  • Fuchs B, Süss R, Teuber K, Eibisch M, Schiller J. Lipid analysis by thin-layer chromatography – a review of the current state. J Chromatogr A. 2011;1218:2754–74.

    Article  CAS  PubMed  Google Scholar 

  • Furey A, Moriarty M, Bane V, Kinsella B, Lehane M. Ion suppression; a critical review on causes, evaluation, prevention and applications. Talanta. 2013;115:104–22.

    Article  CAS  PubMed  Google Scholar 

  • Giles C, Takechi R, Lam V, Dhaliwal SS, Mamo JCL. Contemporary lipidomic analytics: opportunities and pitfalls. Prog Lipid Res. 2018;71:86–100.

    Article  CAS  PubMed  Google Scholar 

  • Gode D, Volmer, DA. Lipid imaging by mass spectrometry – a review. Analyst. 2013;138:1289–315.

    Article  CAS  PubMed  Google Scholar 

  • Griesinger H, Fuchs B, Süß R, Matheis K, Schulz M, Schiller J. Stationary phase thickness determines the quality of thin-layer chromatography/matrix-assisted laser desorption and ionization mass spectra of lipids. Anal Biochem. 2014;451:45–7.

    Article  CAS  PubMed  Google Scholar 

  • Groessl M, Graf S, Knochenmuss R. High resolution ion mobility-mass spectrometry for separation and identification of isomeric lipids. Analyst. 2015;140:6904–11.

    Article  CAS  PubMed  Google Scholar 

  • Gros M, Borros S, Amabilino DB, Veciana J, Folch I. Characterization of the vulcanization products of squalene by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: model studies on the vulcanization of natural rubber. J Mass Spectrom. 2001;36:294–300.

    Article  CAS  Google Scholar 

  • Gross JH. Mass spectrometry – a textbook. 2nd ed. Heidelberg: Springer; 2011.

    Book  Google Scholar 

  • Hofmann T, Schmidt C. Instrument response of phosphatidylglycerol lipids with varying fatty acyl chain length in nano-ESI shotgun experiments. Chem Phys Lipids. 2019;223:104782.

    Article  CAS  PubMed  Google Scholar 

  • Hsu FF, Lodhi IJ, Turk J, Semenkovich CF. Structural distinction of diacyl-, alkylacyl, and alk-1-enylacyl glycerophosphocholines as [M – 15] ions by multiple-stage linear ion-trap mass spectrometry with electrospray ionization. J Am Soc Mass Spectrom. 2014;25:1412–20.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hyötyläinen T, Orešič M. Systems biology strategies to study lipidomes in health and disease. Prog Lipid Res. 2014;55:43–60.

    Article  PubMed  CAS  Google Scholar 

  • Jaskolla TW, Karas M. Compelling evidence for Lucky Survivor and gas phase protonation: the unified MALDI analyte protonation mechanism. J Am Soc Mass Spectrom. 2011;22:976–88.

    Article  CAS  PubMed  Google Scholar 

  • Kayganich KA, Murphy RC. Fast atom bombardment tandem mass spectrometric identification of diacyl, alkylacyl, and alk-1-enylacyl molecular species of glycerophosphoethanolamine in human polymorphonuclear leukocytes. Anal Chem. 1992;64:2965–71.

    Article  CAS  PubMed  Google Scholar 

  • Kilár A, Dörnyei Á, Kocsis B. Structural characterization of bacterial lipopolysaccharides with mass spectrometry and on- and off-line separation techniques. Mass Spectrom Rev. 2013;32:90–117.

    Article  PubMed  CAS  Google Scholar 

  • Koivusalo M, Haimi P, Heikinheimo L, Kostiainen R, Somerharju P. Quantitative determination of phospholipid compositions by ESI-MS: effects of acyl chain length, unsaturation, and lipid concentration on instrument response. J Lipid Res. 2001;42:663–72.

    PubMed  Google Scholar 

  • Lam SM, Shui G. Lipidomics as a principal tool for advancing biomedical research. J Genet Genomics. 2013;40:375–90.

    Article  CAS  PubMed  Google Scholar 

  • Li Z, Agellon LB, Allen TM, Umeda M, Jewell L, Mason A, Vance DE. The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell Metab. 2006;3:321–31.

    Article  CAS  PubMed  Google Scholar 

  • Li X, He Q, Hou H, Zhang S, Zhang X, Zhang Y, Wang X, Han L, Liu K. Targeted lipidomics profiling of marine phospholipids from different resources by UPLC-Q-Exactive Orbitrap/MS approach. J Chromatogr B. 2018;1096:107–12.

    Article  CAS  Google Scholar 

  • Liebisch G, Vizcaíno JA, Köfeler H, Trötzmüller M, Griffiths WJ, Schmitz G, Spener F, Wakelam MJ. Shorthand notation for lipid structures derived from mass spectrometry. J Lipid Res. 2013;54:1523–30.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu S, Sjövall J, Griffiths WJ. Neurosteroids in rat brain: extraction, isolation, and analysis by nanoscale liquid chromatography-electrospray mass spectrometry. Anal Chem. 2003;75:5835–46.

    Article  CAS  PubMed  Google Scholar 

  • Nimptsch A, Fuchs B, Süß R, Zschörnig K, Jakop U, Göritz F, Schiller J, Müller K. A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry. Anal Bioanal Chem. 2013;405:6675–82.

    Article  CAS  PubMed  Google Scholar 

  • Pham HT, Maccarone AT, Thomas MC, Campbell JL, Mitchell TW, Blanksby SJ. Structural characterization of glycerophospholipids by combinations of ozone- and collision-induced dissociation mass spectrometry: the next step towards “top-down” lipidomics. Analyst. 2014;139:204–14.

    Article  CAS  PubMed  Google Scholar 

  • Pulfer M, Murphy RC. Electrospray mass spectrometry of phospholipids. Mass Spectrom Rev. 2003;22:332–64.

    Article  CAS  PubMed  Google Scholar 

  • Sales S, Knittelfelder O, Shevchenko A. Lipidomics of human blood plasma by high-resolution shotgun mass spectrometry. Methods Mol Biol. 2017;1619:203–12.

    Article  CAS  PubMed  Google Scholar 

  • Sethi S, Brietzke E. Recent advances in lipidomics: analytical and clinical perspectives. Prostaglandins Other Lipid Mediat. 2017;128–129:8–16.

    Article  PubMed  CAS  Google Scholar 

  • Tuzimski T, Sherma J. Encyclopedia of lipidomics. Thin-layer chromatography and mass spectrometry for the analysis of lipids. Dordrecht: Springer; 2016.

    Google Scholar 

  • Vasquez AM, Mouchlis VD, Dennis EA. Review of four major distinct types of human phospholipase A2. Adv Biol Regul. 2018;67:212–8.

    Article  CAS  PubMed  Google Scholar 

  • Wang C, Wang M, Han X. Applications of mass spectrometry for cellular lipid analysis. Mol Biosyst. 2015;11:698–713.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wang M, Wang C, Han RH, Han X. Novel advances in shotgun lipidomics for biology and medicine. Prog Lipid Res. 2016;61:83–108.

    Article  CAS  PubMed  Google Scholar 

  • Wang M, Wang C, Han X. Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why? Mass Spectrom Rev. 2017;36:693–714.

    Article  CAS  PubMed  Google Scholar 

  • Wang J, Wang C, Han X. Tutorial on lipidomics. Anal Chim Acta. 2019;1061:28–41.

    Article  CAS  PubMed  Google Scholar 

  • Wilm M. Principles of electrospray ionization. Mol Cell Proteomics. 2011;10:M111.009407.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wozny K, Lehmann WD, Wozny M, Akbulut BS, Brügger B. A method for the quantitative determination of glycerophospholipid regioisomers by UPLC-ESI-MS/MS. Anal Bioanal Chem. 2019;411:915–24.

    Article  CAS  PubMed  Google Scholar 

  • Yang K, Han X. Lipidomics: techniques, applications, and outcomes related to biomedical sciences. Trends Biochem Sci. 2016;41:954–69.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao YY, Cheng XL, Lin RC, Wei F. Lipidomics applications for disease biomarker discovery in mammal models. Biomark Med. 2015;9:153–68.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kathrin M. Engel .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature B.V.

About this entry

Check for updates. Verify currency and authenticity via CrossMark

Cite this entry

Engel, K.M., Popkova, Y. (2019). Electrospray Ionization Mass Spectrometry of Phospholipids. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_198-1

Download citation

  • DOI: https://doi.org/10.1007/978-94-007-7864-1_198-1

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-7864-1

  • Online ISBN: 978-94-007-7864-1

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

Publish with us

Policies and ethics