Ameloblastin as Biomarker of Bone

  • Juliane IsaacEmail author
  • Guilhem Lignon
  • Jaime Jacques
  • Ariane Berdal
Reference work entry
Part of the Biomarkers in Disease: Methods, Discoveries and Applications book series (BDMDA)


Regional bone diversity has major public health implications. This is exemplified by the tissue incompatibility problems associated with bone ectopic autografts or the puzzling jaw osteonecrosis induced by antiresorptive agents that are otherwise effective in treating long-bone osteoporosis or metastatic resorptive lesions. Identifying bone site-specific biomarkers is therefore essential, firstly to determine why or how bone cell phenotypes vary depending on the anatomical site and secondly to implement new bone site-specific therapeutics. The present chapter summarizes findings on site-specific bone cell profiles and highlights ameloblastin (AMBN) as an exemplary peptide for jaw bone site-specificity. AMBN was originally discovered in tooth enamel matrix, extracts of which have been successfully applied clinically for regeneration of mineralized tissue. AMBN has also been detected outside the enamel in both mineralized and nonmineralized tissues. In bone, functional studies have demonstrated crucial functions of AMBN in the control of bone balance, notably processes associated with a high bone remodeling rate. In contrast to appendicular and axial bones, jaw bones are highly affected by AMBN. For example, AMBN participates in the physiological control of alveolar bone integrity in response to tooth-associated biomechanical stimulation. Based on these observations, AMBN-based treatments have promising clinical potential for craniofacial tissue repair and more specifically for alveolar bone regeneration.


Ameloblastin Enamel Osteoblast Bone remodeling Alveolar bone Site-specificity Bone regeneration 

List of Abbreviations


Adipose-derived adult stem cell


Alkaline phosphatase






Bone marrow-derived monocyte/macrophage cell


Bone morphogenetic protein


Bone marrow stromal cell




BP-related osteonecrosis of the jaw


Dental follicle


Demineralized freeze dried bone allograft


Extracellular matrix


Enamel matrix derivative


Enamel protein


Epithelial rest of Malassez


Embryonic stem


Freeze dried bone allograft


Hertwig’s epithelial root sheath


Human oral mucosa stem cell






In situ hybridization


Intrinsically unstructured protein


Kallikrein 4


Laser capture microdissection


Lock nucleic acid


Leucine rich amelogenin peptide




Mesenchymal stem cell


Muscle segment homeobox


Northern blot


Natural bovine mineral


Normal human osteoblast








Peripheral blood mononuclear cell


Periodontal ligament


Parathyroid hormone


Receptor activator of nuclear factor kappa b


Receptor activator of nuclear factor kappa b ligand


Ribonucleic acid


Reverse transcription polymerase chain reaction


Reverse transcription quantitative polymerase chain reaction


Squamous cell carcinoma


Secretory calcium-binding phosphoprotein




Small integrin binding ligand N-linked glycoprotein


Secreted protein, acidic, cysteine-rich


Signal transducer and activator of transcription


Western blot


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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Juliane Isaac
    • 1
    • 2
    • 3
    • 4
    • 5
    • 6
    Email author
  • Guilhem Lignon
    • 1
    • 2
    • 3
    • 4
  • Jaime Jacques
    • 1
    • 7
  • Ariane Berdal
    • 1
    • 2
    • 3
    • 4
    • 5
    • 8
  1. 1.Laboratory of Molecular Oral Pathophysiology, INSERM UMRS 1138, Team BerdalCordeliers Research CenterParisFrance
  2. 2.Pierre and Marie Curie University (Paris 6)ParisFrance
  3. 3.Paris Descartes University (Paris 5)ParisFrance
  4. 4.Paris Diderot University (Paris 7)ParisFrance
  5. 5.UFR d’OdontologieParis Diderot University (Paris 7)ParisFrance
  6. 6.Laboratory of Morphogenesis Molecular Genetics, Department of Developmental and Stem Cell Biology, CNRS URA 2578Institut PasteurParisFrance
  7. 7.Periodontology Unit, Department of Stomatology-Faculty of Health ScienceUniversity of TalcaTalcaChile
  8. 8.Reference Center for Buccal and Facial Malformations CRMR MAFACEHopital Rotshild-APHPParisFrance

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