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Shotgun Approaches for Venom Analysis

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Venom Genomics and Proteomics

Part of the book series: Toxinology ((TOXI))

Abstract

Shotgun proteomics relies on the identification, quantification, and characterization of proteins in complex samples. Recent advances in instrumentation allow for sensitive and comprehensive shotgun protein analysis in a high-throughput manner. Combination of shotgun techniques to novel analytical strategies opens interesting possibilities for the implementation of new approaches and methodologies in the frontiers of venom biology. Examples are (i) identification of proteins in low abundance, using combinatorial ligand peptide libraries; (ii) relative and absolute protein quantitation ; and (iii) identification of posttranslational modifications. The full potential of shotgun analysis in venomics is yet to be explored. Some of the pioneer works in the field will be reviewed.

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Authors and Affiliations

  1. Proteomics Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Av Athos da Silveira Ramos 149 - CT - A 542 Cidade Universitária, 21949-909, Rio de Janeiro, RJ, Brazil

    Rafael Donadelli Melani, Livia Goto-Silva, Fábio César Sousa Nogueira, Magno Junqueira & Gilberto Barbosa Domont

Authors
  1. Rafael Donadelli Melani
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  2. Livia Goto-Silva
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  3. Fábio César Sousa Nogueira
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  4. Magno Junqueira
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  5. Gilberto Barbosa Domont
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Corresponding author

Correspondence to Gilberto Barbosa Domont .

Editor information

Editors and Affiliations

  1. Venom and Toxin Research Programme, Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    P. Gopalakrishnakone

  2. Structural and Functional Venomics Lab, Instituto de Biomedicina de Valencia (CSIC), Valencia, Spain

    Juan J. Calvete

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Melani, R.D., Goto-Silva, L., Nogueira, F.C.S., Junqueira, M., Domont, G.B. (2016). Shotgun Approaches for Venom Analysis. In: Gopalakrishnakone, P., Calvete, J. (eds) Venom Genomics and Proteomics. Toxinology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6416-3_26

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