Abstract
We here describe the three-dimensional optical tracking method, which is realized with a simple optical component, a quadrangular wedge prism. Additional two lenses located between a conventional optical microscope and a camera enable to track single particles in 3-D. Because of the simplicity of its rationale and construction, any laboratory equipped with 2-D tracking method, under either fluorescence, phase-contrast, bright-field or dark-field illumination, can adopt our method with the same analysis procedure and thus the same precision. Applications to a molecular motor, kinesin-microtubule system, and optical trapping, are also demonstrated, verifying the advantage of our approach to assess the movement of tiny objects, with the size ranging from ten nanometers to a few microns, in an aqueous solution.
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References
Ashkin A (1970) Acceleration and trapping of particles by radiation pressure. Phys Rev Lett 24:156–159
Ashkin A (1984) Stable radiation-pressure particle traps using alternating light beams. Opt Lett 9:454
Ashkin A, Dziedzic JM (1985) Observation of radiation-pressure trapping of particles by alternating light beams. Phys Rev Lett 54:1245–1248
Ashkin A, Dziedzic JM, Bjorkholm JE, Chu S (1986) Observation of a single-beam gradient force optical trap for dielectric particles. Opt Lett 11:288–290
Molloy JE, Padgett MJ (2002) Lights, action: optical tweezers. Contemp Phys 55:241–258
Neuman KC, Abbondanzieri EA, Block SM (2005) Measurement of the effective focal shift in an optical trap. Opt Lett 30:1318–1320
Moffitt JR, Chemla YR, Smith SB, Bustamante C (2008) Recent advances in optical tweezers. Annu Rev Biochem 77:205–228
Marago OM, Jones PH, Gucciardi PG, Volpe G, Ferrari AC (2013) Optical trapping and manipulation of nanostructures. Nat Nanotechnol 8:807–819
Yajima J, Mizutani K, Nishizaka T (2008) A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking. Nat Struct Mol Biol 15:1119–1121
Deschout H et al (2014) Precisely and accurately localizing single emitters in fluorescence microscopy. Nat Methods 11:253–266
Tsuji T et al (2011) Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells. Biochem Biophys Res Commun 405:638–643
Yildiz A, Tomishige M, Vale RD, Selvin PR (2004) Kinesin walks hand-over-hand. Science 303:676–678
Yajima J, Cross RA (2005) A torque component in the kinesin-1 power stroke. Nat Chem Biol 1:338–341
Miyata M, Ryu WS, Berg HC (2002) Force and velocity of mycoplasma mobile gliding. J Bacteriol 184:1827–1831
Nishizaka T, Miyata H, Yoshikawa H, Ishiwata S, Kinosita K Jr (1995) Unbinding force of a single motor molecule of muscle measured using optical tweezers. Nature 377:251–254
Acknowledgments
This study was supported in part by the Funding Program for Next Generation World-Leading Researchers Grant LR033 (to T. N.) from the Japan Society for the Promotion of Science and by Grants-in-Aid for Scientific Research on Innovative Areas “Harmonized Supramolecular Motility Machinery and Its Diversity” (Grant 24117002 to T. N.), “Fluctuation & Structure” (Grant 26103527 to T. N.) and “Cilia & Centrosomes” (Grant 87003306 to T.N.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Katoh, T.A., Fujimura, S., Nishizaka, T. (2017). 3-D Single Particle Tracking Using Dual Images Divided by Prism: Method and Application to Optical Trapping. In: Ho, AP., Kim, D., Somekh, M. (eds) Handbook of Photonics for Biomedical Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5052-4_2
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DOI: https://doi.org/10.1007/978-94-007-5052-4_2
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