Abstract
For evaluating the weak interaction between sugar chains and proteins, regular glycol-array technology might not be effective because of the many washing steps between the binding and visualization of the bound proteins. It is crucial to eliminate the washing steps and to visualize the binding interaction at the real time. Using our original array-type sugar chain-immobilized chip (named array-type Sugar Chip, A-SC) and SPR imaging technology, an easy and conventional analytical method was developed for such a weak interaction. This method was applied to evaluate the sugar chain binding property of novel proteins, as well as of various viruses. Here, we focus on the glycosaminoglycan (GAG)-immobilized A-SC and report the results of analysis for the GAG structures to which human herpesviruses (HHVs) bind at the molecular level.
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References
Christopher D et al (2010) Expanding the role of 3-O sulfated heparan sulfate in herpes simplex virus type-1 entry. Virology 397:389–398
Johnson RM, Spear PG (1989) Herpes simplex virus glycoprotein D mediates interference with herpes simplex virus infection. J Virol 63(2):819–827
Lycke E et al (1991) Binding of herpes simplex virus to cellular heparan sulphate, an initial step in the adsorption process. J Gen Virol 72:1131–1137
Nakamura-Tsuruta S et al (2008) One-step purification of lectins from banana pulp using sugar-immobilized gold nano-particles. J Biochem 143:833–839
Poletti LF et al (1997) Structural aspects of heparin responsible for interactions with Von Willebrand Factor. Arterioscler Thromb Vasc Biol 17(5):925–931
Suda Y et al (1996) Synthesis and biological activity of a model disaccharide containing a key unit in heparin for binding to platelets. Tetrahedron Lett 37:1053
Suda Y et al (2006) Immobilization and clustering of structurally defined oligosaccharides for sugar chips: an improved method for surface plasmon resonance analysis of protein carbohydrate interactions. Bioconjug Chem 17:1125–1135
Suda Y et al (2013) Discrimination of influenza virus strains and super high sensitive detection of viruses using sugar chip and sugar-chain immobilized gold nanoparticles. In: Scholz C (ed) Tailored polymer architectures for pharmaceutical and biomedical applications. ACS symposium series, vol 1135. pp 331–350
Wakao M et al (2008) Sugar chips immobilized with synthetic sulfated disaccharides of heparin/heparan sulfate partial structure. Bioorg Med Chem Lett 18:2499–2504
WuDunn D, Spear PG (1989) Initial interaction of herpes simplex virus with cells is binding to heparan sulfate. J Virol 63:52–58
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Suda, Y., Zhang, X., Nakamura-Tsuruta, S., Sobel, M., Okuno, T., Wakao, M. (2015). Conventional and Easy Analysis of Virus-Binding GAG Structure Using Array-Type Sugar Chip. In: Taniguchi, N., Endo, T., Hart, G., Seeberger, P., Wong, CH. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54841-6_14
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DOI: https://doi.org/10.1007/978-4-431-54841-6_14
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Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-54840-9
Online ISBN: 978-4-431-54841-6
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