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Glycosphingolipid Analysis by Liquid Chromatography-Mass Spectrometry

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Glycoscience: Biology and Medicine

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Abstract

Glycosphingolipids (GSLs) are an essential component of the microdomains that provide suitable microenvironments for membrane molecules and support their functions. The characterization of GSLs is the first step in the functional analysis of GSLs. The structures of the glycan chains and lipid parts of GSLs can be characterized by a sensitive method using liquid chromatography–mass spectrometry (LC-MS) and mixtures of GSLs. GSLs are minor components of the total lipids of tissues and cells. Therefore, sample preparation methods suitable for LC-MS analysis are critical. This chapter describes a protocol for the ganglioside analysis of mouse cerebellum, which consists of lipid extraction, solvent partition, and LC-MS analysis with C30 reversed-phase chromatography.

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Author information

Authors and Affiliations

  1. Institute of Glycoscience, Tokai University, Kanagawa, Japan

    Akemi Suzuki & Kunihiro Sano

  2. School of Integrative and Global Majors, Ph.D. Program in Human Biology, University of Tsukuba, Ibaraki, Japan

    Minoru Suzuki

  3. Shimadzu Corporation, Kyoto, Japan

    Yoshikatsu Umemura & Hideshi Fujiwake

Authors
  1. Akemi Suzuki
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  2. Kunihiro Sano
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  3. Minoru Suzuki
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  4. Yoshikatsu Umemura
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  5. Hideshi Fujiwake
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Corresponding author

Correspondence to Akemi Suzuki .

Editor information

Editors and Affiliations

  1. Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama, Japan

    Naoyuki Taniguchi

  2. Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, Tokyo, Japan

    Tamao Endo

  3. Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Gerald W. Hart

  4. Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

    Peter H. Seeberger

  5. Academica Sinica, NanKang, T´ai-pei, Taiwan

    Chi-Huey Wong

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© 2015 Springer Japan

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Suzuki, A., Sano, K., Suzuki, M., Umemura, Y., Fujiwake, H. (2015). Glycosphingolipid Analysis by Liquid Chromatography-Mass Spectrometry. In: Taniguchi, N., Endo, T., Hart, G., Seeberger, P., Wong, CH. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54841-6_12

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