GPI Mannose Extension (PIGM, PIGV, PIGB, PIGZ)

Reference work entry


Glycosylphosphatidylinositol (GPI)-anchor is a major form of posttranslational modification that serves as a membrane anchor of many cell surface proteins. In human cells, more than 150 different proteins are GPI-anchored. The core backbone of the GPI-anchors contains three mannoses (Man1, Man2, and Man3), each linked in different bonds. In some cases, the fourth mannose (Man4) is added to Man3 as a side branch (Fig. 107.1) (Kinoshita et al. 2008). The structure of GPI-anchor bearing four mannoses is EtNP6(Man4α1-2)Man3α1-2(EtNP6)Man2α1-6(EtNP2)Man1α1-4GlcNα1-6(2-acyl)myo-inositol phospholipid (EtNP, ethanolaminephosphate; GlcN, glucosamine). All four mannoses are donated by dolichol-phosphate-mannose (Dol-P-Man). Four Dol-P-Man-dependent α-mannosyltransferases are therefore involved in biosynthesis of the GPI-anchors, i.e., GPI-Man transferase (GPI-MT)-I, GPI-MT-II, GPI-MT-III, and GPI-MT-IV for Man1, Man2, Man3, and Man4, respectively. All four enzymes are endoplasmic reticulum (ER)-resident membrane proteins belonging to a superfamily of glycosyltransferases that share several characteristics, such as the presence of multiple transmembrane domains (8–12) and the presence of catalytic (or putative catalytic) site in the luminal region between the first and the second transmembrane domains (Oriol et al. 2002; Liu and Mushegian 2003).


Chinese Hamster Ovary Cell Predict Transmembrane Domain Hepatic Vein Thrombosis Benzenesulfonyl Fluoride Hydrochloride Multiple Transmembrane Domain 
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Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Department of ImmunoregulationResearch Institute for Microbial Diseases, Osaka UniversitySuitaJapan
  2. 2.Department of Molecular GeneticsOsaka Medical Center for Cancer and Cardiovascular DiseasesOsakaJapan

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