Solute Carrier Family 35 (UDP-Galactose Transporter), Member A2 (SLC35A2)

Reference work entry


UDP-Gal transporter (SLC35A2) is a multiple-segment transmembrane protein of the Golgi apparatus that delivers UDP-Gal, synthesized in the cytosol, into the Golgi lumen to provide various Gal transferases with their substrate for the elongation of carbohydrate chains (Kawakita et al. 1998). Gal transferases, whose catalytic sites face the Golgi lumen, cannot add Gal residues to growing carbohydrate chains unless UDP-Gal is supplied through UDP-Gal transporter. Thus, mutant cultured cell lines defective in UDP-Gal transporter were shown to accumulate Glc-Cer instead of lactosylceramide (Taki et al. 1991), and truncated N-linked oligosaccharide chains terminated at GlcNAc (Hara et al. 1989a).


Solute Carrier Family Microsomal Vesicle Golgi Lumen Sugar Transporter Family cDNA Expression System 
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  1. Aoki K, Ishida N, Kawakita M (2003) Substrate recognition by nucleotide sugar transporters: further characterization of substrate recognition regions by analyses of UDP-galactose/CMP-sialic acid transporter chimeras and biochemical analysis of the substrate specificity of parental and chimeric transporters. J Biol Chem 278:22887–22893PubMedCrossRefGoogle Scholar
  2. Brandan E, Fleischer B (1982) Orientation and role of nucleosidediphosphatase and 5′-nucleotidase in Golgi vesicles from rat liver. Biochemistry 21:4640–4645PubMedCrossRefGoogle Scholar
  3. Csala M, Marcolongo P, Lizak B, Senesi S, Margittai E, Fulceri R, Magyar JE, Benedetti A, Banhegyi G (2007) Transport and transporters in the endoplasmic reticulum. Biochim Biophys Acta 1768:1325–1341PubMedCrossRefGoogle Scholar
  4. Dennis JW, Carver JP, Schachter H (1984) Asparagine-linked oligosaccharides in murine tumor cells: comparison of a WGA-resistant (WGAr) nonmetastatic mutant and a related WGA-sensitive (WGAs) metastatic line. J Cell Biol 99:1034–1044PubMedCrossRefGoogle Scholar
  5. Deutscher SL, Hirschberg CB (1986) Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes. J Biol Chem 261:96–100PubMedGoogle Scholar
  6. Eckhardt M, Gotza B, Gerardy-Schahn R (1999) Membrane topology of the mammalian CMP-sialic acid transporter. J Biol Chem 274:8779–8787PubMedCrossRefGoogle Scholar
  7. Hara T, Endo T, Furukawa K, Kawakita M, Kobata A (1989a) Elucidation of the phenotypic change on the surface of Had-1 cell, a mutant cell line of mouse FM3A carcinoma cells selected by resistance to Newcastle disease virus infection. J Biochem 106:236–247PubMedGoogle Scholar
  8. Hara T, Hattori S, Kawakita M (1989b) Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease virus receptors. J Virol 63:182–188PubMedCentralPubMedGoogle Scholar
  9. Hara T, Yamauchi M, Takahashi E, Hoshino M, Aoki K, Ayusawa D, Kawakita M (1993) The UDP-galactose translocator gene is mapped to band Xp11.23-p11.22 containing the Wiskott-Aldrich syndrome locus. Somat Cell Mol Gen 19:571–575CrossRefGoogle Scholar
  10. Ishida N, Ito M, Yoshioka S, Sun-Wada GH, Kawakita M (1998) Functional expression of human Golgi CMP-sialic acid transporter in the Golgi complex of a transporter-deficient Chinese hamster ovary cell mutant. J Biochem 124:171–178PubMedCrossRefGoogle Scholar
  11. Ishida N, Kawakita M (2004) Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). Pflügers Arch Eur J Physiol 447:768–775CrossRefGoogle Scholar
  12. Ishida N, Miura N, Yoshioka S, Kawakita M (1996) Molecular cloning and characterization of a novel isoform of the human UDP-galactose transporter, and of related complementary DNAs belonging to the nucleotide-sugar transporter gene family. J Biochem 120:1074–1078PubMedCrossRefGoogle Scholar
  13. Kannagi R (1997) Carbohydrate-mediated cell adhesion involved in hematogenous metastasis of cancer. Glycoconj J 14:577–584PubMedCrossRefGoogle Scholar
  14. Kawakita M, Ishida N, Miura N, Sun-Wada GH, Yoshioka S (1998) Nucleotide sugar transporters: elucidation of their molecular identity and its implication for future studies. J Biochem 123:777–785PubMedCrossRefGoogle Scholar
  15. Kuhn NJ, White A (1976) Evidence for specific transport of uridine diphosphate galactose across the Golgi membrane of rat mammary gland. Biochem J 154:243–244PubMedGoogle Scholar
  16. Kumamoto K, Goto Y, Sekikawa K, Takenoshita S, Ishida N, Kawakita M, Kannagi R (2001) Increased expression of UDP-galactose transporter messenger RNA in human colon cancer tissues and its implication in synthesis of Thomsen-Friedenreich antigen and sialyl Lewis A/X determinants. Cancer Res 61:4620–4627PubMedGoogle Scholar
  17. Liu L, Xu YX, Hirschberg CB (2010) The role of nucleotide sugar transporters in development of eukaryotes. Sem Cell Dev Biol 21:600–608CrossRefGoogle Scholar
  18. Maszczak-Seneczko D, Olczak T, Wunderlich L, Olczak M (2011) Comparative analysis of involvement of UGT1 and UGT2 splice variants of UDP-galactose transporter in glycosylation of macromolecules in MDCK and CHO cell lines. Glycoconj J 28:481–492PubMedCentralPubMedCrossRefGoogle Scholar
  19. Maszczak-Seneczko D, Sosicka P, Majkowski M, Olczak T, Olczak M (2012) UDP-N-acetylglucosamine transporter and UDP-galactose transporter form heterologous complexes in the Golgi membrane. FEBS Lett 586:4082–4087PubMedCrossRefGoogle Scholar
  20. Mayinger P, Meyer DI (1993) An ATP transporter is required for protein translocation into the yeast endoplasmic reticulum. EMBO J 12:659–666PubMedGoogle Scholar
  21. Milla ME, Clairmont CA, Hirschberg CB (1992) Reconstitution into proteoliposomes and partial purification of the Golgi apparatus membrane UDP-galactose, UDP-xylose, and UDP-glucuronic acid transport activities. J Biol Chem 267:103–107PubMedGoogle Scholar
  22. Milla ME, Hirschberg CB (1989) Reconstitution of Golgi vesicle CMP-sialic acid and adenosine 3′-phosphate 5′-phosphosulfate transport into proteoliposomes. Proc Natl Acad Sci USA 86:1786–1790PubMedCrossRefGoogle Scholar
  23. Miura N, Ishida N, Hoshino M, Yamauchi M, Hara T, Ayusawa D, Kawakita M (1996) Human UDP-galactose translocator: molecular cloning of a complementary DNA that complements the genetic defect of a mutant cell line deficient in UDP-galactose translocator. J Biochem 120:236–241PubMedCrossRefGoogle Scholar
  24. Muraoka M, Miki T, Ishida N, Hara T, Kawakita M (2007) Variety of nucleotide sugar transporters with respect to the interaction with nucleoside mono- and diphosphates. J Biol Chem 282:24615–24622PubMedCrossRefGoogle Scholar
  25. Ng BG, Buckingham KJ, Raymond K, Kircher M, Turner EH, He M, Smith JD, Eroshkin A, Szybowska M, Losfeld ME, Chong JX, Kozenko M, Li C, Patterson MC, Gilbert RD, Nickerson DA, Shendure J, Bamshad MJ, Freeze HH (2013) Mosaicism of the UDP-galactose transporter SLC35A2 causes a congenital disorder of glycosylation. Am J Hum Gen 92:632–636CrossRefGoogle Scholar
  26. Perez M, Hirschberg CB (1987) Transport of sugar nucleotides into the lumen of vesicles derived from rat liver rough endoplasmic reticulum and Golgi apparatus. Method Enzymol 138:709–715CrossRefGoogle Scholar
  27. Segawa H, Kawakita M, Ishida N (2002) Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose. Eur J Biochem 269:128–138PubMedCrossRefGoogle Scholar
  28. Segawa H, Soares RP, Kawakita M, Beverley SM, Turco SJ (2005) Reconstitution of GDP-mannose transport activity with purified Leishmania LPG2 protein in liposomes. J Biol Chem 280:2028–2035PubMedCrossRefGoogle Scholar
  29. Sun-Wada GH, Yoshioka S, Ishida N, Kawakita M (1998) Functional expression of the human UDP-galactose transporters in the yeast Saccharomyces cerevisiae. J Biochem 123:912–917PubMedCrossRefGoogle Scholar
  30. Takeshima-Futagami T, Sakaguchi M, Uehara E, Aoki K, Ishida N, Sanai Y, Sugahara Y, Kawakita M (2012) Amino acid residues important for CMP-sialic acid recognition by the CMP-sialic acid transporter: analysis of the substrate specificity of UDP-galactose/CMP-sialic acid transporter chimeras. Glycobiology 22:1731–1740PubMedCrossRefGoogle Scholar
  31. Taki T, Ogura K, Rokukawa C, Hara T, Kawakita M, Endo T, Kobata A, Handa S (1991) Had-1, a uridine 5′-diphosphogalactose transport-defective mutant of mouse mammary tumor cell FM3A: composition of glycolipids, cell growth inhibition by lactosylceramide, and loss of tumorigenicity. Cancer Res 51:1701–1707PubMedGoogle Scholar
  32. Toma L, Pinhal MA, Dietrich CP, Nader HB, Hirschberg CB (1996) Transport of UDP-galactose into the Golgi lumen regulates the biosynthesis of proteoglycans. J Biol Chem 271:3897–3901PubMedCrossRefGoogle Scholar
  33. Yoshioka S, Sun-Wada GH, Ishida N, Kawakita M (1997) Expression of the human UDP-galactose transporter in the Golgi membranes of murine Had-1 cells that lack the endogenous transporter. J Biochem 122:691–695PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Department of Environmental Security SystemChiba Institute of ScienceChoshiJapan

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