Galactose-3-O-Sulfotransferase 1-4 (GAL3ST1-4)

  • Koichi Honke
Reference work entry


Galactose-3-O-sulfotranasferases (Gal3STs) are Golgi-membrane sulfotransferases catalyzing transfer of a sulfate group from the donor substrate, 3′-phosphoadenosine 5′-phosphosulfate (PAPS), to the C3 position of the nonreducing terminal β-Gal residue of carbohydrate chains. So far four Gal3STs have been identified, and their primary structures are found to be similar to each other (El-Fasakhany et al. 2001; Honke et al. 1997, 2001; Seko et al. 2001; Suzuki et al. 2001). The original Gal3ST gene was cloned as a gene that encodes galactosylceramide (GalCer) sulfotransferase (Honke et al. 1997) based on the amino acid sequence of the purified enzyme (Honke et al. 1996). The second through fourth Gal3ST genes were cloned by making use of homology to the GalCer sulfotransferase gene (El-Fasakhany et al. 2001; Honke et al. 2001; Seko et al. 2001; Suzuki et al. 2001). Although the characteristic and biological function of GalCer sulfotransferase (Gal3ST-1) have been extensively studied (Honke et al. 1997; Ishizuka 1997), reports on the other Gal3STs are limited. Therefore, this article mainly focuses on GalCer sulfotransferase (a.k.a. CST, Gal3ST-1).


Human Colon Carcinoma Cell Metachromatic Leukodystrophy Nonreducing Terminus Spinal Cord Magnetic Resonance Imaging Human Renal Cancer Cell 
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  1. Balbaa M, Honke K, Makita A (1996) Regulation of glycolipid sulfotransferase by tyrosine kinases in human renal cancer cells. Biochim Biophys Acta 1299:141–145PubMedCrossRefGoogle Scholar
  2. Capon C, Wieruszeski JM, Lemoine J, Byrd JC, Leffler H, Kim YS (1997) Sulfated Lewis x determinants as a major structural motif in glycans from LS174T-HM7 human colon carcinoma mucin. J Biol Chem 272:31957–31968PubMedCrossRefGoogle Scholar
  3. Cheng X, Zhang Y, Kotani N, Watanabe T, Lee S, Wang X, Kawashima I, Tai T, Taniguchi N, Honke K (2005) Production of a recombinant single-chain variable-fragment (scFv) antibody against sulfoglycolipid. J Biochem 137:415–421PubMedCrossRefGoogle Scholar
  4. Eckhardt M, Fewou SN, Ackermann I, Gieselmann V (2002) N-glycosylation is required for full enzymic activity of the murine galactosylceramide sulphotransferase. Biochem J 368:317–324PubMedCrossRefGoogle Scholar
  5. El-Fasakhany FM, Uchimura K, Kannagi R, Muramatsu T (2001) A novel human Gal-3-O-sulfotransferase: molecular cloning, characterization and its implications in biosynthesis of 3′-sulfo Lewisx. J Biol Chem 276:26988–26994PubMedCrossRefGoogle Scholar
  6. Farooqui AA, Rebel G, Mandel P (1977) Sulphatide metabolism in brain. Life Sci 20:569–583PubMedCrossRefGoogle Scholar
  7. Feizi T, Glaustian C (1999) Novel oligosaccharide ligands and ligand-processing pathways for the selectins. Trends Biochem Sci 24:369–372PubMedCrossRefGoogle Scholar
  8. Guchhait P, Shrimpton CN, Honke K, Rumbaut RE, Lopez JA, Thiagarajan P (2008) Effect of an anti-sulfatide single-chain antibody probe on platelet function. Thromb Haemost 99:552–557PubMedGoogle Scholar
  9. Hess B, Saftig P, Hartmann D, Coenen R, Lüllmann-Rauch R, Goebel HH, Evers M, von Figura K, D’Hooge R, Nagels G, De Deyn P, Peters C, Gieselmann V (1996) Phenotype of arylsulfatase A-deficient mice: relationship to human metachromatic leukodystrophy. Proc Natl Acad Sci U S A 93:14821–14826PubMedCentralPubMedCrossRefGoogle Scholar
  10. Hirahara Y, Tsuda M, Wada Y, Honke K (2000) cDNA cloning, genomic cloning, and tissue-specific regulation of mouse cerebroside sulfotransferase. Eur J Biochem 267:1909–1916PubMedCrossRefGoogle Scholar
  11. Hirahara Y, Bansal R, Honke K, Ikenaka K, Wada Y (2004) Sulfatide is a negative regulator of oligodendrocyte differentiation: development in sulfatide-null mice. Glia 45:269–277PubMedCrossRefGoogle Scholar
  12. Honke K (2013) Biosynthesis and biological function of sulfoglycolipids. Proc Jap Acad Ser B 89:129–138Google Scholar
  13. Honke K, Taniguchi N (2002) Sulfotransferases and sulfated oligosaccharides. Med Res Rev 22:637–654PubMedCrossRefGoogle Scholar
  14. Honke K, Yamane M, Ishii A, Kobayashi T, Makita A (1996) Purification and characterization of 3′-phosphoadenosine-5′-phosphosulfate: GalCer sulfotransferase from human renal cancer cells. J Biochem 119:421–427PubMedCrossRefGoogle Scholar
  15. Honke K, Tsuda M, Hirahara Y, Ishii A, Makita A, Wada Y (1997) Molecular cloning and expression of cDNA encoding human 3′-phosphoadenylylsulfate:galactosylceramide 3′-sulfotransferase. J Biol Chem 272:4864–4868PubMedCrossRefGoogle Scholar
  16. Honke K, Tsuda M, Hirahara Y, Miyao N, Tsukamoto T, Satoh M, Wada Y (1998) Cancer-associated expression of glycolipid sulfotransferase gene in human renal cell carcinoma cells. Cancer Res 58:3800–3805PubMedGoogle Scholar
  17. Honke K, Tsuda M, Koyota S, Wada Y, Tanaka N, Ishizuka I, Nakayama J, Taniguchi N (2001) Molecular cloning and characterization of a human β-Gal 3′-sulfotransferase which acts on both type 1 and type 2 (Galβ1,3/1,4GlcNAc-R) oligosaccharides. J Biol Chem 276:267–274PubMedCrossRefGoogle Scholar
  18. Honke K, Hirahara Y, Dupree J, Suzuki K, Popko B, Fukushima K, Fukushima J, Nagasawa T, Yoshida N, Wada Y, Taniguchi N (2002) Paranodal junction formation and spermatogenesis require sulfoglycolipids. Proc Natl Acad Sci U S A 99:4227–4232PubMedCentralPubMedCrossRefGoogle Scholar
  19. Ikeda N, Eguchi H, Nishihara S, Narimatsu H, Kannagi R, Irimura T, Ohata M, Matsuda H, Taniguchi N, Honke K (2001) A remodeling system of the 3′-sulfo-Lewis a and 3′-sulfo-Lewis x epitopes. J Biol Chem 276:38588–38594PubMedCrossRefGoogle Scholar
  20. Ishibashi T, Dupree JL, Ikenaka K, Hirahara Y, Honke K, Peles E, Popko B, Suzuki K, Nishino H, Baba H (2002) A myelin galactolipid, sulfatide, is essential for maintenance of ion channels on myelinated axon but not essential for initial cluster formation. J Neurosci 22:6507–6514PubMedGoogle Scholar
  21. Ishizuka I (1997) Chemistry and functional distribution of sulfoglycolipids. Prog Lipid Res 36:245–319PubMedCrossRefGoogle Scholar
  22. Izawa M, Kumamoto K, Mitsuoka C, Kanamori A, Ohmori K, Ishida H, Nakamura S, Kurata-Miura K, Sasaki K, Nishi T, Kannagi R (2000) Expression of sialyl 6-sulfo Lewis x is inversely correlated with conventional sialyl Lewis x expression in human colorectal cancer. Cancer Res 60:1410–1416PubMedGoogle Scholar
  23. Kajigaya H, Tanaka KF, Hayashi A, Suzuki A, Ishibashi T, Ikenaka K, Baba H (2011) Increased numbers of oligodendrocyte lineage cells in the optic nerves of cerebroside sulfotransferase knockout mice. Proc Jpn Acad Ser B 87:415–424CrossRefGoogle Scholar
  24. Kakuta Y, Pedersen LG, Pedersem LC, Negishi M (1998) Conserved structural motifs in the sulfotransferase family. Trends Biochem Sci 23:129–130PubMedCrossRefGoogle Scholar
  25. Kawano M, Honke K, Tachi M, Gasa S, Makita A (1989) An assay method for ganglioside synthase using anion-exchange chromatography. Anal Biochem 182:9–15PubMedCrossRefGoogle Scholar
  26. Kobayashi T, Honke K, Kamio K, Sakakibara N, Gasa S, Miyao N, Tsukamoto T, Ishizuka I, Miyazaki T, Makita A (1993a) Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells. Br J Cancer 67:76–80PubMedCentralPubMedCrossRefGoogle Scholar
  27. Kobayashi T, Honke K, Gasa S, Kato N, Miyazaki T, Makita A (1993b) Epidermal growth factor elevates the activity levels of glycolipid sulfotransferases in renal-cell-carcinoma cells. Int J Cancer 55:448–452PubMedCrossRefGoogle Scholar
  28. Kobayashi T, Honke K, Gasa S, Imai S, Tanaka J, Miyazaki T, Makita A (1993c) Regulation of activity levels of glycolipid sulfotransferases by transforming growth factor α in renal cell carcinoma cells. Cancer Res 53:5638–5642PubMedGoogle Scholar
  29. Kobayashi T, Honke K, Gasa S, Sugiura M, Miyazaki T, Ishizuka I, Makita A (1993d) Involvement of protein kinase C in the regulation of glycolipid sulfotransferase activity levels in renal cell carcinoma cells. Cancer Res 53:2484–2489PubMedGoogle Scholar
  30. Kobayashi T, Honke K, Gasa S, Miyazaki T, Tajima H, Matsumoto K, Nakamura T, Makita A (1994) Hepatocyte growth factor elevates the activity levels of glycolipid sulfotransferases in renal cell carcinoma cells. Eur J Biochem 219:407–413PubMedCrossRefGoogle Scholar
  31. Lingwood CA (1985) Developmental regulation of galactoglycerolipid and galactosphingolipid sulphation during mammalian spermatogenesis. Biochem J 231:393–400PubMedGoogle Scholar
  32. Marcus J, Honigbaum S, Shroff S, Honke K, Rosenbluth J, Dupree JL (2006) Sulfatide is essential for the maintenance of CNS myelin and axon structure. Glia 53:372–381PubMedCrossRefGoogle Scholar
  33. Nakajima T, Kamijo Y, Yuzhe H, et al (2012) Peroxisome proliferator-activated receptor a mediates enhancement of gene expression of cerebroside sulfotransferase in several murine organs. Glycoconj J 30:553–560Google Scholar
  34. Ogawa D, Shikata K, Honke K, Sato S, Mattsuda M, Nagase R, Tone A, Okada S, Usui H, Wada J, Miyasaka M, Kawashima H, Suzuki Y, Suzuki T, Taniguchi N, Hirahara Y, Tadano-Aritomi K, Ishizuka I, Tedder TF, Makino H (2004) Cerebroside sulfotransferase deficiency ameliorates l-selectin-dependent monocyte infiltration in the kidney after ureteral obstruction. J Biol Chem 279:2085–2090PubMedCrossRefGoogle Scholar
  35. Ramakrishnan H, Khalaj Hedayati K, Lüllmann-Rauch R, Wessig C, Fewou SN, Maier H, Goebel HH, Gieselmann V, Eckhardt M (2007) Increasing sulfatide synthesis in myelin-forming cells of arylsulfatase A-deficient mice causes demyelination and neurological symptoms reminiscent of human metachromatic leukodystrophy. J Neurosci 27:9482–9490PubMedCrossRefGoogle Scholar
  36. Sakakibara N, Gasa S, Kamio K, Makita A, Koyanagi T (1989) Association of elevated sulfatides and sulfotranasferase activities with human renal cell carcinoma. Cancer Res 49:335–339PubMedGoogle Scholar
  37. Seko A, Hara-Kuge S, Yamashita K (2001) Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galβ1-3GalNAc residue in O-glycans. J Biol Chem 276:25697–25704PubMedCrossRefGoogle Scholar
  38. Seko A, Nagata K, Yonezawa S, Yamashita K (2002) Down-regulation of Gal 3-O-sulfotransferase-2 (Gasl3ST-2) expression in human colonic non-mucinous adenocarcinoma. Jpn J Cancer Res 93:507–515PubMedCrossRefGoogle Scholar
  39. Shroff SM, Pomicter AD, Chow WN, Fox MA, Colello RJ, Henderson SC, Dupree JL (2009) Adult CST-null mice maintain an increased number of oligodendrocytes. J Neurosci Res 87:3403–3414PubMedCrossRefGoogle Scholar
  40. Suzuki A, Hiraoka N, Suzuki M, Angata K, Misra AK, McAuliffe J, Hindsgaul O, Fukuda M (2001) Molecular cloning and expression of a novel human β-Gal-3-O-sulfotransferase that acts preferentially on N-acetyllactosamine in N- and O-glycans. J Biol Chem 276:24388–24395PubMedCrossRefGoogle Scholar
  41. Suzuki T, Kosaka-Suzuki N, Pack S, Shin D, Yoon J, Abdullaev Z, Pugacheva E, Morse HC III, Loukinov D, Lobanenkov V (2010) Expression of a testis-specific form of Gal3st1 (CST), a gene essential for spermatogenesis, is regulated by the CTCF paralogous gene BORIS. Mol Cell Biol 30:2473–2484PubMedCentralPubMedCrossRefGoogle Scholar
  42. Takano M, Hikishima K, Fujiyoshi K, Shibata S, Yasuda A, Konomi T, Hayashi A, Baba H, Honke K, Toyama Y, Okano H, Nakamura M (2012) MRI characterization of paranodal junction failure and related spinal cord changes in mice. PLoS One 7:e52904PubMedCentralPubMedCrossRefGoogle Scholar
  43. Tennekoon G, Aitchison S, Zaruba M (1985) Purification and characterization of galactocerebroside sulfotransferase from rat kidney. Arch Biochem Biophys 240:932–944PubMedCrossRefGoogle Scholar
  44. Tsuda M, Egashira M, Niikawa N, Wada Y, Honke K (2000) Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene. Eur J Biochem 267:2672–2679PubMedCrossRefGoogle Scholar
  45. Vavasseur F, Dole K, Yang J, Matta KL, Myerscough N, Cornfield A, Paraskeva C, Brockhausen I (1994) O-glycan biosynthesis in human colorectal adenoma cells during progression to cancer. Eur J Biochem 222:415–424PubMedCrossRefGoogle Scholar
  46. Vos JP, Lopes-Cardozo M, Gadella BM (1994) Metabolic and functional aspects of sulfogalactolipids. Biochim Biophys Acta 1211:125–149PubMedCrossRefGoogle Scholar
  47. Yabunaka N, Honke K, Ishii A, Ogiso Y, Kuzumaki N, Agishi Y, Makita A (1997) Involvement of Ras in the expression of glycolipid sulfotransferase in human renal cancer cells. Int J Cancer 71:620–623PubMedCrossRefGoogle Scholar
  48. Yaghootfam A, Sorkalla T, Häberlein H, Gieselmann V, Kappler J, Eckhardt M (2007) Cerebroside sulfotransferase forms homodimers in living cells. Biochemistry 46:9260–9269PubMedCrossRefGoogle Scholar
  49. Yamachika T, Nakanishi H, Inada K, Kitoh K, Kato T, Irimura T, Tatematsu M (1997) Reciprocal control of colon-specific sulfomucin and sialosyl-Tn antigen expression in human colorectal neoplasia. Virchows Arch 431:25–30PubMedCrossRefGoogle Scholar
  50. Zhang Y, Hayashi Y, Cheng X, Watanabe T, Wang X, Taniguchi N, Honke K (2005) Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis. Glycobiology 15:649–654PubMedCrossRefGoogle Scholar
  51. Zhou Z, Thiagarajan P, Udden M, Lòpez JA, Guchhait P (2011) Erythrocyte membrane sulfatide plays a crucial role in the adhesion of sickle erythrocytes to endothelium. Thromb Haemost 105:1046–1052PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Department of BiochemistryKochi University Medical SchoolNankokuJapan

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