Fucokinase (FUK)

Reference work entry


Fucose from extracellular sources or the degradation of glycoconjugates is converted after cellular uptake by a salvage pathway leading to the formation of GDP-fucose. The first step requires the synthesis of β-l-fucose-1-phosphate by an ATP-dependent kinase reaction, which is catalyzed by l-fucose kinase (fucokinase) (Fig. 143.1). GDP-fucose is then formed by action of GDP-fucose pyrophosphorylase. Depending on tissue and physiological state, the salvage pathway usually contributes about 10 % to the total GDP-fucose pool, which is mainly fed by de novo synthesis from GDP-mannose (Fig. 143.1). Afterwards GDP-fucose is transported to the Golgi apparatus, where the fucose moiety is transferred to growing glycoconjugates by specific fucosyltransferases.


Salvage Pathway Bifunctional Enzyme Radiometric Assay Porcine Thyroid Extracellular Source 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Butler W, Serif GS (1985) Fucokinase, its anomeric specificity and mechanism of phosphate group transfer. Biochim Biophys Acta 829:238–243PubMedCrossRefGoogle Scholar
  2. Coffey JW, Miller ON, Sellinger OZ (1964) The metabolism of l-fucose in the rat. J Biol Chem 239:4011–4017PubMedGoogle Scholar
  3. Hackenberger CP, Hinderlich S (2007) Chemoselective labeling of engineered fucosylated glycoproteins. Chembiochem 15:1763–1765CrossRefGoogle Scholar
  4. Hinderlich S, Berger M, Blume A, Chen H, Ghaderi D, Bauer C (2002) Identification of human l-fucose kinase amino acid sequence. Biochem Biophys Res Commun 294:650–654PubMedCrossRefGoogle Scholar
  5. Hocher B, Abou-Rebyeh F, Bauer C (1993) Influence of dopaminergic agonists/antagonists on fucose metabolism in the rat brain. Eur J Clin Chem Clin Biochem 31:347–351PubMedGoogle Scholar
  6. Ishihara H, Massaro DJ, Heath EC (1968) The metabolism of l-fucose. 3. The enzymatic synthesis of beta-l-fucose 1-phosphate. J Biol Chem 243:1103–1109PubMedGoogle Scholar
  7. Jork R, Schmitt M, Lössner B, Matthies H (1984) Dopamine stimulated l-fucose incorporation into brain proteins is related to an increase in fucokinase activity. Biomed Biochim Acta 43:261–270PubMedGoogle Scholar
  8. Kilker RD, Shuey DK, Serif GS (1979) Isolation and properties of porcine thyroid fucokinase. Biochim Biophys Acta 570:271–283PubMedCrossRefGoogle Scholar
  9. Kotake T, Hojo S, Tajima N, Matsuoka K, Koyama T, Tsumuraya (2008) A bifunctional enzyme with l-fucokinase and GDP-l-fucose pyrophosphorylase activities salvages free l-fucose in Arabidopsis. J Biol Chem 283:8125–8135PubMedCrossRefGoogle Scholar
  10. Lee WH, Shin SY, Kim MD, Han NS, Seo JH (2012) Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-l-fucose production in recombinant Escherichia coli. Appl Microbiol Biotechnol 93:2327–2334PubMedCrossRefGoogle Scholar
  11. Liu TW, Ito H, Chiba Y, Kubota T, Sato T, Narimatsu H (2011) Functional expression of l-fucokinase/guanosine 5′-diphosphate-l-fucose pyrophosphorylase from bacteroides fragilis in saccharomyces cerevisiae for the production of nucleotide sugars from exogenous monosaccharides. Glycobiology 21:1228–1236PubMedCrossRefGoogle Scholar
  12. Liu TW, Kaji H, Togayachi A, Ito H, Sato T, Narimatsu H (2012) A chemoenzymatic approach toward the identification of fucosylated glycoproteins and mapping of N-glycan sites. Glycobiology 22:630–637PubMedCrossRefGoogle Scholar
  13. Lössner B, Rose SP (1983) Passive avoidance training increases fucokinase activity in right forebrain base of day-old chicks. J Neurochem 41:1357–1363PubMedCrossRefGoogle Scholar
  14. Miller EN, Rupp AL, Lindberg MK, Wiese TJ (2005) Tissue distribution of l-fucokinase in rodents. Comp Biochem Physiol B Biochem Mol Biol 140:513–520PubMedCrossRefGoogle Scholar
  15. Niittymäki J, Mattila P, Roos C, Huopaniemi L, Sjöblom S, Renkonen R (2004) Cloning and expression of murine enzymes involved in the salvage pathway of GDP-l-fucose. Eur J Biochem 271:78–86PubMedCrossRefGoogle Scholar
  16. Park SH, Pastuszak I, Drake R, Elbein AD (1998) Purification to apparent homogeneity and properties of pig kidney l-fucose kinase. J Biol Chem 273:5685–5691PubMedCrossRefGoogle Scholar
  17. Popov N, Schmidt S, Schulzeck S, Jork R, Lössner B, Matthies H (1983) Changes in activities of fucokinase and fucosyltransferase in rat hippocampus after acquisition of a brightness discrimination reaction. Pharmacol Biochem Behav 19:43–47PubMedCrossRefGoogle Scholar
  18. Reutter W, Bauer C (1985) Inhibitors of glycoprotein biosynthesis. Adv Enzyme Regul 24:405–416PubMedCrossRefGoogle Scholar
  19. Richards WL, Serif GS (1977) Canine thyroid fucokinase. Biochim Biophys Acta 484:353–367PubMedCrossRefGoogle Scholar
  20. Richards WL, Serif GS (1979) Effects of hypophysectomy and TSH on thyroidal fucokinase in rats. Proc Soc Exp Biol Med 162:291–294PubMedCrossRefGoogle Scholar
  21. Richards WL, Kilker RD, Serif GS (1978) Metabolite control of l-fucose utilization. J Biol Chem 253:8359–8361PubMedGoogle Scholar
  22. Wang W, Hu T, Frantom PA, Zheng T, Gerwe B, Del Amo DS, Garret S, Seidel RD 3rd, Wu P (2009) Chemoenzymatic synthesis of GDP-l-fucose and the Lewis X glycan derivatives. Proc Natl Acad Sci U S A 106:16096–16101PubMedCentralPubMedCrossRefGoogle Scholar
  23. Zeitler R, Danneschewski S, Lindhorst T, Thiem J, Reutter W (1997) Inhibition of l-fucokinase from rat liver by l-fucose analogues in vitro. J Enzyme Inhib 11:265–273PubMedCrossRefGoogle Scholar
  24. Zhao G, Guan W, Cai L, Wang PG (2010) Enzymatic route to preparative-scale synthesis of UDP-GlcNAc/GalNAc, their analogues and GDP-fucose. Nat Protoc 5:636–646PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Department of Life Sciences and TechnologyBeuth Hochschule für Technik Berlin – University of Applied SciencesBerlinGermany

Personalised recommendations