Handbook of Glycosyltransferases and Related Genes pp 1649-1655 | Cite as
Dolichyl-Phosphate Beta-Glucosyltransferase (ALG5)
Dolichyl-phosphate beta-glucosyltransferase (ALG5), which is localized on the rough endoplasmic reticulum (rER) membrane, catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to dolichyl-phosphate (Dol-P) to synthesize dolichol-phosphate glucose (Dol-P-Glc, DPG, Glc-P-Dol or GPD). Therefore, this enzyme is also called Dol-P-Glc synthase. Dol-P-Glc produced by this enzyme is utilized for glucosylation of intermediate dolichol-linked oligosaccharide (DLO), N-glycan precursor, by three glucosyltransferases (ALG6, ALG8, and ALG10 proteins) at final stages of its assembly pathway.
KeywordsNonpermissive Temperature ALG5 Gene Alg5 Mutation Double Mutant Cell Yeast Microsome
- Burda P, Borsig L, de Rijk-van Andel J, Wevers R, Jaeken J, Carchon H, Berger EG, Aebi M (1998) A novel carbohydrate-deficient glycoprotein syndrome characterized by a deficiency in glucosylation of the dolichol-linked oligosaccharide. J Clin Invest 102:647–652PubMedCentralPubMedCrossRefGoogle Scholar
- Drake RR, Igari Y, Lester R, Elbein AD, Radominska A (1992) Application of 5-azido-UDP-glucose and 5-azido-UDP-glucuronic acid photoaffinity probes for the determination of the active site orientation of microsomal UDP-glucosyltransferases and UDP-glucuronosyltransferases. J Biol Chem 267:11360–11365PubMedGoogle Scholar