Abstract
Many proteins synthesized through the secretory pathway are posttranslationally modified by N-glycosylation. Alpha-mannosidase II (MAN2A1) and alpha-mannosidase IIx (MAN2A2) are key enzymes that convert precursor high mannose-type N-glycans to mature complex-type structures. While alpha-mannosidase II activity had been characterized by the time the mechanism of N-glycan processing was recognized (Kornfeld and Kornfeld 1985), investigators did not discover an alternative processing pathway until Man2a1 null mutant mice were generated and found capable of synthesizing complex-type N-glycans (Chui et al. 1997). Man2a2 then became a candidate for mediating that alternate pathway of N-glycan synthesis, but support for the pathway was lacking until creation of Man2a1/Man2a2 double knockout mice, which showed a complete loss in the ability to synthesize complex-type N-glycans (Fig. 117.1) (Akama et al. 2006).
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References
Akama TO, Fukuda MN (2006) N-Glycan structure analysis using lectins and an alpha-mannosidase activity assay. Methods Enzymol 416:304–314
Akama TO, Nakagawa H, Sugihara K, Narisawa S, Ohyama C, Nishimura S, O’Brien DA, Moremen KW, Millan JL, Fukuda MN (2002) Germ cell survival through carbohydrate-mediated interaction with sertoli cells. Science 295:124–127
Akama TO, Nakagawa H, Wong NK, Sutton-Smith M, Dell A, Morris HR, Nakayama J, Nishimura S, Pai A, Moremen KW et al (2006) Essential and mutually compensatory roles of alpha-mannosidase II and alpha-mannosidase IIx in N-glycan processing in vivo in mice. Proc Natl Acad Sci USA 103:8983–8988
Bulun SE, Zeitoun KM, Takayama K, Sasano H (2000) Estrogen biosynthesis in endometriosis: molecular basis and clinical relevance. J Mol Endocrinol 25:35–42
Chui D, Oh-Eda M, Liao YF, Panneerselvam K, Lal A, Marek KW, Freeze HH, Moremen KW, Fukuda MN, Marth JD (1997) Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis. Cell 90:157–167
Dall’Olio GM, Laayouni H, Luisi P, Sikora M, Montanucci L, Bertranpetit J (2012) Distribution of events of positive selection and population differentiation in a metabolic pathway: the case of asparagine N-glycosylation. BMC Evol Biol 12:98
Flores I, Rivera E, Mousses S, Chen Y, Rozenblum E (2006) Identification of molecular markers for endometriosis in blood lymphocytes by using deoxyribonucleic acid microarrays. Fertil Steril 85:1676–1683
Kornfeld R, Kornfeld S (1985) Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem 54:631–664
Kroes RA, Dawson G, Moskal JR (2007) Focused microarray analysis of glyco-gene expression in human glioblastomas. J Neurochem 103(Suppl 1):14–24
Misago M, Liao Y-F, Kudo S, Eto S, Mattei M-G, Moremen KW, Fukuda MN (1995) Molecular cloning and expression of cDNAs encoding human a-mannosidase II and a novel a-mannosidase IIx isozyme. Proc Natl Acad Sci USA 92:11766–11770
Ogawa R, Misago M, Fukuda MN, Kudo S, Tsukada J, Morimoto I, Eto S (1996) Structure and transcriptional regulation of human alpha-mannosidase IIX (alpha-mannosidase II isotype) gene. Eur J Biochem 242:446–453
Wei YL, Li CX, Li SB, Liu Y, Hu L (2011) Association study of monoamine oxidase A/B genes and schizophrenia in Han Chinese. Behav Brain Funct: BBF 7:42
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Fukuda, M.N., Sugihara, K., Akama, T.O. (2014). Mannosidase, Alpha, Class 2a2 (MAN2A2). In: Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (eds) Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54240-7_30
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DOI: https://doi.org/10.1007/978-4-431-54240-7_30
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