Abstract
GDP-mannose (GDP-Man) is the central activated sugar nucleotide for mannosylation and is essential for the biosynthesis of N-linked glycoproteins and glycosylphosphatidylinositol anchors (GPI-anchors) in eukaryotes as well as for the formation of bacterial capsular lipopolysaccharides. In addition, GDP-Man is well known to be one of the sources of GDP-fucose (GDP-Fuc), another sugar nucleotide critical for glycosylation in eukaryotes and microbial cells, and is one of the essential intermediates for the biosynthesis of vitamin C (l-ascorbic acid) in plants (Conklin et al. 1999b, 2006). GDP-mannose pyrophosphorylase (GMPP) enzyme, which is highly conserved from bacteria to human, catalyzes the reaction to form GDP-Man from mannose-1-phosphate (Man-1-P) and GDP in the cytosol. Following successful cloning of the gene encoding GMPP from baker’s yeast, Saccharomyces cerevisiae (Hashimoto et al. 1997), homologues of the gene have been cloned from a number of organisms and are categorized into two groups based on complex formation. In one group, which includes many animal, plant, fly, worm, and fission yeast homologues, GMPP forms a heterodimeric complex consisting of an α-subunit, GDP-Man pyrophosphorylase A (GMPPA), and a catalytic β-subunit, GDP-Man pyrophosphorylase B (GMPPB). In the other group, which includes protozoa, budding yeast, and bacteria, GMPP does not have the α-subunit. In yeast such as S. cerevisiae and Candida albicans, the deletion of structural gene coding GMPP is lethal because the lack of GDP-Man leads to the malformation of N-glycans, O-glycans, and GPI-anchors and, therefore, defects in the architecture and integrity of the cell wall (Warit et al. 2000). In contrast, the deletion of gene coding GMPP in the pathogenic protozoan, Leishmania mexicana, does not show lethal phenotype, but unable to infect macrophages or mice due to a defect in the formation of large amounts of mannose-containing glycoconjugates on the cell surface (Garami and Ilg 2001).
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References
Adler LN, Gomez TA, Clarke SG, Linster CL (2011) A novel GDP-d-glucose phosphorylase involved in quality control of the nucleoside diphosphate sugar pool in Caenorhabditis elegans and mammals. J Biol Chem 286:21511–21523
Barth C, Gouzd ZA, Steele HP, Imperio RM (2010) A mutation in GDP-mannose pyrophosphorylase causes conditional hypersensitivity to ammonium, resulting in Arabidopsis root growth inhibition, altered ammonium metabolism, and hormone homeostasis. J Exp Bot 61:379–394
Benton BK, Plump SD, Roos J, Lennarz WJ, Cross FR (1996) Over-expression of S. cerevisiae G1 cyclins restores the viability of alg1 N-glycosylation mutants. Curr Genet 29:106–113
Carlson DM, Hansen RG (1962) The isolation and synthesis of guanosine diphosphate glucose. J Biol Chem 237:1260–1265
Carss KJ, Stevens E, Foley AR, Cirak S, Riemersma M, Torelli S, Hoischen A, Willer T, van Scherpenzeel M, Moore SA, Messina S, Bertini E, Bönnemann CG, Abdenur JE, Grosmann CM, Kesari A, Punetha J, Quinlivan R, Waddell LB, Young HK, Wraige E, Yau S, Brodd L, Feng L, Sewry C, MacArthur DG, North KN, Hoffman E, Stemple DL, Hurles ME, van Bokhoven H, Campbell KP, Lefeber DJ; UK10K Consortium, Lin YY, Muntoni F (2013) Mutations in GDP-mannose pyrophosphorylase B cause congenital and limb-girdle muscular dystrophies associated with hypoglycosylation of α-dystroglycan. Am J Hum Genet 93(1):29–41
Conklin PL, Pallanca JE, Last RL, Smirnoff N (1997) l-ascorbic acid metabolism in the ascorbate-deficient Arabidopsis mutant vtc1. Plant Physiol 115:1277–1285
Conklin PL, Norris SR, Wheeler GL, Williams EH, Smirnoff N, Last RL (1999) Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. Proc Natl Acad Sci USA 96:4198–4203
Conklin PL, Gatzek S, Wheeler GL, Dowdle J, Raymond MJ, Rolinski S, Isupov M, Littlechild JA, Smirnoff N (2006) Arabidopsis thaliana VTC4 encodes l-galactose-1-P phosphatase, a plant ascorbic acid biosynthetic enzyme. J Biol Chem 281:15662–15670
Davis AJ, Perugini MA, Smith BJ, Stewart JD, Ilg T, Hodder AN, Handman E (2004) Properties of GDP-mannose pyrophosphorylase, a critical enzyme and drug target in Leishmania mexicana. J Biol Chem 279:12462–12468
Denton H, Fyffe S, Smith TK (2010) GDP-mannose pyrophosphorylase is essential in the bloodstream form of Trypanosoma brucei. Biochem J 425:603–614
Descoteaux A, Luo Y, Turco SJ, Beverley SM (1995) A specialized pathway affecting virulence glycoconjugates of Leishmania. Science 269:1869–1872
Ferguson MA (1999) The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research. J Cell Sci 112(Pt 17):2799–2809
Garami A, Ilg T (2001) Disruption of mannose activation in Leishmania mexicana: GDP-mannose pyrophosphorylase is required for virulence, but not for viability. EMBO J 20:3657–3666
Hashimoto H, Sakakibara A, Yamasaki M, Yoda K (1997) Saccharomyces cerevisiae VIG9 encodes GDP-mannose pyrophosphorylase, which is essential for protein glycosylation. J Biol Chem 272:16308–16314
Jiang H, Ouyang H, Zhou H, Jin C (2008) GDP-mannose pyrophosphorylase is essential for cell wall integrity, morphogenesis and viability of Aspergillus fumigatus. Microbiology 154:2730–2739
Lackovic K, Parisot JP, Sleebs N, Baell JB, Debien L, Watson KG, Curtis JM, Handman E, Street IP, Kedzierski L (2010) Inhibitors of Leishmania GDP-mannose pyrophosphorylase identified by high-throughput screening of small-molecule chemical library. Antimicrob Agents Chemother 54:1712–1719
Munch-Petersen A, Sillén LG, Ormerod JG, Stenhagen E, Thorell B (1956) Reversible enzymic synthesis of guanosine diphosphate mannose from guanosine triphosphate and mannose-1-phosphate. Acta Chem Scand 10:928–934
Ning B, Elbein AD (1999) Purification and properties of mycobacterial GDP-mannose pyrophosphorylase. Arch Biochem Biophys 362:339–345
Ning B, Elbein AD (2000) Cloning, expression and characterization of the pig liver GDP-mannose pyrophosphorylase. Evidence that GDP-mannose and GDP-Glc pyrophosphorylases are different proteins. FEBS 267:6866–6874
Pelissier M-C, Lesley SA, Kuhn P, Bourne Y (2010) Structural insights into the catalytic mechanism of bacterial guanosine-diphospho-d-mannose pyrophosphorylase and its regulation by divalent ions. J Biol Chem 285:27468–27476
Preiss J, Wood E (1964) Sugar nucleotide reactions in arthrobacter. I. Guanosine diphosphate mannose pyrophosphorylase: purification and properties. J Biol Chem 239:3119–3126
Shinabarger D, Berry A, May TB, Rothmel R, Fialho A, Chakrabarty AM (1991) Purification and characterization of phosphomannose isomerase-guanosine diphospho-d-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa. J Biol Chem 266:2080–2088
Szumilo T, Drake RR, York JL, Elbein AD (1993) GDP-mannose pyrophosphorylase. Purification to homogeneity, properties, and utilization to prepare photoaffinity analogs. J Biol Chem 268:17943–17950
Verachtert H, Rodriguez P, Bass ST, Hansen RG (1966) Purification and properties of guanosine diphosphate hexose pyrophosphorylase from mammalian tissues. J Biol Chem 241:2007–2013
Warit S, Zhang N, Short A, Walmsley RM, Oliver SG, Stateva LI (2000) Glycosylation deficiency phenotypes resulting from depletion of GDP-mannose pyrophosphorylase in two yeast species. Mol Microbiol 36:1156–1166
Wu B, Zhang Y, Zheng R, Guo C, Wang PG (2002) Bifunctional phosphomannose isomerase/GDP-d-mannose pyrophosphorylase is the point of control for GDP-d-mannose biosynthesis in Helicobacter pylori. FEBS Lett 519:87–92
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Hirayama, H., Suzuki, T. (2014). GDP-Mannose Pyrophosphorylase A,B (GMPPA,B). In: Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (eds) Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54240-7_155
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DOI: https://doi.org/10.1007/978-4-431-54240-7_155
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