Handbook of Glycosyltransferases and Related Genes pp 1425-1437 | Cite as
UDP-Glucose 6-Dehydrogenase (UGDH)
Uridine diphosphate-α-d-glucose 6-dehydrogenase (UGDH, EC 22.214.171.124) is the four-electron transfer enzyme responsible for the oxidation of 6-hydroxyl group of UDP-α-d-glucose (UDP-Glc) to produce UDP-α-d-glucuronic acid (UDP-GlcA). The latter is the substrate for numerous transferases (described elsewhere in this book) which are engaged in the synthesis of hyaluronan (HA), heparin/heparan sulfate, and chondroitin/dermatan sulfate and numerous glucuronides essential in the detoxification of xenobiotics and toxic or insoluble products of metabolism. UDP-GlcA can also be enzymatically decarboxylated to form UDP-xylose, the priming sugar in the synthesis of sulfated glycosaminoglycans (GAGs). UDP-xylose is also an endogenous negative feedback inhibitor of UGDH activity.
KeywordsSulfated Glycosaminoglycan Cysteine Thiol Hyaluronan Synthesis Bovine Enzyme Crude Tissue Extract
- Bontemps Y, Vuillermoz B, Antonicelli F, Perreau C, Danan JL, Maquart FX, Wegrowski Y (2003) Specific protein-1 is a universal regulator of UDP-glucose dehydrogenase expression: its positive involvement in transforming growth factor-beta signaling and inhibition in hypoxia. J Biol Chem 278:21566–21575PubMedCrossRefGoogle Scholar
- Hyde AS, Farmer EL, Easley KE, van Lammeren K, Christoffels VM, Barycki JJ, Bakkers J, Simpson MA (2012) UDP-glucose dehydrogenase polymorphisms from patients with congenital heart valve defects disrupt enzyme stability and quaternary assembly. J Biol Chem 287:32708–32716PubMedCrossRefGoogle Scholar
- Vigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F, Nardi I, Hascall VC, De Luca G, Passi A (2006) Molecular cloning and characterization of UDP-glucose dehydrogenase from the amphibian Xenopus laevis and its involvement in hyaluronan synthesis. J Biol Chem 281:8254–8263PubMedCrossRefGoogle Scholar