ST6 N-Acetylgalactosaminide Alpha-2,6-Sialyltransferase 2 (ST6GALNAC2)

  • Shuichi Tsuji
  • Shou Takashima
Reference work entry


ST6GalNAc-II is a CMP-sialic acid – N-acetylgalactosaminide α2-6-sialyltransferase (GalNAcα2-6-sialyltransferase) – i.e., transfers sialic acid from CMP-sialic acid (CMP-Sia) to the N-acetylgalactosamine residues of glycoconjugates through an α2,6-linkage. Like other sialyltransferases, ST6GalNAc-II exhibits type II membrane protein topology and is classified into CAZy glycosyltransferase family 29 (Coutinho et al. 2003). From amino acid sequence similarities, substrate specificities, and gene structures, ST6GalNAc-I and ST6GalNAc-II can be classified into one subfamily. ST6GalNAc-I exhibits a broader substrate specificity for GalNAc-O-Ser/Thr, Galβl-3GalNAc-O-Ser/Thr, and NeuAcα2-3Galβl-3GalNAc-O -Ser/Thr (Kurosawa et al. 1994, 2000; Ikehara et al. 1999; Kono et al. 2000; Samyn-Petit et al. 2000; Marcos et al. 2004). On the other hand, ST6GalNAc-II exhibits a narrower substrate specificity using Galβl-3GalNAcαl-O-Ser/Thr and Neu5Acα2-3Galβl-3GalNAcαl-O-Ser/Thr as substrates (Kurosawa et al. 1994, 1996).


Mammary Gland Sialic Acid MKN45 Cell Acceptor Substrate Fusion Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Coutinho PM, Deleury E, Davies GJ, Henrissat B (2003) An evolving hierarchical family classification for glycosyltransferases. J Mol Biol 328:307–317PubMedCrossRefGoogle Scholar
  2. Gottschalk A, Bhargava AS (1972) In: Gottschalk A (ed) Glycoproteins: their composition, structure and function. Elsevier, New YorkGoogle Scholar
  3. Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R (2005) The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. Glycobiology 15:805–817PubMedCrossRefGoogle Scholar
  4. Harduin-Lepers A (2010) Comprehensive analysis of sialyltransferases in vertebrate genomes. Glycobiol Insights 2:29–61CrossRefGoogle Scholar
  5. Ikehara Y, Naoya Kojima N, Kurosawa N, Kudo T, Nishihara S, Morozumi K, Kono M, Tsuji S, Narimatsu H (1999) Cloning and expression of a human gene encoding an N- acetylgalactosamine-a2,6-sialyltransferase(ST6GalNAc-I) which is a candidate for synthesis of cancer-associated sialyl-Tn. Glycobiology 9:1213–1224PubMedCrossRefGoogle Scholar
  6. Kono M, Yoshida Y, Kojima N, Tsuji S (1996) Molecular cloning and expression of a fifth type of a2,8-sialyltransferase (ST8SiaV): its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3. J Biol Chem 271:29366–29371PubMedCrossRefGoogle Scholar
  7. Kono M, Tsuda T, Ogata S, Takashima S, Liu H, Hamamoto T, Itzkowitz SH, Nishimura S, Tsuji S (2000) Re-defined substrate specificity of ST6GalNAc-II: a second candidate sialyl-Tn synthase. Biochem Biophys Res Commun 272:94–97PubMedCrossRefGoogle Scholar
  8. Kurosawa N, Kojima N, Inoue M, Hamamoto T, Tsuji S (1994) Cloning and expression of Galβl,3GalNAc-specific GalNAc α2,6-sialyltransferase. J Biol Chem 269:19048–19053PubMedGoogle Scholar
  9. Kurosawa N, Inoue M, Yoshida Y, Tsuji S (1996) Molecular cloning and genomic analysis of mouse Galβl,3GalNAc-specific GalNAc α2,6-sialyltransferase. J Biol Chem 271:15109–15116PubMedCrossRefGoogle Scholar
  10. Kurosawa N, Takashima S, Kono M, Ikehara Y, Inoue M, Tachida Y, Narimatsu H, Tsuji S (2000) Molecular cloning and genomic analysis of mouse GalNAc-α2,6-sialyltransferase(ST6GalNAc-I). J Biochem 127:845–854PubMedCrossRefGoogle Scholar
  11. Lee YC, Kaufmann M, Kitazume-Kawaguchi S, Kono M, Takashima S, Kurosawa N, Liu H, Pircher H, Tsuji S (1999) Molecular cloning and functional expression of two members of mouse NeuAcα2,3Galβ1,3GalNAc GalNAcα2,6-sialyltransferase family, ST6GalNAc III and IV. J Biol Chem 274:11958–11967. doi:10.1074/jbc.274.17.11958PubMedCrossRefGoogle Scholar
  12. Marcos NT, Pinho S, Grandela C, Cruz A, Samyn-Petit B, Harduin-Lepers A, Almeida R, Silva F, Morais V, Costa J, Kihiberg J, Clausen H, Reis CA (2004) Role of human ST6GalNAc-I and ST6GalNAc-II in the synthesis of the cancer-associated Sialyl-Tn antigen. Cancer Res 64:7050–7057PubMedCrossRefGoogle Scholar
  13. Samyn-Petit B, Krzewinski-Recchi MA, Steelant WF, Delannoy P, Harduin-Lepers A (2000) Molecular cloning and functional expression of human ST6GalNAc II. Molecular expression in various human cultured cells. Biochim Biophys Acta 1474:201–211PubMedCrossRefGoogle Scholar
  14. Takashima S (2008) Characterization of mouse sialyltransferase genes: their evolution and diversity. Biosci Biotechnol Biochem 72:1155–1167PubMedCrossRefGoogle Scholar
  15. Takashima S, Tsuji S (2011) Functional diversity of mammalian sialyltransferases. Trends in Glycosci Glycotech 23:178–193CrossRefGoogle Scholar
  16. Tsuji S (1999) Molecular cloning and characterization of sialyltransferases. In: Inoue Y, Lee YC, Troy FA (eds) Sialobiology and other novel forms of glycosylation. Gakushin, Osaka, pp 145–154Google Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Institute of Glycoscience, Tokai UniversityHiratsukaJapan
  2. 2.Laboratory of GlycobiologyThe Noguchi InstituteItabashiJapan

Personalised recommendations