ST6 N-Acetylgalactosaminide Alpha-2,6-Sialyltransferase 1 (ST6GALNAC1)

  • Shuichi Tsuji
  • Shou Takashima
Reference work entry


ST6GalNAc-I is a CMP-sialic acid, N-acetylgalactosaminide α2-6-sialyltransferase (GalNAcα2-6-sialyltransferase), that transfers sialic acid from CMP-sialic acid (CMP-Sia) to the N-acetylgalactosamine residues of glycoconjugates through an α2,6-linkage. Like other sialyltransferases, ST6GalNAc-I exhibits type II membrane protein topology and is classified into CAZy glycosyltransferase family 29 (Coutinho et al. 2003).


Sialic Acid MKN45 Cell Acceptor Substrate Fusion Enzyme Human Colorectal Cancer Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Baldus SE, Hanisch FG (2000) Biochemistry and pathological importance of mucin-associated antigens in gastrointestinal neoplasia. Adv Cancer Res 79:201–248PubMedCrossRefGoogle Scholar
  2. Coutinho PM, Deleury E, Davies GJ, Henrissat B (2003) An evolving hierarchical family classification for glycosyltransferases. J Mol Biol 328:307–317PubMedCrossRefGoogle Scholar
  3. Harduin-Lepers A (2010) Comprehensive analysis of sialyltransferases in vertebrate genomes. Glycobiol Insights 2:29–61CrossRefGoogle Scholar
  4. Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R (2005) The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach. Glycobiology 15:805–817. doi:10.1093/glycob/cwi063PubMedCrossRefGoogle Scholar
  5. Holmberg LA, Oparin DV, Gooley T, Sandmaier BM (2003) The role of cancer vaccines following autologous stem cell rescue in breast and ovarian cancer patients: experience with the STn-KLH vaccine (Theratope(R)). Clin Breast Cancer 3:S144–S151PubMedCrossRefGoogle Scholar
  6. Ikehara Y, Naoya Kojima N, Kurosawa N, Kudo T, Nishihara S, Morozumi K, Kono M, Tsuji S, Narimatsu H (1999) Cloning and expression of a human gene encoding an N-acetylgalactosamine-α2,6-sialyltransferase (ST6GalNAc-I) which is a candidate for synthesis of cancer-associated Sialyl-Tn. Glycobiology 9:1213–1224PubMedCrossRefGoogle Scholar
  7. Itzkowitz SH, Bloom EJ, Kokal WA, Modin G, Hakomori S, Kirn YS (1990) Sialosyl-Tn. A novel mucin antigen associated with prognosis in colorectal cancer patients. Cancer (Phila) 166:1960–1966CrossRefGoogle Scholar
  8. Kirn GE, Bae HI, Park HU, Kuan S-H, Crawley SC, Ho JL, Ys K (2002) Aberrant expression ofMUC5AC and MUC6 gastric mucins and sialyl Tn antigen in intraepithelial neoplasms of the pancreas. Gastroenterology 123:1052–1060CrossRefGoogle Scholar
  9. Kobayashi H, Terao T, Kawashima Y (1992) Serum sialyl Tn as an independent predictor of poor prognosis in patients with epithelial ovarian cancer. J Clin Oncol 10:95–101PubMedGoogle Scholar
  10. Kono M, Yoshida Y, Kojima N, Tsuji S (1996) Molecular cloning and expression of a fifth-type of α2,8-sialyltransferase (ST8SiaV): its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3. J Biol Chem 271:29366–29371PubMedCrossRefGoogle Scholar
  11. Kono M, Tsuda T, Ogata S, Takashima S, Liu H, Hamamoto T, Itzkowitz SH, Nishimura S, Tsuji S (2000) Re-defined substrate specificity of ST6GalNAc-II: a second candidate sialyl-Tn synthase. Biochem Biophys Res Commun 272:94–97PubMedCrossRefGoogle Scholar
  12. Kurosawa N, Hamamoto T, Lee Y-C, Nakaoka T, Kojima N, Tsuji S (1994) Molecular cloning and expression of GalNAc α2,6-sialyltransferase. J Biol Chem 269:1402–1409PubMedGoogle Scholar
  13. Kurosawa N, Inoue M, Yoshida Y, Tsuji S (1996) Molecular cloning and genomic analysis of mouse Galβl,3GalNAc-specific GalNAc α2,6-sialyltransferase. J Biol Chem 271:15109–15116PubMedCrossRefGoogle Scholar
  14. Kurosawa N, Takashima S, Kono M, Ikehara Y, Inoue M, Tachida Y, Narimatsu H, Tsuji S (2000) Molecular cloning and genomic analysis of mouse GalNAc α2,6-sialyltransferase(ST6GalNAc-I). J Biochem 127:845–854PubMedCrossRefGoogle Scholar
  15. Lee YC, Kaufmann M, Kitazume-Kawaguchi S, Kono M, Takashima S, Kurosawa N, Liu H, Pircher H, Tsuji S (1999) Molecular cloning and functional expression of two members of mouse NeuAcα2,3Galβ1,3GalNAc GalNAcα2,6-sialyltransferase family, ST6GalNAc III and IV. J Biol Chem 274:11958–11967. doi:10.1074/jbc.274.17.11958PubMedCrossRefGoogle Scholar
  16. Leivonen M, Nordling S, Lundin J, von Boguslawski K, Haglund C (2001) STn and prognosis in breast cancer. Oncology 61:299–305PubMedCrossRefGoogle Scholar
  17. Marcos NT, Pinho S, Grandela C, Cruz A, Samyn-Petit B, Harduin-Lepers A, Almeida R, Silva F, Morais V, Costa J, Kihiberg J, Clausen H, Reis CA (2004) Role of human ST6GalNAc-I and ST6GalNAc-II in the synthesis of the cancer-associated Sialyl-Tn antigen. Cancer Res 64:7050–7057PubMedCrossRefGoogle Scholar
  18. Nakagoe T, Sawai T, Tsuji T, Jibiki M-A, Nanashima A, Yamaguchi H, Yasutake T, Ayabe H, Arisawa K, Ishikawa H (2002) Predictive factors for preoperative serum levels of sialyl Lewis(x), sialyl Lewis(a) and sialyl Tn antigens in gastric cancer patients. Anticancer Res 22:451–458PubMedGoogle Scholar
  19. Niwa H, Yamamura K, Miyazaki J (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193–199PubMedCrossRefGoogle Scholar
  20. Ragupathi G, Koide F, Sathyan N, Kagan E, Spassova M, Bornmann W, Gregor P, Reis CA, Clausen H, Danishefsky SJ, Livingston PO (2003) A preclinical study comparing approaches for augmenting the immunogenicity of a heptavalent KLH-conjugate vaccine against epithelial cancers. Cancer Immunol Immunother 52:608–616PubMedCrossRefGoogle Scholar
  21. Sadler JE, Rearick JI, Hill RL (1979) Purification to homogeneity and enzymatic characterization of an a-N-acetylgalactosaminide α2-6 sialyltransferase from porcine sub-maxillary glands. J Biol Chem 254:5934–5941PubMedGoogle Scholar
  22. Samyn-Petit B, Krzewinski-Recchi MA, Steelant WF, Delannoy P, Harduin-Lepers A (2000) Molecular cloning and functional expression of human ST6GalNAc II. Molecular expression in various human cultured cells. Biochim Biophys Acta 1474:201–211PubMedCrossRefGoogle Scholar
  23. Sewell R, Backstrom M, Dalziel M, Gschmeissner S, Karlsson H, Noll T, Gatgens J, Clausen H, Hansson GC, Burchell J, Taylor-Papadimitriou J (2006) The ST6GalNAc-l sialyltransferase localizes throughout the golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer. J Biol Chem 281:3586–3594PubMedCrossRefGoogle Scholar
  24. Takashima S (2008) Characterization of mouse sialyltransferase genes: their evolution and diversity. Biosci Biotechnol Biochem 72:1155–1167PubMedCrossRefGoogle Scholar
  25. Takashima S, Tsuji S (2011) Functional diversity of mammalian sialyltransferases. Trends Glycosci Glycotech 23:178–193. doi:10.4052/tigg.23.178CrossRefGoogle Scholar
  26. Tamura G, Sakata K, Nishizuka S et al (1996) Inactivation of the E-cadherin gene in primary gastric carcinomas and gastric carcinoma cell lines. Jpn J Cancer Res 87:1153–1159PubMedCrossRefGoogle Scholar
  27. Tsuji S (1999) Molecular cloning and characterization of sialyltransferases. In: Inoue Y, Lee YC, Troy FA (eds) Sialobiology and other novel forms of glycosylation. Gakushin, Osaka, pp 145–154Google Scholar
  28. Tsuji S, Datta AK, Paulson JC (1996) Systematic nomenclature for sialyltransferases. Glycobiology 6(7):v–viiPubMedCrossRefGoogle Scholar
  29. Vlad AM, Muller S, Cudic M, Paulsen H, Otvos L Jr, Hanisch F-G, Finn OJ (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigenMUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells. J Exp Med 196:1435–1446PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Institute of Glycoscience, Tokai UniversityHiratsukaJapan
  2. 2.Laboratory of GlycobiologyThe Noguchi InstituteItabashiJapan

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