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Beta-1,3-Glucosyltransferase (B3GALTL)

  • Takashi Sato
  • Hisashi Narimatsu
Reference work entry

Abstract

The B3GALTL gene encodes a beta-1,3-glucosyltransferase (β3Glc-T) that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats (TSRs) of several proteins. Protein O-linked fucosylation is unusual glycosylation associated with important biological functions such as Notch signaling. Two distinct fucosylation pathways synthesizing O-fucosylglycans have been reported on cysteine-knotted core proteins, namely, epidermal growth factor-like (EGF) repeats and TSRs (Fig. 3.1). O-fucosylation on the EGF repeats is catalyzed by POFUT1, which transfers Fuc toward Ser/Thr residues on specific core proteins, and followed by Fringe, which transfers GlcNAc toward Fuc and is essential for Notch activation through its ligands Delta and Serrate/Jagged. This modification is further elongated by reactions of β4GalT and α2,3 or α2,6 SiaT. Another O-fucosylglycan on TSRs is a unique disaccharide structure of Glcβ1,3Fucα1-Ser/Thr synthesized by POFUT2 for the first Fuc and β3Glc-T for the followed Glc, and further elongation has not been identified. Interestingly, O-fucosylation on TSRs occurs in ER, as opposed to that on the EGF repeat, which occurs in ER and the Golgi apparatus. Biological functions of disaccharide of Glcβ1,3Fucα1-TSRs are still remaining unknown; however, a mutation in B3GALTL causes Peters plus syndrome indicating that Glc modification on O-fuc of the TSRs is essential for their function.

Keywords

Acceptor Substrate Ligand Delta Glucosyltransferase Activity Microsomal Membrane Fraction Core Protein Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Japan 2014

Authors and Affiliations

  1. 1.Research Center for Medical GlycoscienceNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan

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