Handbook of Glycosyltransferases and Related Genes pp 1631-1635 | Cite as
Fucose-1-Phosphate Guanylyltransferase (FPGT)
l-fucose is an important monosaccharide in glycosylated proteins. Fucosylation plays a pivotal important role in cell signaling events related to inflammation, fertilization, development, and tumor metastasis programmed cell death (Becker 2003; Luhn 2012). The fucosyl donor for these fucosylated glycans decorating glycoproteins is GDP-beta-l-fucose. Two alternate pathways exist for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-d-mannose to GDP-beta-l-fucose via three enzymatic reactions. The alternative salvage pathway yields GDP-fucose derived directly from fucose. This route utilizes fucose first transported into the cytosol from an extracellular origin or fucose that is liberated from fucosylated glycans in the lysosome and then transported into the cytosol. Two enzymes participate in the salvage pathway; fucose kinase and Fucose-1-phosphate guanylyltransferase (GDP-fucose pyrophosphorylase, FPGT) with fucose-1-phosphate is the metabolic intermediate. Newly synthesized GDP-fucose is then transported into the Golgi lumen where it is used by various fucosyltransferases to generate fucosylated glycans finally decorating glycoproteins and glycolipids (Yurchenco 1977; Pastuszak 1998; Smith 2002; Becker 2003; Luhn 2012).