Mannosyl (Alpha-1,3-)-Glycoprotein Beta-1,4-N-Acetylglucosaminyltransferase, Isozyme A,B (MGAT4A,B)

Reference work entry


Branches of complex-type multi-antennary N-glycan possess various carbohydrate moieties to interact with other molecules and exert micromolecular influences to their carrier proteins and counterpart molecules. Thereby, regulation of the branch formation on N-glycans is important for the maintenance of cellular homeostasis in whole body (Ohtsubo and Marth 2006). Indeed, amount of the fractions of multi-antennary N-glycans of bioactive glycoproteins is altered in response to physiological conditions that modulate their biological activities. The number and complexity of antennae primarily reflect the portfolio of expressed N-acetylglucosaminyltransferases (GnTs) and processing enzymes (Schachter 1991). GnT-IV is an indispensable enzyme producing multi-antennary N-glycans cooperatively with GnT-V. GnT-IV gene family consists of three genes named GnT-IVa, GnT-IVb, and GnT-IV-H, respectively (Minowa et al. 1998; Yoshida et al. 1998; Sakamoto et al. 2000). GnT-IVa and GnT-IVb isoenzymes have GnT-IV enzymatic activity and share substrate specificity with different tissue distribution profiles. In the past decades, the pathophysiological significance of the GnT-IV-dependent production of complex-type multi-antennary N-glycans has been gradually revealed. Attenuation and aberrant induction of GnT-IV enzymatic activity are associated with pathogenesis of grievous diseases.


Acceptor Substrate Branch Formation Double Deficient Mouse Tissue Distribution Profile Human Hepatic Carcinoma 
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© Springer Japan 2014

Authors and Affiliations

  1. 1.Department of Analytical Biochemistry, School of Health SciencesKumamoto UniversityKumamotoJapan
  2. 2.Disease Glycomics Team, Systems Glycobiology Research GroupRIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Global Research Cluster, RIKENWakoJapan

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