General Physiology of Alkaliphiles

  • Koki HorikoshiEmail author
Reference work entry

Generally, alkaliphiles require alkaline environments and sodium ions not only for growth but also for sporulation and germination. Sodium ion-dependent uptake of nutrients has been reported in some alkaliphiles. Many alkaliphiles require various nutrients, such as polypeptone and yeast extracts, for their growth; several alkaliphilic Bacillus strains (Bacillus halodurans C-125, A-59, C-3, and AH-101) can grow in simple minimal media containing glycerol, glutamic acid, citric acid, etc. One of the best strains for genetic analysis is alkaliphilic B. halodurans C-125 and its many mutants have been made by conventional mutation methods. Whole genome sequence was determined and annotated in 2000 (Takami et al. 2000).

Extracellular pH Values

Alkaliphilic microorganisms are ubiquitous: Many alkaliphilic bacteria and archaea can be isolated more commonly from the earth. Alkalinity in nature may be the result of the geology and climate of the area, of industrial processes, or promoted by...


Alkaliphilic Bacillus Flagellar Motor Alkaline Protease Producer Alkaliphilic Bacterium Teichuronic Acid 
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  1. Aono R (1985) Isolation and partial characterization of structural components of the cell walls of alkalophilic Bacillus strain C-125. J Gen Microbiol 131:105–111Google Scholar
  2. Aono R (1987) Characterization of structural component of cell walls of alkalophilic strain of Bacillus sp. C-125: preparation of poly(γ-L-glutamate) from cell wall component. Biochem J 245:467–472PubMedGoogle Scholar
  3. Aono R (1989) Characterization of cell wall components of the alkalophilic Bacillus strain C-125: identification of a polymer composed of polyglutamate and polyglucuronate. J Gen Microbiol 135:265–271Google Scholar
  4. Aono R (1990) The poly-α- and -β-1, 4-glucuronic acid moiety of teichuronopeptide from the cell wall of the alkalophilic Bacillus strain C-125. Biochem J 270:363–367PubMedGoogle Scholar
  5. Aono R, Horikoshi K (1983) Chemical composition of cell walls of alkalophilic strains of Bacillus. J Gen Microbiol 129:1083–1087Google Scholar
  6. Aono R, Uramot M (1986) Presence of fucosamine in teichuronic acid of alkalophilic Bacillus strain C-125. Biochem J 233:291–294PubMedGoogle Scholar
  7. Aono R, Ogino H, Horikoshi K (1992) pH-dependent flagella formation by facultative alkaliphilic Bacillus sp. C-125. Biosci Biotechnol Biochem 56:48–53PubMedCrossRefGoogle Scholar
  8. Aono R, Ito M, Horikoshi K (1993) Occurrence of teichuronopeptide in cell walls of Group-2 Alkaliphilic Bacillus spp. J Gen Microbiol 139(Part 11):2739–2744Google Scholar
  9. Aono R, Ito M, Joblin K, Horikoshi K (1994) Genetic recombination after cell fusion of protoplasts from the facultative alkaliphile Bacillus sp. C-125. -Uk Microbiology. 140(Part 11):3085–3090,CrossRefGoogle Scholar
  10. Aono R, Ito M, Joblin KN, Horikoshi K (1995) A high cell wall negative charge is necessary for the growth of the alkaliphile Bacillus lentus C-125 at elevated pH. -Uk Microbiology 141(Part 11):2955–2964CrossRefGoogle Scholar
  11. Aono R, Ito M, Horikoshi K (1997) Measurement of cytoplasmic pH of the alkaliphile Bacillus lentus C-125 with a fluorescent pH probe. Uk Microbiology 143:2531–2536CrossRefGoogle Scholar
  12. Aono R, Ito M, Machida T (1999) Contribution of the cell wall component teichuronopeptide to pH homeostasis and alkaliphily in the alkaliphile Bacillus lentus C-125. J Bacteriol 181:6600–6606PubMedGoogle Scholar
  13. Ashiuchi M, Misono H (2002) Biochemistry and molecular genetics of poly-γ-L-glutamate synthesis. Appl Microbiol Biotechnol 59:9–14PubMedCrossRefGoogle Scholar
  14. Boyer EW, Ingle MB, Mercer GD (1973) Bacillus alcalophilus subsp. halodurans subsp. nov.: an alkaline-amylase-producing alkalophilic organisms. Int J Syst Bacteriol 23:238–242CrossRefGoogle Scholar
  15. Gilmour R, Messner P, Guffanti AA, Kent R, Scheberl A, Kendrick N, Krulwich TA (2000) Two-dimensional gel electrophoresis analyses of pH-dependent protein expression in facultatively alkaliphilic Bacillus pseudofirmus OF4 lead to characterization of an S-layer protein with a role in alkaliphily. J Bacteriol 182:5969–5981PubMedCrossRefGoogle Scholar
  16. Guffanti AA, Susman P, Blanco R, Krulwich TA (1978) The proton-motive force and α-aminoisobutyric acid transport in an obligatory alkalophilic bacterium. J Biol Chem 253:708–715PubMedGoogle Scholar
  17. Hamamoto T, Hashimoto M, Hino M, Kitada M, Seto Y, Kudo T, Horikoshi K (1994) Characterization of a gene responsible for the Na+/H+ antiporter system of alkalophilic Bacillus species strain C-125. Mol Microbiol 14:939–946PubMedCrossRefGoogle Scholar
  18. Hashimoto M, Hamamoto T, Kitada M, Hino M, Kudo T, Horikoshi K (1994) Characteistics of alkali-sensitive mutants of alkaliphilic Bacillus sp. strain C-125 that show cellular morphological abnormalities. Biosci Biotechnol Biochem 58:2090–2092CrossRefGoogle Scholar
  19. Hiramatsu T, Kodama K, Kuroda T, Mizushima T, Tsuchiya T (1998) A putative multisubunit Na+/H+ antiporter from Staphylococcus aureus. J Bacteriol 180:6642–6648PubMedGoogle Scholar
  20. Hirota M, Kitada M, Imae Y (1981) Flagellar motors of alkalophilic Bacillus are powered by an electrochemical potential gradient of Na+. FEBS Lett 132:278–280CrossRefGoogle Scholar
  21. Horikoshi K (1971) Production of alkaline enzymes by alkalophilic microorganisms. Part I. alkaline protease produced by Bacillus no. 221. Agric Biol Chem 36:1407–1414CrossRefGoogle Scholar
  22. Horikoshi K, Iida S (1958) Lysis of fungal mycelia by bacterial enzymes. Nature 181:917–918PubMedCrossRefGoogle Scholar
  23. Horikoshi K, Yonezawa Y (1978) A bacteriophage active on an alkalophilic Bacillus sp. J Gen Virol 39:183–185CrossRefGoogle Scholar
  24. Ikura Y, Horikoshi K (1978) Cell free protein synthesizing system of alkalophilic Bacillus No.A-59. Agric Biol Chem 42:753–756CrossRefGoogle Scholar
  25. Ikura Y, Horikoshi K (1983) Studies on cell wall of alkalophilic Bacillus. Agric Biol Chem 47:681–686CrossRefGoogle Scholar
  26. Ito M, Nagane M (2001) Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment. Biosci Biotechnol Biochem 65:2773–2775PubMedCrossRefGoogle Scholar
  27. Ito M, Guffanti AA, Oudega B, Krulwich TA (1999) Mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis. J Bacteriol 181:2394–2402PubMedGoogle Scholar
  28. Ito M, Guffanti AA, Wang W, Krulwich TA (2000) Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na+ and alkali but not cholate resistance. J Bacteriol 182:5663–5670PubMedCrossRefGoogle Scholar
  29. Ito M, Guffanti AA, Krulwich TA (2001) Mrp-dependent Na+/H+ antiporters of Bacillus exhibit characteristics that are unanticipated for completely secondary active transporters. FEBS Lett 496(2–3):117–120PubMedCrossRefGoogle Scholar
  30. Ito M, Hicks DB, Henkin TM, Guffanti AA, Powers BD, Zvi L, Uematsu K, Krulwich TA (2004) MotPS is the stator-force generator for motility of alkaliphilic Bacillus, and its homologue is a second functional Mot in Bacillus subtilis. Mol Microbiol 53:1035–1049PubMedCrossRefGoogle Scholar
  31. Jarrell KF, Vydykhan T, Lee P, Agnew MD, Thomas NA (1997) Isolation and characterization of bacteriophage BCJA1, a novel temperate bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii. Extremophiles 1:199–206PubMedCrossRefGoogle Scholar
  32. Kimura T, Horikoshi K (1988) Isolation of bacteria which can grow at both high pH and low temperature. Appl Environ Microbiol 54:1066–1067PubMedGoogle Scholar
  33. Kitada M, Horikoshi K (1977) Sodium ion-stimulated α-(1-14C)-aminoisobutyric acid uptake in alkalophilic Bacillus species. J Bacteriol 131:784–788PubMedGoogle Scholar
  34. Kitada M, Kosono S, Kudo T (2000) The Na+/H+ antiporter of alkaliphilic Bacillus sp. Extremophiles 4:253–258PubMedCrossRefGoogle Scholar
  35. Kojima S, Asai Y, Atsumi T, Kawagishi I, Homma M (1999) Na+-driven flagellar motor resistant to phenamil, an amiloride analog, caused by mutations in putative channel components. J Mol Biol 285:1537–1547PubMedCrossRefGoogle Scholar
  36. Kosono S, Asai K, Sadaie Y, Kudo T (2004) Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis. FEMS Microbiol Lett 232:93–99PubMedCrossRefGoogle Scholar
  37. Koyama N, Nosoh Y (1976) Effect of the pH of culture medium on the alkalophilicity of a species of Bacillus. Arch Microbiol 109:105–108PubMedCrossRefGoogle Scholar
  38. Koyama N, Kiyomiya A, Nosoh Y (1976) Na+ -dependent uptake of amino acids by an alkalophilic Bacillus. FEBS Lett 72:77–78PubMedCrossRefGoogle Scholar
  39. Kropinski A, Hayward M, Agnew MD, Jarrell KF (2005) The genome of BCJA1c: a bacteriophage active against the alkaliphilic bacterium, Bacillus clarkii. Extremophiles 9:99–109PubMedCrossRefGoogle Scholar
  40. Krulwich TA, Ito M, Guffanti AA (2001) The Na+-dependence of alkaliphily in Bacillus. Biochim Biophys Acta 1505:158–168PubMedCrossRefGoogle Scholar
  41. Kudo T, Horikoshi K (1979) The environmental factors affecting sporulation of an alkalophilic Bacillus species. Agric Biol Chem 43:2613–2614CrossRefGoogle Scholar
  42. Kudo T, Horikoshi K (1983a) Effect of pH and sodiium ion on germination of alkalophilic Bacillus species. Agric Biol Chem 47:665–669CrossRefGoogle Scholar
  43. Kudo T, Horikoshi K (1983b) The effect of pH on heat-resistance of spores of alkalophilic Bacillus no. 2b-2. Agric Biol Chem 47:403–404CrossRefGoogle Scholar
  44. Kudo T, Hino M, Kitada M, Horikoshi K (1990) DNA sequences required for the alkalophily of Bacillus sp. strain C-125 are located close together on its chromosomal DNA. J Bacteriol 172:7282–7283PubMedGoogle Scholar
  45. Kurono Y, Horikoshi K (1973) Alkaline catalase produced Bacillus no. Ku-1. Agric Biol Chem 37:2565–2570CrossRefGoogle Scholar
  46. Roadcap GS, Kelly WR, Bethke CM (2005) Geochemistry of extremely alkaline (pH >12) ground water in slag-fill aquifers. Ground Water 43:806–816PubMedCrossRefGoogle Scholar
  47. Roadcap GS, Sanford RA, Jin Q, Pardinas JR, Bethke CM (2006) Extremely alkaline (pH >12) ground water hosts diverse microbial community. Ground Water 44:511–517PubMedCrossRefGoogle Scholar
  48. Sakamoto Y, Sutherland KJ, Tamaoka J, Kobayashi T, Kudo T, Horikoshi K (1992) analysis of the flagellin (hag) gene of alkalophilic Bacillus sp. C-125. J Gen Micriobiol 138:2139–2166Google Scholar
  49. Seto Y, Hashimoto M, Usami R, Hamamoto T, Kudo T, Horikoshi K (1995) Characterization of a mutation responsible for an alkali-sensitive mutant, 18224, of alkaliphilic Bacillus sp. strain C-125. Biosci Biotechnol Biochem 59:1364–1366PubMedCrossRefGoogle Scholar
  50. Sugiyama S, Matsukura H, Koyama N, Nosoh Y, Imae Y (1986) Requirement of Na+ in flagellar rotation and amino-acid transport in a facultatively alkalophilic Bacillus. Biochim Biophys Acta 852:38–45CrossRefGoogle Scholar
  51. Swartz TH, Ikewada S, Ishikawa O, Ito M, Krulwich TA (2005a) The Mrp system: a giant among monovalent cation/proton antiporters? Extremophiles 9:345–354PubMedCrossRefGoogle Scholar
  52. Swartz TH, Ito M, Hicks DB, Nuqui M, Guffanti AA, Krulwich TA (2005b) The Mrp Na+/H+ antiporter increases the activity of the malate:quinone oxidoreductase of an Escherichia coli respiratory mutant. J Bacteriol 187:388–391PubMedCrossRefGoogle Scholar
  53. Takami H, Nakasone K, Hirama C, Takaki Y, Masui N, Fuji F, Nakamura Y, Inoue A (1999) An improved physical and genetic map of the genome of alkaliphilic Bacillus sp. C-125. Extremophiles 3:21–28PubMedCrossRefGoogle Scholar
  54. Takami H, Nakasone K, Takaki Y, Maeno G, Sasaki R, Masui N, Fuji F, Hirama C, Nakamura Y, Ogasawaral N, Kuhara S, Horikoshi K (2000) Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res 28:4317–4331PubMedCrossRefGoogle Scholar
  55. Tsujii K (2002) Donnan equilibrate cell walls: a pH-homeostasis mechanism in alkaliphiles. Colloids Surf B Biointerfaces 24:247CrossRefGoogle Scholar
  56. Wei Y, Southworth TW, Kloster H, Ito M, Guffanti AA, Moir A, Krulwich TA (2003) Mutational loss of a K+ and NH4+ transporter affects the growth and endospore formation of alkaliphilic Bacillus pseudofirmus OF4. J Bacteriol 185:5133–5147PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2011

Authors and Affiliations

  1. 1.Japan Agency for Marine-Earth Science and Technology (JAMSTEC)YokohamaJapan

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