Molecular Responses to Solvent Stress: Strategies for Living in Unpalatable Substrates
This review article covers some general conclusions reached in research studies with respect to a number of solvent-tolerant Pseudomonas strains. The seminal observation was performed by Inoue and Korikoshi 1989 when they described in Nature a bacterium, belonging to the genus Pseudomonas, which was able to thrive in the presence of high concentrations of toluene. This property of extreme tolerance to solvents makes this Pseudomonas strain the first extremophile identified as able to survive in the presence of highly toxic solvents. Following this seminal observation, other solvent-tolerant strains, such as Pseudomonas putida DOT-T1E, S12, GM1, and MTB6, were described as able to grow in the presence of highly toxic solvents such as p-xylene (log Pow 3.15), styrene (log Pow 3.0), octanol (log Pow 2.92), and toluene (log Pow 2.69) (Aono et al. 1992; Cruden et al. 1992; Huertas et al. 2000; Isken and de Bont 1996; Kim et al. 1998; Ramos et al. 1995; Weber et al. 1994)....
KeywordsEfflux Pump Outer Membrane Protein Cyclopropane Fatty Acid Solvent Tolerance Toluene Degradation
Work in our laboratory was supported by grants BIO2008-04419-E, Consolider-Ingenio (CSD2007-00005) of the Spanish Ministry, CVI-3010 from Junta de Andalucia and BACSIN (FP7-KBBE-2007-1) from the European Commission.
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