Index of Solvent Toxicity to Microorganisms
The results of our investigation of solvent tolerance clarified that the growth of Pseudomonas putida strain IH-2000 differed depending on the organic solvent added to the culture medium. To determine how organic solvents affect the growth of microorganisms, the structural correlation between growth and the type of organic solvent was examined.
The physical basis of solvent toxicity is still poorly understood, and no physical parameter has been identified by which the relative toxicity of different solvents can be determined. Consequently, the solvent tolerance of microorganisms has not yet been correlated with organic solvent type. To investigate the correlation between the solvent tolerance of microorganisms and solvent toxicity, we isolated solvent-sensitive mutants using the replica-plating method in conjunction with penicillin selection after culture with 1-methyl-3-nitro-1-nitrosoguanidine (NTG). First, we constructed a Leu and Trp...
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References
Abe A, Inoue A, Usami R, Moriya K, Horikoshi K (1995) Properties of newly isolated marine bacterium that can degrade polyaromatic hydrocarbons in the presence of organic solvents. J Mar Biotechnol 2:182–186
Aono R, Aibe K, Inoue A, Horikoshi K (1991) Preparation of organic solvent-tolerant mutants from Escherichia coli K-12. Agric Biol Chem 55:1935–1938
Aono R, Ito M, Inoue A, Horikoshi K (1992) Isolation of novel toluene-tolerant strain of Pseudomonas aeruginosa. Biosci Biotechnol Biochem 56:145–146
Bocren S, Laane C (1987) Steroid conversions by Flavobabterium dehydrogenans in two-liquid-phase systems. Biotechnol Bioeng 29:300–305
Corwin H, Anderson M (1967) The effect of intramolecular hydrophobic bonding on partition coefficients. J Org Chem 32:2583–2586
Flygare S, Larsson P (1987) Steroid transformation using magnetically immobilized Mycobacterium sp. Enzyme Microb Technol 9:494–499
Fukumaki T, Inoue A, Moriya K, Horikoshi K (1994) Isolation of marine yeast that degrades hydrocarbon in the presence of organic solvent. Biosci Biotechnol Biochem 58:1784–1788
Hansch C, Fujita T (1964) A method for the correlation of biological activity and chemical structure. J Am Chem Soc 86:1616–1626
Hansch C, Muir MR, Fujita T, Maloney PP, Geiger F, Streich M (1963) The correlation of biological activity of plant growth regulators and chloromycetin derivatives with hammett constants and partition coefficients. J Am Chem Soc 85:2817–2824
Harnish M, Mocket HJ, Schulze GJ (1983) Relationship between log Pow shake-flask values and capacity factors derived from reversed phase high-performance liquid chromatography for n-alkylbenzenes and some oecd reference substance. J Chromatogr 282:315–332
Inoue A, Horikoshi K (1989) A Pseudomonas thrives in high concentrations of toluene. Nature 338:264–266
Inoue A, Horikoshi K (1991a) Estimation of solvent-tolerance of bacteria by the solvent parameter log P. J Ferment Bioeng 71:194–196
Inoue A, Horikoshi K (1991b) Pseudo, honas putida which can grow in the presence of toluene. Appl Environ Microbiol 57:1560–1562
Jones D, Collins DM (1984) Irregular, nonsporing gram-positive rods. In: Bergey’s manual of systematic bacteriology, vol 2. Williams & Wilkins, Baltimore, pp 1261–1434
Krieg NR, Holt JG (1984) Gram-negative aerobic rods and cocci. In: Murray RGE, Breuner DJ (eds) Bergey’s manyal of systematic bacteriology, vol I. Williams & Wilkins, Baltimore, p 140
Moriya K, Horikoshi K (1993a) Isolation of a benzene-tolerant bacterium and its hydrocarbon degradation. J Ferment Bioeng 76:168–173
Moriya K, Horikoshi K (1993b) A benzene-tolerant bacterium utilizing sulfur compounds isolated from deep-sea. J Ferment Bioeng 76:397–399
Moriya K, Yanagitani S, Usami R, Horikoshi K (1995) Isolation and some properties of an organic-solvent tolerant marine bacterium degrading cholesterol. J Mar Biotechnol 2:131–133
Nakajima H, Kobayashi H, Aono R, Horikoshi K (1873) (1992) Effective isolation and identification of toluene-tolerant Pseudomonas strains. Biosci Biotechnol Biochem 56:1872
Rekker RF, de Kort HM (1979) The hydrophobic fragmental constant; an extension to a 1000 data point set. Eur J Med Chem 14:479–488, Therapeut
Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
Walker JD, Colwell RR (1974) Microbial petroleum degradation: use of mixed hydrocarbon substrates. Appl Environ Microbiol 7:I 053–I 060
Walker JD, Colwell RR, Hamming MC, Ford HT (1975) Extraction of petroleum hydrocarbons from oil-contaminated sediments. Bull Environ Contam Toxicol 13:245–248
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Inoue, A. (2011). Diversity and Ecology of Organic Solvent Tolerant Microorganisms. In: Horikoshi, K. (eds) Extremophiles Handbook. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53898-1_46
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DOI: https://doi.org/10.1007/978-4-431-53898-1_46
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