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Diversity and Ecology of Organic Solvent Tolerant Microorganisms

  • Akira Inoue
Reference work entry

Index of Solvent Toxicity to Microorganisms

The results of our investigation of solvent tolerance clarified that the growth of Pseudomonas putida strain IH-2000 differed depending on the organic solvent added to the culture medium. To determine how organic solvents affect the growth of microorganisms, the structural correlation between growth and the type of organic solvent was examined.

The physical basis of solvent toxicity is still poorly understood, and no physical parameter has been identified by which the relative toxicity of different solvents can be determined. Consequently, the solvent tolerance of microorganisms has not yet been correlated with organic solvent type. To investigate the correlation between the solvent tolerance of microorganisms and solvent toxicity, we isolated solvent-sensitive mutants using the replica-plating method in conjunction with penicillin selection after culture with 1-methyl-3-nitro-1-nitrosoguanidine (NTG). First, we constructed a Leu and Trp...

Keywords

Organic Sulfur Compound Solvent Tolerance Viable Cell Concentration Hydrocarbon Degrader Yeast Nitrogen Base Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Abe A, Inoue A, Usami R, Moriya K, Horikoshi K (1995) Properties of newly isolated marine bacterium that can degrade polyaromatic hydrocarbons in the presence of organic solvents. J Mar Biotechnol 2:182–186Google Scholar
  2. Aono R, Aibe K, Inoue A, Horikoshi K (1991) Preparation of organic solvent-tolerant mutants from Escherichia coli K-12. Agric Biol Chem 55:1935–1938CrossRefGoogle Scholar
  3. Aono R, Ito M, Inoue A, Horikoshi K (1992) Isolation of novel toluene-tolerant strain of Pseudomonas aeruginosa. Biosci Biotechnol Biochem 56:145–146CrossRefGoogle Scholar
  4. Bocren S, Laane C (1987) Steroid conversions by Flavobabterium dehydrogenans in two-liquid-phase systems. Biotechnol Bioeng 29:300–305Google Scholar
  5. Corwin H, Anderson M (1967) The effect of intramolecular hydrophobic bonding on partition coefficients. J Org Chem 32:2583–2586CrossRefGoogle Scholar
  6. Flygare S, Larsson P (1987) Steroid transformation using magnetically immobilized Mycobacterium sp. Enzyme Microb Technol 9:494–499CrossRefGoogle Scholar
  7. Fukumaki T, Inoue A, Moriya K, Horikoshi K (1994) Isolation of marine yeast that degrades hydrocarbon in the presence of organic solvent. Biosci Biotechnol Biochem 58:1784–1788CrossRefGoogle Scholar
  8. Hansch C, Fujita T (1964) A method for the correlation of biological activity and chemical structure. J Am Chem Soc 86:1616–1626CrossRefGoogle Scholar
  9. Hansch C, Muir MR, Fujita T, Maloney PP, Geiger F, Streich M (1963) The correlation of biological activity of plant growth regulators and chloromycetin derivatives with hammett constants and partition coefficients. J Am Chem Soc 85:2817–2824CrossRefGoogle Scholar
  10. Harnish M, Mocket HJ, Schulze GJ (1983) Relationship between log Pow shake-flask values and capacity factors derived from reversed phase high-performance liquid chromatography for n-alkylbenzenes and some oecd reference substance. J Chromatogr 282:315–332CrossRefGoogle Scholar
  11. Inoue A, Horikoshi K (1989) A Pseudomonas thrives in high concentrations of toluene. Nature 338:264–266CrossRefGoogle Scholar
  12. Inoue A, Horikoshi K (1991a) Estimation of solvent-tolerance of bacteria by the solvent parameter log P. J Ferment Bioeng 71:194–196CrossRefGoogle Scholar
  13. Inoue A, Horikoshi K (1991b) Pseudo, honas putida which can grow in the presence of toluene. Appl Environ Microbiol 57:1560–1562PubMedGoogle Scholar
  14. Jones D, Collins DM (1984) Irregular, nonsporing gram-positive rods. In: Bergey’s manual of systematic bacteriology, vol 2. Williams & Wilkins, Baltimore, pp 1261–1434Google Scholar
  15. Krieg NR, Holt JG (1984) Gram-negative aerobic rods and cocci. In: Murray RGE, Breuner DJ (eds) Bergey’s manyal of systematic bacteriology, vol I. Williams & Wilkins, Baltimore, p 140Google Scholar
  16. Moriya K, Horikoshi K (1993a) Isolation of a benzene-tolerant bacterium and its hydrocarbon degradation. J Ferment Bioeng 76:168–173CrossRefGoogle Scholar
  17. Moriya K, Horikoshi K (1993b) A benzene-tolerant bacterium utilizing sulfur compounds isolated from deep-sea. J Ferment Bioeng 76:397–399CrossRefGoogle Scholar
  18. Moriya K, Yanagitani S, Usami R, Horikoshi K (1995) Isolation and some properties of an organic-solvent tolerant marine bacterium degrading cholesterol. J Mar Biotechnol 2:131–133Google Scholar
  19. Nakajima H, Kobayashi H, Aono R, Horikoshi K (1873) (1992) Effective isolation and identification of toluene-tolerant Pseudomonas strains. Biosci Biotechnol Biochem 56:1872CrossRefGoogle Scholar
  20. Rekker RF, de Kort HM (1979) The hydrophobic fragmental constant; an extension to a 1000 data point set. Eur J Med Chem 14:479–488, TherapeutGoogle Scholar
  21. Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  22. Walker JD, Colwell RR (1974) Microbial petroleum degradation: use of mixed hydrocarbon substrates. Appl Environ Microbiol 7:I 053–I 060Google Scholar
  23. Walker JD, Colwell RR, Hamming MC, Ford HT (1975) Extraction of petroleum hydrocarbons from oil-contaminated sediments. Bull Environ Contam Toxicol 13:245–248PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2011

Authors and Affiliations

  1. 1.Graduate School of Interdisciplinary New ScienceToyo UniversityKawagoeJapan
  2. 2.Graduate School of Life SciencesToyo UniversityItakura-machi, Oura-gunJapan
  3. 3.Bio-Nano Electronic Reserch CenterToyo UniversityKawagoeJapan

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