Diversity and Ecology of Organic Solvent Tolerant Microorganisms
Index of Solvent Toxicity to Microorganisms
The results of our investigation of solvent tolerance clarified that the growth of Pseudomonas putida strain IH-2000 differed depending on the organic solvent added to the culture medium. To determine how organic solvents affect the growth of microorganisms, the structural correlation between growth and the type of organic solvent was examined.
The physical basis of solvent toxicity is still poorly understood, and no physical parameter has been identified by which the relative toxicity of different solvents can be determined. Consequently, the solvent tolerance of microorganisms has not yet been correlated with organic solvent type. To investigate the correlation between the solvent tolerance of microorganisms and solvent toxicity, we isolated solvent-sensitive mutants using the replica-plating method in conjunction with penicillin selection after culture with 1-methyl-3-nitro-1-nitrosoguanidine (NTG). First, we constructed a Leu and Trp...
KeywordsOrganic Sulfur Compound Solvent Tolerance Viable Cell Concentration Hydrocarbon Degrader Yeast Nitrogen Base Medium
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