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Versatile Solidified Media for Growth of Extremophiles

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Extremophiles Handbook

Introduction

Solidified media are indispensable in various aspects of microbiological research (Codner 1969; Madigan et al. 1997; Zengler 2009). For solid cultures of mesophilic microorganisms, agar is commonly used as a solidifying agent. It is a nonionic polysaccharide consisting mainly of d-galactose and 3,6-anhydro-l-galactose and is produced by sea-weeds (Lahaye and Rochas 1991). Ease of the handling, resistance to enzymatic degradation by most microorganisms, and transparency of solidified plates make agar an ideal solidifying agent (Hashsham 2007), and agar-supported media have been used essentially unchanged since they were first introduced in the late nineteenth century (Codner 1969; Madigan et al. 1997; Zengler 2009).

The situation is very different when it comes to culturing extremophiles on solidified media. For example, agar media are not suitable for culturing thermophiles and hyperthermophiles because the solidification of agar is thermoreversible at around 50–60°C...

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Authors and Affiliations

  1. Soft Matter and Extremophiles Research Team, Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan

    Dr. Shigeru Deguchi

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  1. Dr. Shigeru Deguchi
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Correspondence to Shigeru Deguchi .

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Editors and Affiliations

  1. Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Yokohama, Professor Emeritus of Tokyo Institute of Technology, Tokyo, Japan

    Koki Horikoshi

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Deguchi, S. (2011). Versatile Solidified Media for Growth of Extremophiles. In: Horikoshi, K. (eds) Extremophiles Handbook. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53898-1_36

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