Cultivation Methods for Piezophiles
Some extremophiles, living under the deep-ocean floor, are microorganisms that have adapted to a high-pressure environment and can grow more easily under high hydrostatic pressure conditions than at atmospheric pressure. We call such deep-sea extremophiles “piezophiles,” meaning pressure- (piezo- in Greek) loving (-phile) organisms (Yayanos 1995). In the laboratory, piezophiles could be isolated and cultivated under high-pressure conditions, and therefore special high-pressure equipments, such as pressure vessels, hydrostatic pumps, etc., are necessary. When sampling was performed from deep-sea sediments or seawater to obtain living piezophiles, we also need a pressure-retaining sampler to maintain the environmental pressure and temperature, because some piezophiles are sensitive to drastic pressure and temperature changes. The Japan Agency for Marine-Earth Science and Technology (JAMSTEC) has been developing a “deep-sea baro-piezophile and thermophile isolation and cultivation...
KeywordsMixed Cultivation Pressure Vessel Environmental Pressure Cultivation Device Philippine Trench
I am very grateful to Prof. Koki Horikoshi for continued support of my extremophile studies. I also thank my colleagues for excellent collaboration. I especially thank Prof. Richard Y. Morita for providing the valuable traditional pressure vessels to study piezophiles, and for his encouragement during the course of high-pressure research. I also thank the submersible operation teams and the DEEPBATH operation staffs for making it possible to undertake piezophilic studies.
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