Advertisement

Cultivation Methods for Piezophiles

  • Chiaki Kato

Some extremophiles, living under the deep-ocean floor, are microorganisms that have adapted to a high-pressure environment and can grow more easily under high hydrostatic pressure conditions than at atmospheric pressure. We call such deep-sea extremophiles “piezophiles,” meaning pressure- (piezo- in Greek) loving (-phile) organisms (Yayanos 1995). In the laboratory, piezophiles could be isolated and cultivated under high-pressure conditions, and therefore special high-pressure equipments, such as pressure vessels, hydrostatic pumps, etc., are necessary. When sampling was performed from deep-sea sediments or seawater to obtain living piezophiles, we also need a pressure-retaining sampler to maintain the environmental pressure and temperature, because some piezophiles are sensitive to drastic pressure and temperature changes. The Japan Agency for Marine-Earth Science and Technology (JAMSTEC) has been developing a “deep-sea baro-piezophile and thermophile isolation and cultivation...

Keywords

Mixed Cultivation Pressure Vessel Environmental Pressure Cultivation Device Philippine Trench 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I am very grateful to Prof. Koki Horikoshi for continued support of my extremophile studies. I also thank my colleagues for excellent collaboration. I especially thank Prof. Richard Y. Morita for providing the valuable traditional pressure vessels to study piezophiles, and for his encouragement during the course of high-pressure research. I also thank the submersible operation teams and the DEEPBATH operation staffs for making it possible to undertake piezophilic studies.

References

  1. Abe F, Horikoshi K (1998) Analysis of intracellular pH in the yeast Saccharomyces cerevisiae under elevated hydrostatic pressure: a study in baro- (piezo-) physiology. Extremophiles 2:223–228PubMedCrossRefGoogle Scholar
  2. Akasaka K (2006) Probing conformational fluctuation of proteins by pressure perturbation. Chem Rev 106:1814–1835PubMedCrossRefGoogle Scholar
  3. Kato C, Sato T, Horikoshi K (1995) Isolation and properties of barophilic and barotolerant bacteria from deep-sea mud samples. Biodivers Conserv 4:1–9CrossRefGoogle Scholar
  4. Kato C, Nakasone K, Qureshi MH, Horikoshi K (2000) How do deep-sea microorganisms respond to the environmental pressure? In: Storey KB, Storey JM (eds) Cell and molecular response to stress, vol 1, Environmental stressors and gene responses. Elsevier, Amsterdam, pp 277–291Google Scholar
  5. Kato C, Li L, Nogi Y, Nakasone K, Bartlett DH (2001) 10: Marine microbiology: deep sea adaptations. In: Taniguchi Y, Stanley HE, Ludwig H (eds) Biological systems under extreme conditions, structure and function, Biological and medical physics series. Springer, Heidelberg, pp 205–220Google Scholar
  6. Kato C, Sato T, Nogi Y, Nakasone K (2004) Piezophiles: high pressure-adapted marine bacteria. Mar Biotechnol 6:s195–s201Google Scholar
  7. Kato C, Nogi Y, Arakawa S (2008) Chapter 12. Isolation, Cultivation, and Diversity of Deep-Sea Piezophiles. In: High-Pressure Microbiology, Michiels C, Bartlett DH, Aertsen A (eds), ASM press, Washington DC, pp. 203–217.Google Scholar
  8. Kawano H, Nakasone K, Matsumoto M, Usami R, Kato C, Abe F (2004) Differential pressure resistance in the activity of RNA polymerase isolated from Shewanella violacea and Escherichia coli. Extremophiles 8:367–375PubMedCrossRefGoogle Scholar
  9. Kyo M, Tuji T, Usui H, Itoh T (1991) Collection, isolation and cultivation system for deep-sea microbes study: concept and design. Oceans 1:419–423Google Scholar
  10. Marquis RE (1976) High pressure microbial physiology. Adv Microb Physiol 14:159–241PubMedCrossRefGoogle Scholar
  11. Miwa T, Sato T, Kato C, Aizawa M, Horikoshi K (2002) Restoration of Escherichia coli from high hydrostatic pressure – A study of the FtsZ-ring formation using confocal laser microscopy. In: Hayashi R (ed) Progress in biotechnology 19, Trends in high pressure bioscience and biotechnology, pp 227–231Google Scholar
  12. Nakasone K, Ikegami A, Kato C, Usami R, Horikoshi K (1998) Mechanisms of gene expression controlled by pressure in deep-sea microorganisms. Extremophiles 2:149–154PubMedCrossRefGoogle Scholar
  13. Nakasone K, Ikegami A, Kawano H, Usami R, Kato C, Horikoshi K (2002) Transcriptional regulation under pressure conditions by the RNA polymerase σ54 factor with a two component regulatory system in Shewanella violacea. Extremophiles 6:89–95PubMedCrossRefGoogle Scholar
  14. Yayanos AA (1995) Microbiology to 10,500 meters in the deep sea. Annu Rev Microbiol 49:777–805PubMedCrossRefGoogle Scholar
  15. Yayanos AA, Dietz AS, Boxtel RV (1979) Isolation of a deep-sea barophilic bacterium and some of its growth characteristics. Science 205:808–810PubMedCrossRefGoogle Scholar
  16. Zobell CE, Morita RY (1957) Barophilic bacteria in some deep-sea sediments. J Bacteriol 73:563–568PubMedGoogle Scholar

Copyright information

© Springer 2011

Authors and Affiliations

  1. 1.Institute of BiogeosciencesJapan Agency for Marine-Earth Science and Technology (JAMSTEC)YokosukaJapan

Personalised recommendations