Genetics of Thermophiles


Increasing numbers of genomic sequence of thermophiles have been reported so far. In the so-called post-genomic era, much effort has been made for uncovering biological meaning hidden in the genomic context, which is a great challenge in computational biology. In addition to the computer-based analysis, a lot of gene products are analyzed in vitro with the recombinant proteins produced in a mesophile such as Escherichia coli taking advantage of the available nucleotide sequence information. The recombinant proteins from thermophiles can easily be purified by heat treatment to remove the heat-labile mesophilic proteins, followed by column chromatography if necessary. Many of the crystal structures of proteins revealed to date are derived from thermophiles.

Sometimes, however, it is difficult to predict a gene function within a cell by in silico analysis. Genetic manipulation system for the native thermophile host is one of the valuable methods to overcome the problem. In...


Selection Marker Insertional Mutagenesis Cryptic Plasmid lacS Gene Orotate Phosphoribosyl Transferase 


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© Springer 2011

Authors and Affiliations

  1. 1.Department of Molecular BiologyTokyo University of Pharmacy and Life SciencesHachiojiJapan
  2. 2.Institute of Environmental MicrobiologyKyowa-kako Co.MachidaJapan

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