Preparation of Anthracyclines Liposomes for Tumor-Targeting Drug Delivery
Anthracyclines are the most effective anticancer drugs with broad cancer spectrum. They demonstrate strong anticancer efficacy in vitro but are less effective in vivo during treatment of brain cancer due to the hindrance of the blood-brain barrier (BBB). In this regard, this protocol focuses on the fabrication of dual-ligand modified anthracycline liposomes for treatment of brain cancer through transferring drug across the BBB and then for targeting brain cancer. Here, the preparation and characterization techniques of dual-targeting daunorubicin liposomes are described. In this construct, daunorubicin is used as a model drug, while all anthracyclines could be loaded into the liposomes with the same procedures. The present results demonstrate that the dual-targeting daunorubicin liposomes exhibit a high drug encapsulation efficiency (>90%), an increased transport of the drug liposomes across the BBB, and then a targeted effect in killing brain glioma cells, thereby improving the therapeutic efficacy of brain glioma in vitro and in animals.
KeywordsAnthracyclines Dual -targeting Liposomes Blood-brain barrier Brain glioma-bearing rats Survival
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