Encyclopedia of Membranes

2016 Edition
| Editors: Enrico Drioli, Lidietta Giorno

Fluorescence Excitation-Emission Matrix (EEM)

  • Carla A. M. Portugal
  • Claudia F. Galinha
Reference work entry
DOI: https://doi.org/10.1007/978-3-662-44324-8_1531

The fluorescence emission of molecular species depends on their intrinsic physicochemical characteristics and on their interactions with the surrounding media. Therefore, fluorescence emission is extremely sensitive to changes of the environmental conditions in the fluorophore’s vicinity (e.g., pH, temperature, presence of fluorescence quenchers, solvent properties). Together, these factors define the period during which a fluorophore remains in the excited state, i.e., fluorescence lifetime, and the magnitude of each radiative and non-radiative de-excitation process component, through which the fluorophore decays to the ground state (the lowest energy level).

This dependency relation limits the use of fluorescence spectroscopy for quantitative purposes, but simultaneously it explains the potential of this technique to provide information about the system status using single or multiple fluorescence probes (fluorophores). The information about the molecular events occurring in the...
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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.REQUIMTE-LAQV, Chemistry DepartmentFCT-Universidade Nova de Lisboa, Campus da CaparicaCaparicaPortugal
  2. 2.REQUIMTE-CQFB, Chemistry Dept. Faculdade de Ciências e TecnologiaFCT-Universidade Nova de Lisboa, Campus da CaparicaCaparicaPortugal