Encyclopedia of Astrobiology

2015 Edition
| Editors: Muriel Gargaud, William M. Irvine, Ricardo Amils, Henderson James (Jim) CleavesII, Daniele L. Pinti, José Cernicharo Quintanilla, Daniel Rouan, Tilman Spohn, Stéphane Tirard, Michel Viso

Restriction Enzyme

Reference work entry
DOI: https://doi.org/10.1007/978-3-662-44185-5_1368



A restriction enzyme is an endonuclease that binds and cuts double-stranded  DNA at a specific nucleotide sequence. Some restriction enzymes cut both strands, generating blunt ends whereas others generate sticky ends. Restriction combined with DNA modification, which impede the cutting of the DNA, is a protection system against the introduction of foreign DNA sequences into prokaryotic systems. These enzymes have been instrumental in the development of  cloning and genetic engineering techniques.

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© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de ValènciaValènciaSpain