Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Absorbance Spectroscopy: Overview

  • Paul WormellEmail author
  • Alison Rodger
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_784-1



Most spectroscopic phenomena arise from the interaction of a molecule or other chemical species with one photon at a time. Multiphoton processes are less commonly encountered. The molecule may either absorb or scatter the photon; it may also emit a photon. The simplest form of spectroscopy is absorption, which measures how much electromagnetic radiation (e.g., light) of a given frequency is absorbed by a collection of molecules. If a molecule absorbs a photon of frequency ν, it increases its energy by
$$ \Delta E= h\nu = h c/\lambda $$
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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.School of Science and HealthWestern Sydney UniversityPenrithAustralia
  2. 2.Department of Molecular SciencesMacquarie UniversitySydneyAustralia

Section editors and affiliations

  • Alison Rodger
    • 1
  1. 1.Department of Molecular Sciences, Macquarie UniversityNWSAustralia