Absorption Spectroscopy to Probe Ligand Binding
When a ligand solution is added to a solution of biomacromolecules, if it does not bind to the macromolecules, then the UV–visible spectrum will simply be the sum of the macromolecule spectrum and the ligand spectrum. If the ligand does bind to the macromolecules, then the spectrum of the complex will be (at least slightly) different from the sum spectrum. One should note that the observed spectrum is probably a complicated mixture of the spectrum of bound and unbound ligands and free and complexed macromolecules.
- Schmechel DEV, Crothers DMB (1971) Kinetic and hydrodynamic studies of the complex of proflavine with poly A·poly U. Biolopymers 10:465–480Google Scholar