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Kinetics: Overview

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Synonyms

Chemical kinetics

Introduction

The root of Kinetics comes from kinesis (movement), and in general, it is a term applied to characterization of phenomena with respect to time. Every measurement is carried out as a function of time, and hence, there is potentially a kinetic element to every experiment. This section focuses on chemical kinetics which involves the investigation and interpretation of reaction rates. Here the concentrations of chemical species or states are measured with time, principally for the determination of reaction mechanism. Kinetics may also be used empirically to determine the concentrations of species. This is the main-stay of many clinical assays.

Kinetics is fundamental to many aspects of protein and nucleic acid chemistry, be it ligand binding, catalysis, or folding (Bagshaw 2017; Goodrich and Kugel 2006; Gutfreund 1995; Purich 2010). Clearly kinetic studies are central to the characterization of enzymes as catalysts, and many of the principles of...

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References

  • Aquila A, Hunter MS, Doak RB et al (2012) Time-Resolved Protein Nanocrystallography Using an X-Ray Free-Electron Laser. Opt Express 20:2706–2716

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bagshaw CR (2017) Biomolecular kinetics: a step-by-step guide. CRC Press, Boca Raton

    Book  Google Scholar 

  • Barman TE, Travers F (1985) The rapid-flow-quench method in the study of fast reactions in biochemistry: extension to subzero conditions. Meth Biochem Anal 31:1–59

    CAS  Google Scholar 

  • Bernasconi CF (1976) Relaxation kinetics. Academic, New York

    Google Scholar 

  • Chaiken I, Rose S, Karlsson R (1992) Analysis of macromolecular interactions using immobilized `ligands. Anal Biochem 201:197–210

    Article  CAS  PubMed  Google Scholar 

  • Cook PF, Cleland WW (2007) Enzyme kinetics and mechanism. Talyor & Francis, New York

    Google Scholar 

  • Cornish-Bowden A (1995) Fundamentals of enzyme kinetics, 2nd edn. Portland Press, London

    Google Scholar 

  • de Mol NJ (2010) Fischer MJE surface Plasmon resonance: methods and protocols. Methods Mol Biol 627:255

    Google Scholar 

  • Eccleston JF, Hutchinson JP, White HD (2001) Stopped-flow techniques. In: Harding SE, Chowdhry BZ (eds) Protein-ligand interactions. Oxford. Oxford University Press, New York, pp 201–237

    Google Scholar 

  • Engel PC (1981) Enzyme kinetics: the steady-state approach. Chapman and Hall, London

    Book  Google Scholar 

  • Gell C, Brockwell D, Smith A (2006) Handbook of single molecule fluorescence spectroscopy. Oxford University Press, Oxford

    Google Scholar 

  • Goodrich JA, Kugel JF (2006) Binding and kinetics for molecular biologists. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Goody RS (2014) How not to do kinetics: examples involving GTPases and guanine nucleotide exchange factors. FEBS J 281:593–600

    Article  CAS  PubMed  Google Scholar 

  • Gutfreund H (1995) Kinetics for the life sciences: receptors transmitters and catalysts. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Hiromi K (1979) Kinetics of fast enzyme reactions: theory and practice. Wiley, New York

    Google Scholar 

  • Johnson KA (ed) (2003) Kinetic analysis of macromolecules. Edited by Hames BD. Oxford university press, Oxford

    Google Scholar 

  • Kuzmic P (2009) DynaFit--a software package for enzymology. Methods Enzymol 467:247–280

    Article  CAS  PubMed  Google Scholar 

  • Makarov DE (2015) Single molecule science: physical principles and models. CRC Press, Boca Raton

    Book  Google Scholar 

  • Minton AP (2006) How can biochemical reactions within cells differ from those in test tubes? J Cell Sci 119:2863–2869

    Article  CAS  PubMed  Google Scholar 

  • Motulsky H, Christopoulos A (2004) Fitting models to biological data using linear and nonlinear regression: a practical guide to curve fitting. Oxford University Press, Oxford, UK

    Google Scholar 

  • Pudney CR, Hay S, Levy C, Pang J, Sutcliffe MJ, Leys D, Scrutton NS (2009) Evidence to support the hypothesis that promoting vibrations enhance the rate of an enzyme catalyzed H-tunneling reaction. J Am Chem Soc 131:17072–17073

    Article  CAS  PubMed  Google Scholar 

  • Purich DL (2010) Enzyme kinetics: Catalysis & Control: a reference of theory and best-practice methods. Academic Press, London

    Google Scholar 

  • Rule GS, Hitchens TK (2006) Fundamentals of protein NMR spectroscopy. Springer, Dordrecht

    Google Scholar 

  • Selvin PR, Ha T (eds) (2008) Single-molecule techniques: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Stagno JR, Liu Y, Bhandari YR et al (2017) Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography. Nature 7636:242–246

    Article  CAS  Google Scholar 

  • Taylor KB (2002) Enzyme kinetics and mechanisms. Kluwer Academic Publishers, Dordrecht

    Google Scholar 

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Correspondence to Clive R. Bagshaw .

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© 2018 European Biophysical Societies' Association (EBSA)

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Bagshaw, C.R. (2018). Kinetics: Overview. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_54-2

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  • DOI: https://doi.org/10.1007/978-3-642-35943-9_54-2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-35943-9

  • Online ISBN: 978-3-642-35943-9

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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Chapter history

  1. Latest

    Kinetics: Overview
    Published:
    07 August 2018

    DOI: https://doi.org/10.1007/978-3-642-35943-9_54-2

  2. Original

    Kinetics: Overview
    Published:
    11 June 2018

    DOI: https://doi.org/10.1007/978-3-642-35943-9_54-1