Skip to main content
SpringerLink
Log in
Book cover

The Prokaryotes pp 869–880Cite as

The Family Methylophilaceae

  • Reference work entry
  • First Online:

Abstract

Methylophilaceae, a family within the order Methylophilales, embraces the genera Methylophilus (type genus), Methylobacillus, Methylovorus, and Methylotenera. Betaproteobacterial obligate and restricted facultative methylotrophs capable of utilizing methanol or methylamine as a sole source of carbon and energy. Do not use methane (methylobacteria). Gram-negative rods, multiply by binary fission. Assimilate C1 compounds via the ribulose monophosphate (Quayle) cycle. Major fatty acids are C16:1ω7c and C16:0. However, obligate methylobacteria possess similar morphology and metabolic organization. Thus, the main criteria used to clarify obligate methylobacteria into separate genera and species are their genomic and phylogenetic characteristics. On the other hand, members of the family are defined by some chemotaxonomic and biochemical properties, such as specific phospholipids and enzymes which are used for the delineation of genera. Members of the family are mainly found in activated sludge, mud, river, lake and pond waters, and plants.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   699.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   849.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Anthony C, Williams P (2003) The structure and mechanism of methanol dehydrogenase. Biochim Biophys Acta 1647:18–23

    Article  CAS  PubMed  Google Scholar 

  • Chistoserdova L, Chen S-W, Lapidus A, Lidstrom ME (2003) Methylotrophy in Methylobacterium extorquens AM1 from a genomic point of view. J Bacteriol 185:2980–2987

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chistoserdova L, Lapidus A, Han C, Goodwin L, Saunders L, Brettin T, Tapia R, Gilna P, Lucas S, Richardson PM, Lidstrom ME (2007) Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. J Bacteriol 189:4020–4027

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chistoserdova L, Kalyuzhnaya MG, Lidstrom ML (2009) The expanding world of methylotrophic metabolism. Annu Rev Microbiol 63:477–499

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Chistoserdova L (2011) Modularity of methylotrophy, revisited. Environ Microbiol 13:2603–2622

    Article  CAS  PubMed  Google Scholar 

  • Doronina NV, Trotsenko YA (1986) Composition of the biomass of methanol-utilizing bacteria. Appl Biochem Microbiol 22:557–561

    CAS  Google Scholar 

  • Doronina NV, Trotsenko YA (1994) Methylophilus leisingerii sp. nov., a new species of restricted facultatively methylotrophic bacteria. Microbiol Engl Transl Mikrobiologiya 63:529–536

    CAS  Google Scholar 

  • Doronina NV, Kudinova LV, Trotsenko YA (2000) Methylovorus mays sp. nov.: a new species of aerobic, obligately methylotrophic bacteria associated with plants. Microbiol Engl Transl Mikrobiologiya 69:599–603

    CAS  Google Scholar 

  • Doronina NV, Ivanova EG, Trotsenko YA (2002) New evidence for the ability of methylobacteria and methanotrophs to synthesize auxins. Microbiol Engl Transl Mikrobiologiya 71:116–118

    CAS  Google Scholar 

  • Doronina NV, Trotsenko YA, Kolganova TV, Tourova TP, Salkinoja-Salonen MS (2004) Methylobacillus pratensis sp. nov., a novel non-pigmented, aerobic, obligately methylotrophic bacterium isolated from meadow grass. Int J Syst Evol Microbiol 54:1453–1457

    Article  CAS  PubMed  Google Scholar 

  • Doronina NV, Ivanova EG, Trotsenko YA (2005a) Phylogenetic position and emended description of the genus Methylovorus. Int J Syst Evol Microbiol 55:903–906

    Article  CAS  PubMed  Google Scholar 

  • Doronina N, Ivanova E, Trotsenko Y, Pshenichnikova A, Kalinina E, Shvets V (2005b) Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium. Syst Appl Microbiol 28:303–309

    Article  PubMed  Google Scholar 

  • Doronina NV, Fedorov DN, Trotsenko YA, Smolyanina SO, Berkovich YA (2009) Obligate methylotrophic bacteria stimulate morphogenesis and antifungal resistance of Chinese cabbage Brassica chinensis L. Biotechnol Russ 6:57–61

    Google Scholar 

  • Doronina NV, Kaparullina EN, Trotsenko YA (2011) Methylovorus menthalis, a novel species of aerobic obligate methylobacteria associated with plants. Microbiol Engl Transl Mikrobiologiya 80:700–706

    CAS  Google Scholar 

  • Doronina NV, Gogleva AA, Trotsenko YA (2012) Methylophilus glucosoxydans sp. nov., a restricted facultative methylotroph from rice rhizosphere. Int J Syst Evol Microbiol 62:196–201

    Article  CAS  PubMed  Google Scholar 

  • Fedorov DN, Doronina NV, Trotsenko YA (2011) Phytosymbiosis of aerobic methylobacteria: new facts and views. Microbiol Engl Transl Mikrobiologiya 80:435–446

    CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Galbally IE, Kirstine W (2002) The production of methanol by flowering plants and the global cycle of methanol. J Atmosph Chem 43:195–229

    Article  CAS  Google Scholar 

  • Gaelli R, Leisinger T (1985) Specialized bacterial strains for the removal of dichloromethane from industrial waste. Conserv Recycl 8:91–100

    Article  CAS  Google Scholar 

  • Garrity GM, Bell JA, Lilburn T (2005) Order III. Methylophilales ord. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2, 2nd edn, (The Proteobacteria), part C (the alpha-, beta-, delta-, and Epsilonproteobacteria). Springer, New York, p 770

    Google Scholar 

  • Giovannoni SJ, Hayakawa DH, Tripp HJ, Stingl U, Givan SA, Cho J-C, Oh H-M, Kitner JB, Vergin KL, Rappé MS (2008) The small genome of an abundant coastal ocean methylotroph. Environ Microbiol 10:1771–1782

    Article  CAS  PubMed  Google Scholar 

  • Gogleva AA, Kaparullina EN, Doronina NV, Trotsenko YA (2010) Methylophilus flavus sp. nov., and Methylophilus luteus sp. nov., aerobic, methylotrophic bacteria associated with plants. Int J Syst Evol Microbiol 60:2623–2628

    Article  CAS  PubMed  Google Scholar 

  • Gogleva AA, Kaparullina EN, Doronina NV, Trotsenko YA (2011) Methylobacillus arboreus sp. nov., and Methylobacillus gramineus sp. nov., novel non-pigmented obligately methylotrophic bacteria associated with plants. Syst Appl Microbiol 34:477–481

    Article  CAS  PubMed  Google Scholar 

  • Govorukhina NI, Kletsova LV, Tsygankov YD, Trotsenko YA, Netrusov AI (1987) Characteristics of a new obligate methylotroph. Microbiol Engl Transl Mikrobiologiya 56:849–854

    CAS  Google Scholar 

  • Govorukhina NI, Trotsenko YA (1991) Methylovorus, a new genus of restricted facultatively methylotrophic bacteria. Int J Syst Bacteriol 41:158–162

    Article  CAS  Google Scholar 

  • Harder W, Attwood M, Quayele JR (1973) Methanol assimilation by Hyphomicrobium spp. J Gen Microbiol 78:155–163

    Article  CAS  Google Scholar 

  • Hendrickson EL, Beck DAC, Wang T, Lidstrom ME, Hackett M, Chistoserdova L (2010) Expressed genome of Methylobacillus flagellatus as defined through comprehensive proteomics and new insights into methylotrophy. J Bacteriol 192:4859–4867

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ivanova EG, Doronina NV, Shepelyakovskaya AO, Laman AG, Brovko FA, Trotsenko YA (2000) Facultative and obligate aerobic methylobacteria synthesize cytokinins. Microbiol Engl Transl Mikrobiologiya 69:646–651

    CAS  Google Scholar 

  • Jenkins O, Byrom D, Jones D (1987) Methylophilus: a new genus of methanol-utilizing bacteria. Int J Syst Bacteriol 37:446–448

    Article  Google Scholar 

  • Kalyaeva MA, Zakharchenko NS, Doronina NV, Rukavtsova EB, Ivanova EG, Alekseeva VV, Trotsenko YA, Bur’yanov YI (2001) Plant growth and morphogenesis in vitro is promoted by associative methylotrophic bacteria. Rus J Plant Physiol Engl Transl Fiziol Rast 48:514–517

    Article  CAS  Google Scholar 

  • Kalyuzhnaya MG, Bowerman S, Lara JC, Lidstrom ME, Chistoserdova L (2006) Methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium within the family Methylophilaceae. Int J Syst Evol Microbiol 56:2819–2823

    Article  CAS  PubMed  Google Scholar 

  • Kalyuzhnaya MG, Beck DAC, Vorob’ev A, Smalley N, Kunkel DD, Lidstrom ME, Chistoserdova L (2012) Novel methylotrophic isolates from lake sediment, description of Methylotenera versatilis sp. nov. and emended description of the genus Methylotenera. Int J Syst Evol Microbiol 62:106–111

    Article  CAS  PubMed  Google Scholar 

  • Kane SR, Chakicherla AY, Chain PSG, Schmidt R, Shin MW, Legler TC, Scow KM, Larimer FW, Lucas SM, Richardson PM, Hristova KR (2007) Whole-genome analysis of the methyl tert-butyl ether-degrading beta-proteobacterium Methylibium petroleiphilum PM1. J Bacteriol 189:1931–1945

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lapidus A, Clum A, Labutti K, Kaluzhnaya MG, Lim S, Beck DAC, Glavina Del Rio T, Nolan M, Mavromatis K, Huntemann M, Lucas S, Lidstrom ME, Ivanova N, Chistoserdova L (2011) Genomes of three methylotrophs from a single niche reveal the genetic and metabolic divergence of the Methylophilaceae. J Bacteriol 193:3757–3764

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Large PJ, Bamforth WC (1988) Methylotrophy and biotechnology. Wiley, New York, p 303

    Google Scholar 

  • MacLennan DG, Ousby JC, Owen TR, Steer DC (1974) Microbiological production of protein. UK patent no. GB1370892

    Google Scholar 

  • Madhaiyan M, Poonguzhali S, Kwon S-W, Sa T-M (2009) Methylophilus rhizosphaerae sp. nov., a restricted facultative methylotroph isolated from rice rhizosphere soil. Int J Syst Evol Microbiol 59:2904–2908

    Article  CAS  PubMed  Google Scholar 

  • Nemecek-Marshall M, MacDonald RC, Franzen JJ, Wojciechowski CL, Fall R (1995) Methanol emission from leaves: enzymatic detection of gas-phase methanol and relation of methanol fluxes to stomatal conductance and leaf development. Plant Physiol 108:1359–1368

    CAS  PubMed Central  PubMed  Google Scholar 

  • Seo SA, Kim YM (1993) Isolation and characterization of a restricted facultatively methylotrophic bacterium Methylovorus sp. strain SS1. Kor J Microbiol 31:179–183

    CAS  Google Scholar 

  • Sokolov AP, Luchin SV, Trotsenko YA (1980) Purification and properties of glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Methylobaillus sp. Biochemistry (Moscow) 45:1371–1378

    CAS  Google Scholar 

  • Sokolov AP, Trotsenko YA (1987) Purification and properties of NADP-dependent glutamate dehydrogenase from the obligate methylotrophic bacterium Methylophilus rnethanolovorus. Biochemistry (Moscow) 52:1417–1421

    CAS  Google Scholar 

  • Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526

    CAS  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Trotsenko YA, Doronina NV, Khmelenina VN (2005) Biotechnological potential of aerobic methylotrophic bacteria: current state and future prospects. Appl Biochem Microbiol 41:433–441

    Article  CAS  Google Scholar 

  • Trotsenko YA, Ivanova EG, Doronina NV (2001) Aerobic methylotrophic bacteria as phytosymbionts. Microbiol Engl Transl Mikrobiologiya 70:623–632

    CAS  Google Scholar 

  • Urakami T, Komagata K (1986) Emendation of Methylobacillus Yordy and Weaver 1977, a genus of methanol-utilizing bacteria. Int J Syst Bacteriol 36:502–511

    Article  Google Scholar 

  • Ward N, Larsen O, Sakwa J, Bruseth L, Khouri H, Durkin AS, Dimitrov G, Jiang L, Scanlan D, Kang KH, Lewis M, Nelson KE, Methe B, Wu M, Heidelberg JF, Paulsen IT, Fouts D, Ravel J, Tettelin H, Ren Q, Read T, DeBoy RT, Seshadri R, Salzberg SL, Jensen HB, Birkeland NK, Nelson WC, Dodson RJ, Grindhaug SH, Holt I, Eidhammer I, Jonasen I, Vanaken S, Utterback T, Feldblyum TV, Fraser CM, Lillehaug JR, Eisen JA (2004) Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (bath). PLoS Biol 2:E303

    Article  PubMed Central  PubMed  Google Scholar 

  • Whittenbury R, Phillips KC, Wilkinson JF (1970) Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol 61:205–218

    Article  CAS  PubMed  Google Scholar 

  • Yordy JR, Weawer TL (1977) Methylobacillus: a new genus of obligately methylotrophic bacteria. Int J Syst Bact 27:247–255

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    Nina Doronina, Elena Kaparullina & Yuri Trotsenko

Authors
  1. Nina Doronina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elena Kaparullina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuri Trotsenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nina Doronina .

Editor information

Editors and Affiliations

  1. Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv, Israel

    Eugene Rosenberg

  2. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA

    Edward F. DeLong

  3. Center for Microbial Oceanography: Research and Education, University of Hawaii, Manoa, Honolulu, HI, USA

    Edward F. DeLong

  4. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA

    Stephen Lory

  5. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    Erko Stackebrandt

  6. Laboratory of Microbiology, Institute of Biology, Center for Health Sciences, Federal University of Rio de Janeiro (UFRJ), Ilha do Fundão, Rio de Janeiro, Brazil

    Fabiano Thompson

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer-Verlag Berlin Heidelberg

About this entry

Cite this entry

Doronina, N., Kaparullina, E., Trotsenko, Y. (2014). The Family Methylophilaceae. In: Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., Thompson, F. (eds) The Prokaryotes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-30197-1_243

Download citation

Publish with us

Policies and ethics

Search

Navigation