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Measurements of Reactive Oxygen Species in Cardiovascular Studies

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Systems Biology of Free Radicals and Antioxidants

Abstract

In the cardiovascular system, reactive oxygen species (ROS) are produced mainly by NADPH oxidases, uncoupled nitric oxide synthase, and mitochondria. Superoxide and hydrogen peroxide are two major players in ROS-mediated cell signaling and oxidative stress, which contribute to the development of a number of pathological conditions including heart failure, ischemia, arteriosclerosis, and hypertension. Meanwhile, studies of physiological and pathological roles of ROS are significantly limited by the lack of site-specific and selective ROS detection. Detection of ROS at the specific subcellular sites of their generation is important for better understanding of their sources and mechanisms regulating ROS production and to develop treatment preventing uncontrolled redox signaling events leading to oxidative stress and cell malfunctions. In this chapter we review existing methods for ROS detection with emphasis on reliable methods for site-specific and selective ROS measurement.

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Authors and Affiliations

  1. Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA

    Sergey I. Dikalov & Rafal R. Nazarewicz

Authors
  1. Sergey I. Dikalov
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  2. Rafal R. Nazarewicz
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Correspondence to Sergey I. Dikalov .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Therapeutics, University of British Colombia, Vancouver, British Columbia, Canada

    Ismail Laher

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Dikalov, S.I., Nazarewicz, R.R. (2014). Measurements of Reactive Oxygen Species in Cardiovascular Studies. In: Laher, I. (eds) Systems Biology of Free Radicals and Antioxidants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-30018-9_45

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