Encyclopedia of Cancer

Living Edition
| Editors: Manfred Schwab

Replication Licensing System

Living reference work entry

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DOI: https://doi.org/10.1007/978-3-642-27841-9_5037-4

Definition

The “Replication Licensing System” is critical for the maintenance of genome stability by ensuring that no section of chromosomal genomic DNA is replicated more than once within a single cell division cycle (Arias and Walter 2007; Blow and Dutta 2005; Blow and Gillespie 2008). In late M and G1 phase, but not at other stages of the cell cycle, replication origins are licensed for a single initiation event in the upcoming S phase by encircling DNA with double hexamers of the minichromosome maintenance proteins, MCM2–7 (Arias and Walter 2007; Blow and Dutta 2005; Blow and Gillespie 2008; Deegan and Diffley 2016; Riera et al. 2017). During S phase of the cell cycle, DNA-bound MCM2–7 are activated to form the primary replicative helicase that unwinds the DNA ahead of each replication fork (Deegan and Diffley 2016; Riera et al. 2017). When replication forks terminate, MCM2–7 are removed from the DNA, thus ensuring that no section of the genome is rereplicated in a single cell...

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References

  1. Arias EE, Walter JC (2007) Strength in numbers: preventing rereplication via multiple mechanisms in eukaryotic cells. Genes Dev 21:497–518CrossRefGoogle Scholar
  2. Blow JJ, Dutta A (2005) Preventing re-replication of chromosomal DNA. Nat Rev Mol Cell Biol 6:476–486CrossRefGoogle Scholar
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  4. Deegan TD, Diffley JF (2016) MCM: one ring to rule them all. Curr Opin Struct Biol 37:145–151CrossRefGoogle Scholar
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  7. Riera A, Barbon M, Noguchi Y, Reuter LM, Schneider S, Speck C (2017) From structure to mechanism-understanding initiation of DNA replication. Genes Dev 31:1037–1088CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centre for Gene Regulation & Expression, Wellcome Trust BiocentreSchool of Life Sciences, University of DundeeDundeeUK