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Encyclopedia of Biophysics pp 1637–1646Cite as

Multidimensional NMR Spectroscopy

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Synonyms

Fourier transform NMR spectroscopy

Definition

In multidimensional nuclear magnetic resonance (NMR) spectroscopy nuclear spins, polarized in a static magnetic field, are brought in a non-equilibrium state during a preparation period and undergo one or more cycles of frequency labeling and mixing before they are detected. Signals in most multidimensional NMR spectra have coordinates that are the chemical shifts of nuclear spins which have been correlated during the different mixing periods, either by through-bond or by through-space magnetic interactions between them. Multidimensional NMR makes it possible to determine the chemical and spatial structures of molecules.

Introduction: From 1D to 2D Fourier Transform NMR Spectroscopy

About 50 years ago, transitions between nuclear spin states were monitored by slowly sweeping the magnetic field B through the respective resonance conditions of the spins, while keeping the frequency of the photons used for irradiation constant. This...

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Author information

Authors and Affiliations

  1. Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    Frans A. A. Mulder & Ruud M. Scheek

  2. Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, Langelandsgade 140, DK-8000, Aarhus, Denmark

    Frans A. A. Mulder

Authors
  1. Frans A. A. Mulder
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  2. Ruud M. Scheek
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Corresponding author

Correspondence to Frans A. A. Mulder .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Leicester, Leicester, UK

    Gordon C. K. Roberts

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© 2013 European Biophysical Societies' Association (EBSA)

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Mulder, F.A.A., Scheek, R.M. (2013). Multidimensional NMR Spectroscopy. In: Roberts, G.C.K. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16712-6_307

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