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In Vitro Methods in Renal Research

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Pediatric Nephrology

Abstract

Significant advances in the understanding of normal and disease-associated renal cell function, epithelial cell biology, and morphogenesis have been made in recent years by the application of in vitro techniques. The researcher today is presented with a wide choice of in vitro models, and the aim of this chapter is to provide not only an overview of techniques available but also sufficient information to allow insight into the advantages and limitations of each system. This provides the experimental pediatric nephrologist with an appreciation of the range of renal in vitro methods currently available and allows selection of the most appropriate in vitro system to adequately answer the questions posed. For a more complete methodologic review of isolation and culture techniques, the reader is referred to standard tissue culture texts (1–3).

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Authors
  1. Patricia D. Wilson
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Editors and Affiliations

  1. Medical College of Wisconsin, Wisconsin, USA

    Ellis Avner (Director, Children's Research Institute, CHHS, Associate Dean for Research, Professor of Pediatrics and Physiology) (Director, Children's Research Institute, CHHS, Associate Dean for Research, Professor of Pediatrics and Physiology)

  2. Harvard Medical School, Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts, 02115, USA

    William Harmon (Professor of Pediatrics, Director, Pediatric Nephrology) (Professor of Pediatrics, Director, Pediatric Nephrology)

  3. Service de Néphrologie Pédiatrique Centre de référence des Maladies Rénales Héréditaires de l'Enfant et de l'Adulte (MARHEA), Hôpital Necker‐Enfants Malades, 149, rue de Sèvres, 75743, Paris Cedex 15, France

    Patrick Niaudet

  4. Department of Pediatrics Wakayama Medical University, Wakayama Medical University Hospital, 641–8510811–1, Kimiidera, Wakayama City, Japan

    Norishige Yoshikawa (Professor and Chair Pediatrician‐in‐Chief) (Professor and Chair Pediatrician‐in‐Chief)

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© 2009 Springer-Verlag Berlin Heidelberg

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Wilson, P.D. (2009). In Vitro Methods in Renal Research. In: Avner, E., Harmon, W., Niaudet, P., Yoshikawa, N. (eds) Pediatric Nephrology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-76341-3_15

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  • DOI: https://doi.org/10.1007/978-3-540-76341-3_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-76327-7

  • Online ISBN: 978-3-540-76341-3

  • eBook Packages: MedicineReference Module Medicine

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