Antidromic Microstimulation Mapping
Antidromic microstimulation is a technique that can be used to map the locations of the cell bodies of origin of a nervous system pathway. An electrical stimulus is applied through a microelectrode that is inserted into a nervous system region of interest. The stimulus intensity is kept minimal to prevent stimulus spread. A series of microelectrode tracks are made transversely across a region suspected to contain the cells of origin of the pathway terminating near the stimulating electrode. Recordings are made through this electrode so that antidromically activated neurons can be identified. The stimulating and recording sites are reconstructed after the experiment, often with the assistance of electrolytic lesions or other types of marks made by passing current through the electrodes.