Synonyms
aIF6 in Archae; Tif6p (in yeast)
Historical Background
- (a)
Isolation and initial characterization of eIF6 – Eukaryotic initiation factor 6 (eIF6), a monomeric protein of about 26.5 kDa, was originally isolated from the postribosomal supernatant of both wheat germ (Russel and Spremulli 1979) and mammalian cell extracts (Valenzuela et al. 1982) based on an in vitro assay that measured the ability of the protein to bind specifically to the 60S ribosomal subunit and to prevent the association of the 60S subunit to the 40S ribosomal subunit to form the 80S ribosomes. Because, the assembly of the 80S initiation complex during initiation of protein synthesis requires a cellular pool of free 40S and 60S ribosomal subunits, eIF6 was thought to play a direct role in the provision of free ribosomal subunits required for initiation of protein synthesis. The protein was therefore classified as a eukaryotic translation initiation factor (eIF), although its function in translation was...
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Basu U, Si K, Warner JR, Maitra U. The Sacchamomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol Cell Biol. 2001;21:1453–62.
Basu U, Si K, Deng H, Maitra U. Phosphorylation of mammalian translation initiation factor 6 and its Saccharomyces cerevisiae homologue Tif6p: evidence that phosphorylation of Tif6p regulates its nucleocytoplasmic distribution and is required for yeast cell growth. Mol Cell Biol. 2003;23:6187–99.
Becam AM, Nasr F, Racki WJ, Zagulski M, Herbert CJ. Ria1p (ynl163c), a protein similar to elongation factor 2, is involved in the biogenesis of 60S subunit of the ribosome in Saccharomyces cerevisiae. Mol Gen Genomics. 2001;266:454–62.
Biswas A, Mukherjee S, Das S, Shields D, Chow CW, Maitra U. Opposing action of casein kinase 1 and calcineurin in nucleo-cytoplasmic shuttling of mammalian translation initiation factor eIF6. J Biol Chem. 2011;286:3129–38.
Ceci M, Gaviraghi C, Gorrini C, Sala LA, Offenhauser N, Marchisio PC, Biffo S. Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly. Nature. 2003;426:579–84.
Finch AJ, Hilcenko C, Basse N, et al. Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond Syndrome. Genes Dev. 2011;25:917–29.
Gartmann M, Blau M, Armache J-P, Mielke T, Topf M, Beckmann R. Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. J Biol Chem. 2010;285:14848–51.
Kressler D, Hurt E, Bassler J. Driving ribosome assembly. Biochem Biophys Acta. 2010;1803:673–83.
Menne TF, Goyenechea B, Sanchez-Puig N, Wong CC, Tonkin LM, Ancliff PJ, Brost RL, Costanzo M, Boone C, Warren AJ. The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet. 2007;39:486–95.
Miluzio A, Beugnet A, Volta V, Biffo S. Eukaryotic initiation factor 6 mediates a continuum between 60S ribosome biogenesis and translation. EMBO Rep. 2009;10:459–65.
Panse VG, Johnson AW. Maturation of eukaryotic ribosomes: acquisition of functionality. Trends Biochem Sci. 2010;35:260–6.
Ray P, Basu U, Ray A, Majumdar R, Deng H, Maitra U. The Saccharomyces cerevisiae 60S ribosome biogenesis factor Tif6p is regulated by Hrr25p-mediated phosphorylation. J Biol Chem. 2008;283:9681–91.
Russel DW, Spremulli LL. Purification and characterization of a ribosome dissociation factor (eukaryotic initiation factor 6) from wheat germ. J Biol Chem. 1979;254:8796–800.
Sanvito F, Piatti S, Villa A, Bossi M, Lucchini G, Marchisio PC, Biffo S. The beta 4 integrin interactor p27 (BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly. J Cell Biol. 1999;144:823–37.
Senger B, Lafontaine DLJ, Graindorge J-S, Gadal O, Camasses A, Sanni A, Garnier J-M, Breitenbach M, Hurt E, Fasiolo F. The nucle(ol)ar Tif6p and Efl1p are required for a late cytolasmic step of ribosome synthesis. Mol Cell. 2001;8:1363–73.
Si K, Maitra U. The Saccharomyces cerevisiae homologue of mammalian translation initiation factor 6 does not function as a translation initiation factor. Mol Cell Biol. 1999;19:1416–26.
Si K, Chaudhuri J, Chevesich J, Maitra U. Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6. Proc Natl Acad Sci USA. 1997;94:14285–90.
Valenzuela DM, Chaudhuri A, Maitra U. Eukaryotic ribosomal subunit anti-association activity of calf liver is contained in a single polypeptide chain protein of Mr=25,500 (eukaryotic initiation factor 6). J Biol Chem. 1982;257:7712–9.
Venema J, Tollervey D. Ribosome synthesis in Saccharomyces cerevisiae. Annu Rev Genet. 1999;33:261–311.
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Biswas, A., Choudhuri, A., Maitra, U. (2018). TIF6 (eIF6). In: Choi, S. (eds) Encyclopedia of Signaling Molecules. Springer, Cham. https://doi.org/10.1007/978-3-319-67199-4_615
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DOI: https://doi.org/10.1007/978-3-319-67199-4_615
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