Net1 (Neuroepithelial Cell Transforming Gene 1 Protein)
Rho family small GTPases control multiple cell functions, including organization of the actin cytoskeletal, cell motility and invasion, and cell cycle progression. They act as molecular switches, cycling between their active GTP-bound and inactive GDP-bound states. When bound to GTP, Rho GTPases initiate intracellular signaling by binding to downstream proteins known as effectors (Jaffe and Hall 2005). The activation state of Rho GTPases is controlled by three families of proteins, known as GDP exchange factors (Rho GEFs), GTPase activating proteins (Rho GAPs), and guanine nucleotide dissociation inhibitors (Rho GDIs). Rho GEFs activate Rho proteins by stimulating the release of GDP, thereby allowing the binding of GTP (Rossman et al. 2005; Meller et al. 2005). Rho GAPs accelerate the intrinsic GTPase activity of Rho proteins to hydrolyze GTP to GDP (Tcherkezian and Lamarche-Vane 2007). Rho GDIs sequester inactive, GDP-bound Rho GTPases...
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