Encyclopedia of Signaling Molecules

2018 Edition
| Editors: Sangdun Choi

Myosin III

  • Shigeru KomabaEmail author
Reference work entry
DOI: https://doi.org/10.1007/978-3-319-67199-4_541


Historical Background

Class III myosins are one of many classes of  myosin motor proteins. The first class III myosin, ninaC (neither inactivation nor afterpotential C), was identified in Drosophila as a gene responsible for abnormal phototransduction (Montell and Rubin 1988). This novel class of myosin is unique in having an N-terminal putative kinase domain joined to a myosin motor domain. The second class III myosin was isolated from the horseshoe crab Limulus polyphemus as a phosphoprotein regulated by a circadian clock; it is exclusively expressed in photoreceptors (Battelle et al. 1998). Subsequently, human class III myosin, MYO3A, was isolated by degenerated polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR from retina and a retinal pigment epithelial cell line (Dose and Burnside 2000). A shorter class III isoform, MYO3B, encoded by a different gene from MYO3A, was also identified (Dose and...

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  1. Battelle BA, Andrews AW, Calman BG, Sellers JR, Greenberg RM, Smith WC. A myosin III from Limulus eyes is a clock-regulated phosphoprotein. J Neurosci. 1998;18(12):4548–59.CrossRefPubMedGoogle Scholar
  2. Cronin MA, Diao F, Tsunoda S. Light-dependent subcellular translocation of Gqalpha in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINAC. J Cell Sci. 2004;117(Pt 20):4797–806.CrossRefPubMedGoogle Scholar
  3. Dose AC, Burnside B. Cloning and chromosomal localization of a human class III myosin. Genomics. 2000;67(3):333–42.CrossRefPubMedGoogle Scholar
  4. Dose AC, Burnside B. A class III myosin expressed in the retina is a potential candidate for Bardet-Biedl syndrome. Genomics. 2002;79(5):621–4.CrossRefPubMedGoogle Scholar
  5. Dose AC, Hillman DW, Wong C, Sohlberg L, Lin-Jones J, Burnside B. Myo3A, one of two class III myosin genes expressed in vertebrate retina, is localized to the calycal processes of rod and cone photoreceptors and is expressed in the sacculus. Mol Biol Cell. 2003;14(3):1058–73.PubMedPubMedCentralCrossRefGoogle Scholar
  6. Dose AC, Ananthanarayanan S, Moore JE, Corsa AC, Burnside B, Yengo CM. The kinase domain alters the kinetic properties of the myosin IIIA motor. Biochemistry. 2008;47(8):2485–96.CrossRefPubMedGoogle Scholar
  7. Erickson FL, Corsa AC, Dose AC, Burnside B. Localization of a class III myosin to filopodia tips in transfected HeLa cells requires an actin-binding site in its tail domain. Mol Biol Cell. 2003;14(10):4173–80.PubMedPubMedCentralCrossRefGoogle Scholar
  8. Hicks JL, Liu X, Williams DS. Role of the ninaC proteins in photoreceptor cell structure: ultrastructure of ninaC deletion mutants and binding to actin filaments. Cell Motil Cytoskeleton. 1996;35(4):367–79.CrossRefPubMedGoogle Scholar
  9. Kambara T, Komaba S, Ikebe M. Human myosin III is a motor having an extremely high affinity for actin. J Biol Chem. 2006;281(49):37291–301.CrossRefPubMedGoogle Scholar
  10. Kempler K, Toth J, Yamashita R, Mapel G, Robinson K, Cardasis H, et al. Loop 2 of limulus myosin III is phosphorylated by protein kinase A and autophosphorylation. Biochemistry. 2007;46(14):4280–93.PubMedPubMedCentralCrossRefGoogle Scholar
  11. Komaba S, Inoue A, Maruta S, Hosoya H, Ikebe M. Determination of human myosin III as a motor protein having a protein kinase activity. J Biol Chem. 2003;278(24):21352–60.CrossRefPubMedGoogle Scholar
  12. Komaba S, Watanabe S, Umeki N, Sato O, Ikebe M. Effect of phosphorylation in the motor domain of human myosin IIIA on its ATP hydrolysis cycle. Biochemistry. 2009;49(17):3695–702.CrossRefGoogle Scholar
  13. Lee SJ, Montell C. Light-dependent translocation of visual arrestin regulated by the NINAC myosin III. Neuron. 2004;43(1):95–103.CrossRefPubMedGoogle Scholar
  14. Li HS, Porter JA, Montell C. Requirement for the NINAC kinase/myosin for stable termination of the visual cascade. J Neurosci. 1998;18(23):9601–6.CrossRefPubMedGoogle Scholar
  15. Lin-Jones J, Parker E, Wu M, Dose A, Burnside B. Myosin 3A transgene expression produces abnormal actin filament bundles in transgenic Xenopus laevis rod photoreceptors. J Cell Sci. 2004;117(Pt 24):5825–34.CrossRefPubMedGoogle Scholar
  16. Meyer NE, Joel-Almagor T, Frechter S, Minke B, Huber A. Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade. J Cell Sci. 2006;119(Pt 12):2592–603.PubMedPubMedCentralCrossRefGoogle Scholar
  17. Montell C, Rubin GM. The Drosophila ninaC locus encodes two photoreceptor cell specific proteins with domains homologous to protein kinases and the myosin heavy chain head. Cell. 1988;52(5):757–72.CrossRefPubMedGoogle Scholar
  18. Ng KP, Kambara T, Matsuura M, Burke M, Ikebe M. Identification of myosin III as a protein kinase. Biochemistry. 1996;35(29):9392–9.CrossRefPubMedGoogle Scholar
  19. Porter JA, Montell C. Distinct roles of the Drosophila ninaC kinase and myosin domains revealed by systematic mutagenesis. J Cell Biol. 1993;122(3):601–12.CrossRefPubMedGoogle Scholar
  20. Porter JA, Hicks JL, Williams DS, Montell C. Differential localizations of and requirements for the two Drosophila ninaC kinase/myosins in photoreceptor cells. J Cell Biol. 1992;116(3):683–93.CrossRefPubMedGoogle Scholar
  21. Porter JA, Yu M, Doberstein SK, Pollard TD, Montell C. Dependence of calmodulin localization in the retina on the NINAC unconventional myosin. Science. 1993;262(5136):1038–42.CrossRefPubMedGoogle Scholar
  22. Salles FT, Merritt Jr RC, Manor U, Dougherty GW, Sousa AD, Moore JE, et al. Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments. Nat Cell Biol. 2009;11(4):443–50.PubMedPubMedCentralCrossRefGoogle Scholar
  23. Satoh AK, Ready DF. Arrestin1 mediates light-dependent rhodopsin endocytosis and cell survival. Curr Biol. 2005;15(19):1722–33.CrossRefPubMedGoogle Scholar
  24. Schneider ME, Dose AC, Salles FT, Chang W, Erickson FL, Burnside B, et al. A new compartment at stereocilia tips defined by spatial and temporal patterns of myosin IIIa expression. J Neurosci. 2006;26(40):10243–52.CrossRefPubMedGoogle Scholar
  25. Walsh T, Walsh V, Vreugde S, Hertzano R, Shahin H, Haika S, et al. From flies’ eyes to our ears: mutations in a human class III myosin cause progressive nonsyndromic hearing loss DFNB30. Proc Natl Acad Sci U S A. 2002;99(11):7518–23.PubMedPubMedCentralCrossRefGoogle Scholar
  26. Wes PD, XZ X, Li HS, Chien F, Doberstein SK, Montell C. Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD. Nat Neurosci. 1999;2(5):447–53.CrossRefPubMedGoogle Scholar

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© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Boston Biomedical Research InstituteWatertownUSA