Encyclopedia of Signaling Molecules

2018 Edition
| Editors: Sangdun Choi

Myosin III

Reference work entry
DOI: https://doi.org/10.1007/978-3-319-67199-4_541

Synonyms

Historical Background

Class III myosins are one of many classes of  myosin motor proteins. The first class III myosin, ninaC (neither inactivation nor afterpotential C), was identified in Drosophila as a gene responsible for abnormal phototransduction (Montell and Rubin 1988). This novel class of myosin is unique in having an N-terminal putative kinase domain joined to a myosin motor domain. The second class III myosin was isolated from the horseshoe crab Limulus polyphemus as a phosphoprotein regulated by a circadian clock; it is exclusively expressed in photoreceptors (Battelle et al. 1998). Subsequently, human class III myosin, MYO3A, was isolated by degenerated polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR from retina and a retinal pigment epithelial cell line (Dose and Burnside 2000). A shorter class III isoform, MYO3B, encoded by a different gene from MYO3A, was also identified (Dose and Burnside 2002).

Structure

Drosophila has a single myosin III gene that expresses two alternative isoforms, p174 (long isoform) and p132 (short isoform) (Fig. 1). The long isoform has two IQ motifs, whereas the short isoform has a single IQ motif. The myosin III gene in horseshoe crab encodes a single isoform with a single IQ motif and a short tail. Vertebrates possess two genes that code for two distinct isoforms, myosin IIIA and myosin IIIB (Fig. 1). Similar to other myosin classes, myosin III consists of a motor domain, a neck domain, and a tail domain in addition to an N-terminal kinase domain. There is a large diversity in amino acid sequence of the motor domain among class III myosins, e.g., there is only 30% homology between myosin III from human and Drosophila. The neck region binds 1–4 light chains such as calmodulin. Vertebrate myosin IIIA has an additional 1–5 calmodulin-binding site in the tail. There are two conserved sequences in the tail domain of vertebrate myosin III, class III tail homology domain I (3THDI), and class III tail homology domain II (3THDII) (Dose et al. 2003). 3THDI is present in both myosin IIIA and  myosin IIIB. 3THDII is located at the C-terminus of myosin IIIA and is an ATP-independent actin-binding domain, which is found in  myosin light chain kinase (Erickson et al. 2003). Heavy meromyosin-like human myosin III consisting of a kinase domain, a motor domain, a neck region, and a partial tail domain is monomeric (Komaba et al. 2003), which is consistent with the observation that human myosin III does not have a long coiled-coil region with which to form dimers.
Myosin III, Fig. 1

Diagram of invertebrate myosin III, vertebrate myosin IIIA, and vertebrate myosin IIIB. Class III myosin consists of a kinase domain, a motor domain, a neck domain with IQ motifs, and a tail domain

Biochemical and Biophysical Properties

Given its sequence similarity to other myosins, it was predicted that class III myosins function as molecular motors, enzymes characterized by the ability to interact with actin to produce mechanical force by hydrolyzing ATP. Hicks et al. demonstrated that Drosophila myosin III in the soluble fraction of a retinal homogenate binds to actin in an ATP-sensitive manner (Hicks et al. 1996), although to date there has been no report demonstrating that purified Drosophila myosin III has ATPase activity. The sequence of the phosphate-binding loop or P-loop, known as a nucleotide-binding motif, consists of GESGAGKT in many myosins; however, the sequence of Drosophila myosin III is GESYSGKS. Indeed, myosin III from horseshoe crab binds actin, but lacks ATPase activity (Kempler et al. 2007). Amino acids which form a salt bridge, E459-R238 in the case of Dictiostelium myosin II, are essential for ATP hydrolysis. Horseshoe crab myosin III has H487 at the equivalent position for R238. The first experimental evidence to demonstrate that myosin III is an actual motor protein was reported in 2003 (Komaba et al. 2003). The authors showed that human myosin IIIA missing the tail domain has an ATPase activity with Vmax of 0.34 s−1 and is capable of gliding actin filament at 0.11 μm/s, which is relatively slower compared to many other myosins. Kinetic analysis demonstrated that the dephosphorylated motor domain of human myosin IIIA spends the majority of its time during the ATP hydrolysis cycle attached to actin suggesting that myosin III moves along actin cables without dissociating (Kambara et al. 2006).

Phosphorylation/Regulation

Kinase activity in a class III myosin was originally observed in expressed myosin III from Drosophila, where the kinase domain phosphorylates various substrates including itself (Ng et al. 1996); however, the biochemical or physiological role of autophosphorylation of Drosophila myosin III remains unclear. Drosophila myosin III is phosphorylated in the p174 tail domain by protein kinase C and this phosphorylation is required for normal phototransduction, although the effect of phosphorylation on the biochemical properties of myosin III has not been shown (Li et al. 1998).

Horseshoe crab myosin III is also autophosphorylated and phosphorylated by protein kinase A (PKA) in loop 2, which is at the interface of actin binding (Kempler et al. 2007), suggesting that the interaction between myosin III and actin is modulated by cAMP-mediated phosphorylation and/or autophosphorylation.

Human myosin IIIA also undergoes autophosphorylation in the motor domain, which significantly reduces its affinity for actin (Kambara et al. 2006) resulting in a decrease in the duty ratio (Komaba et al. 2009). Deletion of the kinase domain affects both the biochemical and cell biological properties of myosin IIIA. A kinase domain-deletion mutant has a twofold higher Vmax and fivefold higher affinity for actin, compared to that of myosin IIIA with the kinase domain (Dose et al. 2008). Erickson et al. reported that striped bass myosin IIIA localizes to the cytoplasm and to the tip of filopodia in HeLa cells and that deletion of the kinase domain reduced cytoplasmic localization while enhancing localization at the tips of filopodia, suggesting that the kinase domain inhibits localization to filopodial tips (Erickson et al. 2003).

Physiological Function

Deletion of Drosophila ninaC causes abnormal phototransduction and light- and age-dependent retinal degeneration (Porter et al. 1992). Deletion of the motor domain leads to a change in the subcellular distribution of myosin III and a phenotype indistinguishable from that of a null mutant, while mutations in the kinase domain result in defects in normal phototransduction, but no retinal degeneration (Porter and Montell 1993). The study using temperature-sensitive mutants demonstrated that the motor domain is required for maintenance of the retinal structure, but not for normal phototransduction (Porter and Montell 1993).

Myosin III is a major calmodulin-binding protein and the distribution of calmodulin in the retina of Drosophila is dependent on myosin III expression (Porter et al. 1993). In the mutant lacking p174, which is specifically localized to rhabdomeres, microvilli-packed structures in photoreceptor cells, calmodulin does not concentrate in rhabdomeres, whereas deletion of p132, the cell body–specific isoform, results in a decrease in cytoplasmic calmodulin.

Recent studies suggested that myosin III is involved in the transport of signaling molecules that undergo light-dependent translocation between the rhabdomere and the cell body in Drosophila photoreceptors. Upon light illumination, visual arrestin Arr2 translocates into rhabdomeres from the cell body and inactivates rhodopsin. Arr2 binds to myosin III mediated by phosphoinositides and this translocation is hindered in myosin III null and p132-lacking mutants (Lee and Montell 2004). However, another group demonstrated that light-dependent translocation of Arr2 does not require myosin III (Satoh and Ready 2005).

Gqα, an activator of phospholipase Cβ, translocates from the rhabdomere to the cell body in response to light stimulation and returns to the rhabdomere in the dark. The rate of translocation from the rhabdomere to the cell body in myosin III null mutants is similar to that of wild type; however, upon return to the dark, transport to the rhabdomere is significantly slower in myosin III mutants (Cronin et al. 2004).

Localization of transient receptor potential-like (TRPL) channels in the rhabdomere of myosin III mutants raised in the dark resembles that of wild type, whereas in orange light translocation to the cell body is partially inhibited in myosin III null mutants (Meyer et al. 2006).

One of the binding partners of myosin III in Drosophila is INAD, a scaffolding protein for signalplex consisting of phospholipase C, protein kinase C, and calmodulin. Disruption of binding between myosin III and INAD leads to defects in the termination of the photoresponse (Wes et al. 1999).

Vertebrate myosin IIIA is expressed at high levels in the retina and at lower level in the brain and testis. It localizes to the calycal processes of rod and cone photoreceptors (Dose et al. 2003).

Transgene expression of GFP-tagged myosin IIIA in Xenopus rod photoreceptors produces abnormal calycal processes and causes subsequent rod degeneration (Lin-Jones et al. 2004). Transgene-induced long and thick calycal processes contain a larger number of actin bundles, suggesting that myosin III regulates the organization of the actin-cytoskeleton in the rod photoreceptors.

Mutations in myosin III found in an Israeli family cause nonsyndromic progressive hearing loss, but have no effect on vision, although myosin III is strongly expressed in retina (Walsh et al. 2002). Myosin IIIA localizes at the tip of stereocilia, which are actin protrusions found on the sensory hair cells in the inner ear, in the thimble-like pattern (Schneider et al. 2006). Overexpression of GFP-tagged full-length myosin IIIA localizes similar to endogenous myosin IIIA and does not change stereocilia structure; however a mutant lacking the kinase domain accumulates at a higher level at the tip and leads to elongation of the stereocilia and bulging of the tip (Schneider et al. 2006). Myosin IIIA colocalizes with espin 1, an actin-bundling protein, at stereocilia tips (Salles et al. 2009). Espin 1 interacts with 3THD1 of myosin IIIA and when coexpressed with myosin IIIA produces longer stereocilia suggesting that myosin IIIA regulates the length of stereocilia together with espin 1. Normal vision and late-onset deafness in patients with myosin IIIA mutations suggests that myosin IIIB, which has 3THDI but not 3THDII compensates for myosin IIIA in photoreceptors and partially in the inner ear.

Summary

Myosin III is a unique myosin in that it has a kinase domain at the N-terminus of its motor domain. Physiological functions of class III myosins have not been fully uncovered, especially in vertebrates; however, the production of model animals such as myosin III knockout or transgenic mice will provide key information regarding the roles of myosin III.

References

  1. Battelle BA, Andrews AW, Calman BG, Sellers JR, Greenberg RM, Smith WC. A myosin III from Limulus eyes is a clock-regulated phosphoprotein. J Neurosci. 1998;18(12):4548–59.PubMedGoogle Scholar
  2. Cronin MA, Diao F, Tsunoda S. Light-dependent subcellular translocation of Gqalpha in Drosophila photoreceptors is facilitated by the photoreceptor-specific myosin III NINAC. J Cell Sci. 2004;117(Pt 20):4797–806.PubMedCrossRefGoogle Scholar
  3. Dose AC, Burnside B. Cloning and chromosomal localization of a human class III myosin. Genomics. 2000;67(3):333–42.PubMedCrossRefGoogle Scholar
  4. Dose AC, Burnside B. A class III myosin expressed in the retina is a potential candidate for Bardet-Biedl syndrome. Genomics. 2002;79(5):621–4.PubMedCrossRefGoogle Scholar
  5. Dose AC, Hillman DW, Wong C, Sohlberg L, Lin-Jones J, Burnside B. Myo3A, one of two class III myosin genes expressed in vertebrate retina, is localized to the calycal processes of rod and cone photoreceptors and is expressed in the sacculus. Mol Biol Cell. 2003;14(3):1058–73.PubMedPubMedCentralCrossRefGoogle Scholar
  6. Dose AC, Ananthanarayanan S, Moore JE, Corsa AC, Burnside B, Yengo CM. The kinase domain alters the kinetic properties of the myosin IIIA motor. Biochemistry. 2008;47(8):2485–96.PubMedCrossRefGoogle Scholar
  7. Erickson FL, Corsa AC, Dose AC, Burnside B. Localization of a class III myosin to filopodia tips in transfected HeLa cells requires an actin-binding site in its tail domain. Mol Biol Cell. 2003;14(10):4173–80.PubMedPubMedCentralCrossRefGoogle Scholar
  8. Hicks JL, Liu X, Williams DS. Role of the ninaC proteins in photoreceptor cell structure: ultrastructure of ninaC deletion mutants and binding to actin filaments. Cell Motil Cytoskeleton. 1996;35(4):367–79.PubMedCrossRefGoogle Scholar
  9. Kambara T, Komaba S, Ikebe M. Human myosin III is a motor having an extremely high affinity for actin. J Biol Chem. 2006;281(49):37291–301.PubMedCrossRefGoogle Scholar
  10. Kempler K, Toth J, Yamashita R, Mapel G, Robinson K, Cardasis H, et al. Loop 2 of limulus myosin III is phosphorylated by protein kinase A and autophosphorylation. Biochemistry. 2007;46(14):4280–93.PubMedPubMedCentralCrossRefGoogle Scholar
  11. Komaba S, Inoue A, Maruta S, Hosoya H, Ikebe M. Determination of human myosin III as a motor protein having a protein kinase activity. J Biol Chem. 2003;278(24):21352–60.PubMedCrossRefGoogle Scholar
  12. Komaba S, Watanabe S, Umeki N, Sato O, Ikebe M. Effect of phosphorylation in the motor domain of human myosin IIIA on its ATP hydrolysis cycle. Biochemistry. 2009;49(17):3695–702.CrossRefGoogle Scholar
  13. Lee SJ, Montell C. Light-dependent translocation of visual arrestin regulated by the NINAC myosin III. Neuron. 2004;43(1):95–103.PubMedCrossRefGoogle Scholar
  14. Li HS, Porter JA, Montell C. Requirement for the NINAC kinase/myosin for stable termination of the visual cascade. J Neurosci. 1998;18(23):9601–6.PubMedGoogle Scholar
  15. Lin-Jones J, Parker E, Wu M, Dose A, Burnside B. Myosin 3A transgene expression produces abnormal actin filament bundles in transgenic Xenopus laevis rod photoreceptors. J Cell Sci. 2004;117(Pt 24):5825–34.PubMedCrossRefGoogle Scholar
  16. Meyer NE, Joel-Almagor T, Frechter S, Minke B, Huber A. Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade. J Cell Sci. 2006;119(Pt 12):2592–603.PubMedPubMedCentralCrossRefGoogle Scholar
  17. Montell C, Rubin GM. The Drosophila ninaC locus encodes two photoreceptor cell specific proteins with domains homologous to protein kinases and the myosin heavy chain head. Cell. 1988;52(5):757–72.PubMedCrossRefGoogle Scholar
  18. Ng KP, Kambara T, Matsuura M, Burke M, Ikebe M. Identification of myosin III as a protein kinase. Biochemistry. 1996;35(29):9392–9.PubMedCrossRefGoogle Scholar
  19. Porter JA, Montell C. Distinct roles of the Drosophila ninaC kinase and myosin domains revealed by systematic mutagenesis. J Cell Biol. 1993;122(3):601–12.PubMedCrossRefGoogle Scholar
  20. Porter JA, Hicks JL, Williams DS, Montell C. Differential localizations of and requirements for the two Drosophila ninaC kinase/myosins in photoreceptor cells. J Cell Biol. 1992;116(3):683–93.PubMedCrossRefGoogle Scholar
  21. Porter JA, Yu M, Doberstein SK, Pollard TD, Montell C. Dependence of calmodulin localization in the retina on the NINAC unconventional myosin. Science. 1993;262(5136):1038–42.PubMedCrossRefGoogle Scholar
  22. Salles FT, Merritt Jr RC, Manor U, Dougherty GW, Sousa AD, Moore JE, et al. Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments. Nat Cell Biol. 2009;11(4):443–50.PubMedPubMedCentralCrossRefGoogle Scholar
  23. Satoh AK, Ready DF. Arrestin1 mediates light-dependent rhodopsin endocytosis and cell survival. Curr Biol. 2005;15(19):1722–33.PubMedCrossRefGoogle Scholar
  24. Schneider ME, Dose AC, Salles FT, Chang W, Erickson FL, Burnside B, et al. A new compartment at stereocilia tips defined by spatial and temporal patterns of myosin IIIa expression. J Neurosci. 2006;26(40):10243–52.PubMedCrossRefGoogle Scholar
  25. Walsh T, Walsh V, Vreugde S, Hertzano R, Shahin H, Haika S, et al. From flies’ eyes to our ears: mutations in a human class III myosin cause progressive nonsyndromic hearing loss DFNB30. Proc Natl Acad Sci U S A. 2002;99(11):7518–23.PubMedPubMedCentralCrossRefGoogle Scholar
  26. Wes PD, XZ X, Li HS, Chien F, Doberstein SK, Montell C. Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD. Nat Neurosci. 1999;2(5):447–53.PubMedCrossRefGoogle Scholar

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© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Boston Biomedical Research InstituteWatertownUSA